LOCUS       40924_40417        7081 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pSiM24, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Sahoo DK, Dey N, Maiti IB.
  TITLE     pSiM24 Is a Novel Versatile Gene Expression Vector for Transient 
            Assays As Well As Stable Expression of Foreign Genes in Plants
  JOURNAL   PLoS ONE 9 (6), E98988 (2014)
  PUBMED    24897541
REFERENCE   2  (bases 1 to 7081)
  AUTHORS   Maiti IB, Sahoo DK.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2013) KTRDC, Plant Genetic Engineering Research 
            and Services, College of Agriculture, University of Kentucky, 1401 
            University Drive, Lexington, KY 40546, USA
REFERENCE   3  (bases 1 to 7081)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7081)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2014"; volume: "9"; issue: "6"; pages: "E98988"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-MAY-2013) KTRDC, Plant Genetic Engineering Research and 
            Services, College of Agriculture, University of Kentucky, 1401 
            University Drive, Lexington, KY 40546, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(66..90)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    170..234
                     /label=1st polylinker/multiple cloning site
                     /note="1st polylinker/multiple cloning site"
     regulatory      235..860
                     /note="M24 promoter; derived from Mirabilis Mosaic Virus
                     (MMV)"
                     /regulatory_class="promoter"
     misc_feature    861..919
                     /label=2nd polylinker/multiple cloning site
                     /note="2nd polylinker/multiple cloning site"
     terminator      919..1560
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     terminator      complement(1571..1821)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1824..2612)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(2640..2819)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    2903..2956
                     /label=3rd linker/multiple cloning site
                     /note="3rd linker/multiple cloning site"
     misc_feature    complement(3054..3078)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(3193..3781)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     3'UTR           complement(3805..3951)
                     /gene="bla"
                     /label=similar to INSD accession XM2327
                     /note="similar to INSD accession XM2327"
     CDS             complement(3955..4812)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4813..4917)
                     /label=AmpR promoter
     rep_origin      4946..5576
                     /label=oriV
                     /note="incP origin of replication"
     regulatory      5577..5880
                     /gene="pRK2-trfA"
                     /regulatory_class="promoter"
     CDS             5881..7026
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     3'UTR           7030..7081
                     /gene="pRK2-trfA"
                     /label=trfA-terminator
                     /note="trfA-terminator"
ORIGIN
        1 gcgcgcggta cccagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc
       61 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag
      121 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccactagac cagtaccttg
      181 gaggcctgtg cgcatcccag ggtgggtccc gtataccccg gggaattcgt caacttcgtc
      241 cacagacatc aacatcttat cgtcctttga agataagata ataatgttga agataagagt
      301 gggagccacc actaaaacat tgctttgtca aaagctaaaa aagatgatgc ccgacagcca
      361 cttgtgtgaa gcatgtgaag ccggtccctc cactaagaaa attagtgaag catcttccag
      421 tggtccctcc actcacagct caatcagtga gcaacaggac gaaggaaatg acgtaagcca
      481 tgacgtctaa tccccccaac ttcgtccaca gacatcaaca tcttatcgtc ctttgaagat
