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Product Name
ACO1 Polyclonal Antibody
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Description
Polyclonal antibody to ACO1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
ACO1 antibody; ACONS antibody; HEL60 antibody; IREB1 antibody; IREBP antibody; IREBP1 antibody; IRP1 antibody; aconitase 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human ACO1 (NP_002188.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - ACO1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ACO1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunohistochemistry - ACO1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human prostate using ACO1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ACO1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using ACO1 antibody at dilution of 1:100 (40x lens).
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Background
Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.; Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"