pSiM24 vector (V003182)

LOCUS       40924_40417        7081 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pSiM24, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Sahoo DK, Dey N, Maiti IB.
  TITLE     pSiM24 Is a Novel Versatile Gene Expression Vector for Transient 
            Assays As Well As Stable Expression of Foreign Genes in Plants
  JOURNAL   PLoS ONE 9 (6), E98988 (2014)
  PUBMED    24897541
REFERENCE   2  (bases 1 to 7081)
  AUTHORS   Maiti IB, Sahoo DK.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2013) KTRDC, Plant Genetic Engineering Research 
            and Services, College of Agriculture, University of Kentucky, 1401 
            University Drive, Lexington, KY 40546, USA
REFERENCE   3  (bases 1 to 7081)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7081)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2014"; volume: "9"; issue: "6"; pages: "E98988"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-MAY-2013) KTRDC, Plant Genetic Engineering Research and 
            Services, College of Agriculture, University of Kentucky, 1401 
            University Drive, Lexington, KY 40546, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(66..90)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    170..234
                     /label=1st polylinker/multiple cloning site
                     /note="1st polylinker/multiple cloning site"
     regulatory      235..860
                     /note="M24 promoter; derived from Mirabilis Mosaic Virus
                     (MMV)"
                     /regulatory_class="promoter"
     misc_feature    861..919
                     /label=2nd polylinker/multiple cloning site
                     /note="2nd polylinker/multiple cloning site"
     terminator      919..1560
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     terminator      complement(1571..1821)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1824..2612)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(2640..2819)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    2903..2956
                     /label=3rd linker/multiple cloning site
                     /note="3rd linker/multiple cloning site"
     misc_feature    complement(3054..3078)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(3193..3781)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     3'UTR           complement(3805..3951)
                     /gene="bla"
                     /label=similar to INSD accession XM2327
                     /note="similar to INSD accession XM2327"
     CDS             complement(3955..4812)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4813..4917)
                     /label=AmpR promoter
     rep_origin      4946..5576
                     /label=oriV
                     /note="incP origin of replication"
     regulatory      5577..5880
                     /gene="pRK2-trfA"
                     /regulatory_class="promoter"
     CDS             5881..7026
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     3'UTR           7030..7081
                     /gene="pRK2-trfA"
                     /label=trfA-terminator
                     /note="trfA-terminator"