      541 aagataataa tgttgaagat aagagtggga gccaccacta aaacattgct ttgtcaaaag
      601 ctaaaaaaga tgatgcccga cagccacttg tgtgaagcat gtgaagccgg tccctccact
      661 aagaaaatta gtgaagcatc ttccagtggt ccctccactc acagctcaat cagtgagcaa
      721 caggacgaag gaaatgacgt aagccatgac gtctaatccc acaagaattt ccttatataa
      781 ggaacacaaa tcagaaggaa gagatcaatc gaaatcaaaa tcggaatcga aatcaaaatc
      841 ggaatcgaaa tctctcatct aagcttggat ccctcgaggt taacacgcgt gtcgacgagc
      901 tcctgcagga tcctctagag ctttcgttcg tatcatcggt ttcgacaacg ttcgtcaagt
      961 tcaatgcatc agtttcattg cgcacacacc agaatcctac tgagttcgag tattatggca
     1021 ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt aatttactgt gttttttatt
     1081 cggttttcgc tatcgaactg tgaaatggaa atggatggag aagagttaat gaatgatatg
     1141 gtccttttgt tcattctcaa attaatatta tttgtttttt ctcttatttg ttgtgtgttg
     1201 aatttgaaat tataagagat atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt
     1261 ggcctctaat gaccgaagtt aatatgagga gtaaaacact tgtagttgta ccattatgct
     1321 tattcactag gcaacaaata tattttcaga cctagaaaag ctgcaaatgt tactgaatac
     1381 aagtatgtcc tcttgtgttt tagacattta tgaactttcc tttatgtaat tttccagaat
     1441 ccttgtcaga ttctaatcat tgctttataa ttatagttat actcatggat ttgtagttga
     1501 gtatgaaaat attttttaat gcattttatg acttgccaat tgattgacaa catgcatcaa
     1561 tcgatactag tctagtaaca tagatgacac cgcgagcgat aatttatcct agtttgcgag
     1621 ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac
     1681 ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa
     1741 cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta
     1801 ttgccaaatg tttgaacgat tcagaagaac tcatcgagga gcctgtagaa agcaatcctt
     1861 tgggaatctg gagcagcaat tccgtaaagc acaaggaatc tatcagccca ctctccacca
     1921 agttcttcag caatatccct agtagcgaga gcaatatcct gatacctatc agcaactcca
     1981 agccttccgc aatcaatgaa tccagaaaac cttccgttct ccaccataat gtttggaagg
     2041 caagcatcac catgagtaac aacaagatcc tcaccatctg gcattctagc cttgagccta
     2101 gcaaaaagtt cagctggagc caatccttga tgttcctcat caagatcatc ctgatccaca
     2161 agtccagctt ccatcctagt tctagccctt tcaattctgt gcttagcttg gtgatcaaat
     2221 gggcaagtag caggatcaag agtgtgaagc cttctcatag catcagccat aatggaaacc
     2281 ttctcagctg gagcaaggtg tgaagaaagc aaatcttgtc ctggaacctc tcccaaaaga
     2341 agccaatctc ttccagcctc agtaacaaca tcaagaacag cagcgcatgg aacaccagta
     2401 gtagcaagcc aagaaagtct agcagcctca tcttgaagct cattaagagc accagaaaga
     2461 tcagtcttaa cgaaaagaac tggccttccc tgagcagaaa gcctaaaaac agcagcatca
     2521 gagcatccaa tagtctgttg agcccaatcg tatccaaaaa gcctctcaac ccaagcagca
     2581 ggacttccag catgaagtcc atcttgctca attcccattt gagagtgaat atgagactct
     2641 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     2701 gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     2761 gcttagctca ttaaactcca gaaacccgcg gctcagtggc tccttcaacg ttgcggttct
     2821 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     2881 ccttaattct ccgctcatga tcagatctgg tgacccctat gtaaggggcc ggccatttaa
     2941 attcgcgatt aattaacctg tggttggcat gcacatacaa atggacgaac ggataaacct
     3001 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     3061 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     3121 tatcgcgcgc ggtacctgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     3181 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3241 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3301 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3361 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3421 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3481 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3541 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3601 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3661 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3721 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3781 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3841 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3901 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3961 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4021 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     4081 aatgataccg cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc
