(klaklak)2 peptide

(klaklak)2 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-KLAKLAKKLAKLAK-OH (All D-type amino acid)

H-Lys-Leu-Ala-Lys-Leu-Ala-Lys-Lys-Leu-Ala-Lys-Leu-Ala-Lys-OH (All D-type amino acid)

14

98.45%

C₇₂H₁₃₈N₂₀O₁₅

1523.99

Synthetic

Powder

(KLAKLAK)2 (referred to as KLAK from here) is an antimicrobial peptide known to induce apoptosis via mitochondrial membrane disruption. KLAK exerts low cytotoxicity to mammalian cells owing to its low internalization capacity and thus requires an internalizing peptide for effective anticancer activity. KLAK has been conjugated to several peptides such as RGD-4C and NGR, NRP-1 binding peptide (CGFYWLRSC), and bladder tumor homing nonapeptide (Bld-1; CSNRDARRC). Conjugation strategy that utilizes the coupling of pro-apoptotic KLAK peptide either to the C- or the N-terminus of the targeting peptide have been used. Most of the peptides identified using the T7 phage display system use the N-terminus for conjugation. However, it is important not to interfere with the binding site of the targeting peptide while considering the conjugation strategy. The iron oxide nanoworms were functionalized by conjugation of the D(KLAKLAK)2 to the N-terminus of the p32 targeting tumor penetrating peptide (LinTT1; AKRGARSTA through a polyethylene glycol (PEG) linker (NW-PEG-Cys-D(KLAKLAK)2-X-LinTT1). The conjugate showed significantly improved anticancer activity in several p32-expressing peritoneal tumor models. Modification of the N-terminus of glioma homing peptide (CooP; CGLSGLGVA) by introduction of alanine and TAMRA fluorophore (TAMRA-ACooP) did not affect CooP homing in vivo using glioblastoma model.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Kim HY, Kim S, Youn H, Chung JK, Shin DH, Lee K. The cell penetrating ability of the proapoptotic peptide, KLAKLAKKLAKLAK fused to the N-terminal protein transduction domain of translationally controlled tumor protein, MIIYRDLISH. Biomaterials. 2011;32(22):5262-8

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.