[Lys5,MeLeu9,Nle10]Neurokinin A(4-10) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

The peptide [Lys5,MeLeu9,Nle10]Neurokinin A(4-10) (LMN-NKA) is a selective agonist of the neurokinin NK2 receptor, exhibiting high specificity in binding and functional assays. It demonstrates a 674-fold selectivity for NK2 over NK1 receptors in radioligand binding assays and ≥74-fold selectivity in functional in vitro assays. LMN-NKA induces contractile activity in visceral smooth muscle, particularly in the colon and urinary bladder, across various species including rats, dogs, pigs, and humans.

In experimental models, LMN-NKA rapidly increases bladder and colorectal pressures upon administration, with effects mediated through NK2 receptor activation. The peptide's pharmacological profile includes dose-dependent increases in peak bladder and colorectal pressures, with rapid onset and sustained duration of action. These effects are inhibited by pretreatment with the NK2 receptor antagonist GR 159897, confirming receptor specificity. The peptide's activity is conserved across species, making it a valuable tool for studying NK2 receptor-mediated physiological responses.

• Rupniak NMJ, Katofiasc MA, Marson L, Ricca DJ, Thor KB, Burgard EC. Prokinetic effects of the neurokinin NK2 receptor agonist [Lys5,MeLeu9,Nle10]-NKA(4-10) on bladder and colorectal activity in minipigs. Neuropeptides. 2019 Oct;77:101956. doi: 10.1016/j.npep.2019.101956. Epub 2019 Jul 11. PMID: 31324387; PMCID: PMC6739136.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.