3 x DYKDDDDK peptide

3 x DYKDDDDK peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-DYKDDDDKDYKDDDDKDYKDDDDK-OH

H-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH

24

95.15%

C₁₂₃H₁₇₆N₃₀O₅₈

3002.86

Synthetic

Powder

The DYKDDDDK peptide (peptide sequence: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, including an enterokinase interface) has been specially developed for immunoaffinity chromatography. The peptide allows the competitive elution of proteins under mild, non-denaturating conditions. Various antibodies to the peptide have been developed. An antibody called M1 binds the peptide in the presence of bivalent metal ions, preferably Ca2+. Elution occurs by complexing agent Ca2+ or with an excess of free DYKDDDDK peptide.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Koskela MM, Skotnicová P, Kiss É, Sobotka R. Purification of Protein-complexes from the Cyanobacterium Synechocystis sp. PCC 6803 Using FLAG-affinity Chromatography. Bio Protoc. 2020 May 20;10(10):e3616. doi: 10.21769/BioProtoc.3616. PMID: 33659289; PMCID: PMC7842675.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.