AGFG1 Polyclonal Antibody

Cat.#: 161656

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Product Information

  • Product Name
    AGFG1 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to AGFG1
  • Tested applications
    WB, IF
  • Species reactivity
    Human, Mouse
  • Alternative names
    AGFG1 antibody; HRB antibody; RAB antibody; RIP antibody; ArfGAP with FG repeats 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human AGFG1 (NP_004495.2).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IF 1:50 - 1:200
  • Validations

    Western blot - AGFG1 Polyclonal Antibody

    Western blot - AGFG1 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using AGFG1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 30s.

    Immunofluorescence - AGFG1 Polyclonal Antibody

    Immunofluorescence - AGFG1 Polyclonal Antibody

    Immunofluorescence analysis of HeLa cells using AGFG1 antibody . Blue: DAPI for nuclear staining.

  • Background
    Required for vesicle docking or fusion during acrosome biogenesis (By similarity). May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element-containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"