3 x DYKDDDDK peptide

3 x DYKDDDDK peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-DYKDDDDKDYKDDDDKDYKDDDDK-OH

H-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH

24

95.15%

C₁₂₃H₁₇₆N₃₀O₅₈

3002.86

Synthetic

Powder

The DYKDDDDK peptide (peptide sequence: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, including an enterokinase interface) has been specially developed for immunoaffinity chromatography. The peptide allows the competitive elution of proteins under mild, non-denaturating conditions. Various antibodies to the peptide have been developed. An antibody called M1 binds the peptide in the presence of bivalent metal ions, preferably Ca2+. Elution occurs by complexing agent Ca2+ or with an excess of free DYKDDDDK peptide.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Koskela MM, Skotnicová P, Kiss É, Sobotka R. Purification of Protein-complexes from the Cyanobacterium Synechocystis sp. PCC 6803 Using FLAG-affinity Chromatography. Bio Protoc. 2020 May 20;10(10):e3616. doi: 10.21769/BioProtoc.3616. PMID: 33659289; PMCID: PMC7842675.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.