[Glu2]TRH peptide

[Glu2]TRH peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Pyr-EP-NH2

Pyr-Glu-Pro-NH2

2

95.14%

C₁₅H₂₂N₄O₆

354.36

85541-78-2

122148

Synthetic

Powder

[Glu2]TRH is an endogenous peptide with the amino acid sequence pGlu-Glu-Pro-NH2. It is structurally related to thyrotropin-releasing hormone (TRH, pGlu-His-Pro-NH2). [Glu2]TRH is not metabolized by thyroliberinase, an enzyme that rapidly inactivates TRH, and is stable in rat serum for at least 4 hours, being cleared only by the kidneys. [Glu2]TRH does not bind with an appreciable affinity to the two known TRH-receptor isoforms (TRHR1 and TRHR2). [Glu2]TRH possesses intrinsic analeptic activity, but its analeptic action is less efficacious than that of TRH. In mice, it dose-dependently attenuates the TRH-evoked reversal of barbiturate-induced sleeping time. [Glu2]TRH does not possess an intrinsic ACh-stimulatory effect and opposes the cholinergic effect of TRH in the rat CNS. [Glu2]TRH has been reported to have neuroprotective, antidepressant, anticonvulsant, and other activities in the CNS. Many centrally-mediated effects of TRH are shared with [Glu2]TRH, but they may involve different receptors or mechanisms. For example, [Glu2]TRH does not have TSH-releasing activity and does not elevate thyroid-hormone levels. The diastereomer [D-Glu2]TRH is neither an analeptic nor an inhibitor/negative modulator in the context of the analeptic effect and ACh release. [Glu2]TRH may be considered as a negative modulator for the cholinergic effect of TRH in the mouse brain and has the potential to be used as a lead for the development of pharmacological agents to understand the cholinergic mechanisms of TRH's CNS effects.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Nguyen V, Zharikova AD, Prokai-Tatrai K, Prokai L. [Glu2]TRH dose-dependently attenuates TRH-evoked analeptic effect in mice. Brain Res Bull. 2010 Apr 29;82(1-2):83-6. doi: 10.1016/j.brainresbull.2010.02.007. Epub 2010 Feb 24. PMID: 20188155; PMCID: PMC2867048.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.