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psiCHECK(TM)-2 vector (V003190#)

Price Information

Cat No. Plasmid Name Availability Add to cart
V003190 psiCHECK(TM)-2 In stock (lyophilized plasmid)

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Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

Basic Vector Information
Vector Name psiCHECK(TM)-2 Antibiotic Resistance Ampicillin
Length 6273 bp Type RNA interference vector
Source Almond BD, Vidugiriene J, Kobs G.

psiCHECK(TM)-2 vector Vector Map

SV40 early enhancer/promoter chimeric T7 RNA polymerase promoter vertebrate consensus sequence for strong initiation of translation (Kozak, 1987) multiple cloning site polyA HSV-TK promoter vertebrate consensus sequence for strong initiation of translation (Kozak, 1987) SV40 late polyA AmpR promoter beta-lactamase ori

psiCHECK(TM)-2 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                6273 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  RNA interference vector psiCHECK(TM)-2, complete sequence.
ACCESSION   AY535007
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Almond BD, Vidugiriene J, Kobs G.
  TITLE     psiCHECK(TM) Vectors Technical Bulletin, TB329
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6273)
  AUTHORS   Almond BD, Vidugiriene J, Kobs G.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2004) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711-5399, USA
REFERENCE   3  (bases 1 to 6273)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="RNA interference vector psiCHECK(TM)-2"
                     /mol_type="other DNA"
                     /db_xref="taxon:263992"
     regulatory      7..425
                     /regulatory_class="promoter"
                     /note="SV40 early enhancer/promoter"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          489..621
                     /note="chimeric"
     regulatory      666..684
                     /regulatory_class="promoter"
                     /note="T7 RNA polymerase promoter"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      688..697
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             694..1629
                     /codon_start=1
                     /product="luciferase"
                     /label=luciferase
                     /note="derived from Renilla"
                     /protein_id="AAS38484.1"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    1636..1680
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    1688..1736
                     /label=polyA
                     /note="polyA"
     polyA_signal    1688..1736
                     /note="synthetic polyadenylation signal"
     regulatory      1744..2496
                     /regulatory_class="promoter"
                     /note="HSV-TK promoter"
     promoter        1745..2496
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      2526..2535
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2532..4184
                     /codon_start=1
                     /product="luciferase"
                     /label=luciferase
                     /note="derived from firefly; hluc+"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             3491..3499
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    4219..4440
                     /label=SV40 late polyA
                     /note="SV40 late polyA"
     polyA_signal    4228..4349
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4482..4586
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4587..5447
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="Ampr"
                     /protein_id="AAS38486.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5618..6206
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

Plasmid Resuspension protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.