     4141 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     4201 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     4261 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     4321 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     4381 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4441 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4501 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4561 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4621 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4681 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4741 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4801 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4861 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4921 atttccccga aaagtgccac gggccccgct caccgggctg gttgccctcg ccgctgggct
     4981 ggcggccgtc tatggccctg caaacgcgcc agaaacgccg tcgaagccgt gtgcgagaca
     5041 ccgcggccgc cggcgttgtg gatacctcgc ggaaaacttg gccctcactg acagatgagg
     5101 ggcggacgtt gacacttgag gggccgactc acccggcgcg gcgttgacag atgaggggca
     5161 ggctcgattt cggccggcga cgtggagctg gccagcctcg caaatcggcg aaaacgcctg
     5221 attttacgcg agtttcccac agatgatgtg gacaagcctg gggataagtg ccctgcggta
     5281 ttgacacttg aggggcgcga ctactgacag atgaggggcg cgatccttga cacttgaggg
     5341 gcagagtgct gacagatgag gggcgcacct attgacattt gaggggctgt ccacaggcag
     5401 aaaatccagc atttgcaagg gtttccgccc gtttttcggc caccgctaac ctgtctttta
     5461 acctgctttt aaaccaatat ttataaacct tgtttttaac cagggctgcg ccctgtgcgc
     5521 gtgaccgcgc acgccgaagg ggggtgcccc cccttctcga accctcccgg cccgctgtcg
     5581 actacctaga tgtggcgcaa cgatgccggc gacaagcagg agcgcaccga cttcttccgc
     5641 atcaagtgtt ttggctctca ggccgaggcc cacggcaagt atttgggcaa ggggtcgctg
     5701 gtattcgtgc agggcaagat tcggaatacc aagtacgaga aggacggcca gacggtctac
     5761 gggaccgact tcattgccga taaggtggat tatctggaca ccaaggcacc aggcgggtca
     5821 aatcaggaat aagggcacat tgccccggcg tgagtcgggg caatcccgca aggagggtga
     5881 atgaatcgca ccttcgatcg caaggcgtat cgccaggaac tgatcgacgc cggcttctcg
     5941 gccgaagatg ccgaaacgat cgcctcgcgc accgtcatgc gtgccccgcg cgaaaccttc
     6001 cagtcggtcg gctcgatggt ccagcaggcc accgccaaga tcgaacgcga ttcggtccag
     6061 ctggccccgc cggcccttcc ggccccgtcg gccgccgtcg aacgctcgcg ccggctggaa
     6121 caggaagccg ccggcctggc caagtcgatg accatcgaca cccgcggcac catgaccacc
     6181 aagaagcgca agaccgccgg cgaggatctg gccaagcagg tcagcgaagc caagcaggcc
     6241 gccctgctga agcataccaa gcagcagatc aaggaaatgc agctgtcgct gttcgacatc
     6301 gccccgtggc cggataccat gcgggccatg ccgaatgata ccgcccgctc ggccctgttc
     6361 accacccgca ataagaagat cccgcgcgaa gccctccaga ataaggtcat cttccacgtc
     6421 aacaaggatg tcaagatcac ctataccggc gtcgaactgc gggccgatga tgatgaactg
     6481 gtctggcagc aggtcctgga atatgccaag cgcaccccga tcggcgaacc gatcaccttc
     6541 accttctatg aactgtgcca ggatctgggc tggtcgatca atggccgcta ttataccaag
     6601 gccgaagaat gcctgtcgcg cctccaggcg accgcgatgg gcttcacctc ggatcgcgtc
     6661 ggccatctgg aatcggtcag cctgctgcac cgcttccgcg tcctggatcg cggcaagaag
     6721 acctcgcgct gccaggtcct gatcgacgaa gaaatcgtcg tcctgttcgc cggcgatcat
     6781 tataccaagt tcatctggga gaagtatcgc aagctgtcgc cgaccgcccg ccgcatgttc
     6841 gattatttct cgtcgcaccg cgaaccgtat ccgctgaagc tggaaacctt ccgcctgatg
     6901 tgcggctcgg attcgacccg cgtcaagaag tggcgcgaac aggtcggcga agcctgcgaa
     6961 gaactgcgcg gctcgggcct ggtcgaacat gcctgggtca atgatgatct ggtccattgc
     7021 aagcgctagg gccttgtggg gtcagttccg gctgggggtt cagccagcgc tttacgatat
     7081 c
//
