Recombinant AMACR protein (His tag)

• DataSheet
• Material Safety Data Sheets (MSDS)

Recombinant Alpha-methylacyl-CoA racemase (EC 5.1.99.4), also known as 2-methylacyl-CoA racemase, is a high-purity protein fragment (residues 1-382) derived from human AMACR. This fragment is designed for structural and biochemical studies, offering a stable and soluble unit that retains functional relevance for investigating stereoisomer interconversion of alpha-methyl-branched-chain fatty acyl-CoA esters.

Alpha-methylacyl-CoA racemase, encoded by the AMACR gene in Homo sapiens, catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters. It acts exclusively on coenzyme A thioesters, not free fatty acids, and accepts a broad range of substrates including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (a bile acid synthesis intermediate), and arylpropionic acids like ibuprofen, while excluding 3-methyl-branched or linear-chain acyl-CoAs. This enzyme is localized to both peroxisomes and mitochondria, playing a critical role in lipid metabolism and bile acid biosynthesis, with implications in drug metabolism and inflammatory pathways.