-
Product Name
Anti-Actopaxin antibody
- Documents
-
Description
Rabbit polyclonal to Actopaxin
-
Tested applications
ELISA, WB, IP
-
Species reactivity
Human Actopaxin
-
Alternative names
2010012A22Rik antibody; 5430400F08Rik antibody; actopaxin antibody; actopaxin antibody; Actp antibody; AI225929 antibody; alpha-parvin antibody; alpha-parvin antibody; AU042898 antibody; calponin-like integrin-linked kinase-binding protein antibody; CH-ILKBP antibody; CH-ILKBP antibody; matrix-remodeling-associated protein 2 antibody; MNCb-0301 antibody; MXRA2 antibody; Parvin antibody; MXRA2 antibody; CH-ILKBP antibody; Actp antibody; Parvin antibody; AI225929 antibody; AU042898 antibody; CH-ILKBP antibody; 2010012A22Rik antibody; 5430400F08Rik antibody
-
Isotype
Rabbit IgG
-
Preparation
Produced in rabbits immunized with purified, recombinant Human Actopaxin (rh Actopaxin; Q9NVD7-1; Met1-Glu372). Actopaxin specific IgG was purified by Human Actopaxin affinity chromatography.
-
Clonality
Polyclonal
-
Formulation
0.2 μm filtered solution in PBS
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human Actopaxin. The detection limit for Human Actopaxin is < 0.039 ng/well.
IP: 1-4 μg/mg of lysate
-
Background
Actopaxin, also known as alpha-parvin, belongs to the parvin family. It is widely expressed, with highest levels in heart, skeletal muscle, kidney and liver. Actopaxin contains 2 CH (calponin-homology) domains and probably plays a role in the regulation of cell adhesion and cytoskeleton organization. It interacts with integrin-linked protein kinase and probably with actin and the LD1 and LD4 motifs of PXN. Actopaxin binds directly to both F-actin and paxillin LD1 and LD4 motifs. Actopaxin also exhibits robust focal adhesion localization in several cultured cell types but is not found along the length of the associated actin-rich stress fibers. It is absent from actin-rich cell-cell adherens junctions.
-
References
- Korenbaum E, et al. (2002) Genomic organization and expression profile of the parvin family of focal adhesion proteins in mice and humans. Gene. 279(1):69-79.
- Nikolopoulos SN, et al. (2002) Molecular dissection of actopaxin-integrin-linked kinase-Paxillin interactions and their role in subcellular localization. J Biol Chem. 277(2): 1568-75.
- Tu Y, et al. (2001) A new focal adhesion protein that interacts with integrin-linked kinase and regulates cell adhesion and spreading. J Cell Biol. 153(3): 585-98.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"