Recombinant ACADM protein (His tag)

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Recombinant human Medium-chain specific acyl-CoA dehydrogenase, mitochondrial (MCAD) fragment (residues 35-421) is a high-purity, stable protein designed for structural and functional studies. This 387-amino acid fragment retains key biochemical properties, making it an ideal tool for investigating fatty acid beta-oxidation mechanisms and enzyme kinetics in metabolic research.

Medium-chain specific acyl-CoA dehydrogenase (MCAD), encoded by the ACADM gene, catalyzes the initial step in mitochondrial fatty acid beta-oxidation—a critical energy-producing pathway that breaks down fats into acetyl-CoA. Specifically, MCAD removes hydrogen atoms from C-2 and C-3 of saturated medium-chain acyl-CoAs (6–12 carbons), generating trans-2-enoyl-CoA while transferring electrons to the respiratory chain via electron transfer flavoprotein (ETF). Located in the mitochondrial matrix, MCAD enables aerobic energy derivation from lipids, and its dysfunction is linked to metabolic disorders. The recombinant fragment (35-421) provides a soluble, stable unit for elucidating MCAD's role in cellular metabolism.