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Product Name
Alpha-1-Antitrypsin antibody
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Description
Alpha-1-Antitrypsin Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue. Positive FC detected in HepG2 cells. Positive WB detected in mouse kidney tissue, human kidney tissue, mouse liver tissue, mouse lung tissue, mouse skin tissue. Positive IP detected in mouse kidney tissue. Observed molecular weight by Western-blot: 40 kDa,45-53 kDa
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Tested applications
ELISA, IHC, WB, IP, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
A1A antibody; A1AT antibody; AAT antibody; Alpha 1 antiproteinase antibody; Alpha 1 antitrypsin antibody; Alpha 1 protease inhibitor antibody; PI1 antibody; PRO2209 antibody; PRO2275 antibody; Serpin A1 antibody; SERPINA1 antibody; SPAAT antibody; α1-antitrypsin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Alpha-1-Antitrypsin recombinant protein (Accession Number: NM_001127700). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:107875(Alpha-1-Antitrypsin antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:107875(Alpha-1-Antitrypsin antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:107875(Alpha-1-Antitrypsin antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-Alpha-1-Antitrypsin (IP:Catalog No:107875, 4ug; Detection:Catalog No:107875 1:1000) with mouse kidney tissue lysate 7200ug.
1X10^6 HepG2 cells were stained with 0.2ug Alpha-1-Antitrypsin antibody (Catalog No:107875, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.
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References
- Qin X, Chen Q, Sun C. High-throughput screening of tumor metastatic-related differential glycoprotein in hepatocellular carcinoma by iTRAQ combines lectin-related techniques. Medical oncology (Northwood, London, England). 30(1):420. 2013.
- He T, Hu JY, Han J. Identification of differentially expressed serum proteins in infectious purpura fulminans. Disease markers. 2014:698383. 2014.
- Ludwig MR, Kojima K, Bowersock GJ. Surveying the serologic proteome in a tissue-specific kras(G12D) knockin mouse model of pancreatic cancer. Proteomics. 16(3):516-31. 2016.
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