Human ACE2 / Angiotensin-Converting Enzyme 2 (Fc Tag) recombinant protein

Human ACE2 / Angiotensin-Converting Enzyme 2 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.

Angiotensin-converting enzyme 2

ACE2; ACEH; DKFZP434A014; RP23-330O24.1; 2010305L05RIK

Q9BYF1

ACE2; UNQ868/PRO1885

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Ser 740) of human ACE2 precursor (NP_068576.1) was expressed with the fused Fc region of human IgG1 at the C-terminus.

Human

The recombinant human ACE2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 961 amino acids and predicts a molecular mass of 110.3 kDa. As a result of glycosylation, the rhACE2/Fc monomer migrates as approximately 145-150 kDa band in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

1. Using the Octet RED System, the affinity constant (Kd) of human AEC2-Fc (Cat: 501568) bound to Spike (HCoV-EMC/2012) (Cat: 503810) was 6 nM.
2. Using the Octet RED System, the affinity constant (Kd) of human AEC2-Fc (Cat: 501568) bound to Spike (HCoV-EMC/2012) (Cat: 503542) was 30 nM.
3. Using the Octet RED System, the affinity constant (Kd) of human AEC2-Fc (Cat: 501568) bound to Spike (HCoV-EMC/2012) (ECD, aa 1-1297) (Cat: 503176) was 30 nM.
4. Using the Octet RED System, the affinity constant (Kd) of human AEC2-Fc (Cat: 501568) bound to Spike-His (aa 1-760) (Cat: 500181) was 10 nM.