Recombinant AKTS1 protein (His tag)

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Recombinant Human Proline-rich AKT1 substrate 1 (AKT1S1) fragment (residues 1-82) is a high-purity protein designed for structural and biochemical studies. This 82-amino acid fragment represents a stable, soluble unit of the full-length protein, which serves as a key negative regulator of mTORC1 signaling—a central pathway controlling cellular growth and metabolism.

Proline-rich AKT1 substrate 1 (AKT1S1) is a human protein that functions as a critical negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved nutrient sensor that promotes anabolic reactions and macromolecule biosynthesis to drive cellular growth. In the absence of insulin and nutrients, AKT1S1 associates with mTORC1 and directly inhibits its activity by blocking the substrate-recruitment site. Upon insulin or nutrient stimulation, AKT1S1 dissociates from mTORC1, with this regulatory mechanism being dependent on its phosphorylation state and interaction with 14-3-3 proteins. AKT1S1 is primarily localized in the cytoplasm and cytosol, and it may also contribute to nerve growth factor-mediated neuroprotection. The provided fragment (residues 1-82) was selected computationally to ensure stability and solubility, enabling detailed investigation of AKT1S1's functional properties.