alpha Tubulin antibody

Cat.#: 107873

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Product Information

  • Product Name
    alpha Tubulin antibody
  • Documents
  • Description
    alpha Tubulin Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human testis tissue, human brain tissue, human kidney tissue, human medulloblastoma tissue, human ovary tissue, human placenta tissue, human skin tissue. Positive WB detected in HeLa cells, mouse cerebellum tissue. Observed molecular weight by Western-blot: 50-55 kDa
  • Tested applications
    ELISA, IHC, IF, WB
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    alpha tubulin antibody; TUBA1A antibody; TUBA3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of alpha Tubulin recombinant protein (Accession Number: NM_006009). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:117300(tubulin-Alpha antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:117300(tubulin-Alpha antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human testis using Catalog No:117300(tubulin-Alpha antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:117300(tubulin-Alpha antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:117300(tubulin-Alpha antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:117300(tubulin-Alpha antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells using Catalog No:117300(tubulin-Alpha Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

    Immunofluorescent analysis of HepG2 cells using Catalog No:117300(tubulin-Alpha Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

  • Background
    TUBA1A represents tubulin, alpha 1a
  • References
    • Shen E, Lei Y, Liu Q. Identification and characterization of INMAP, a novel interphase nucleus and mitotic apparatus protein that is involved in spindle formation and cell cycle progression. Experimental cell research. 315(7):1100-16. 2009.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"