LOCUS 40924_40312 6273 bp DNA circular SYN 18-DEC-2018 DEFINITION RNA interference vector psiCHECK(TM)-2, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6273) AUTHORS Almond BD, Vidugiriene J, Kobs G. TITLE psiCHECK(TM) Vectors Technical Bulletin, TB329 JOURNAL Unpublished REFERENCE 2 (bases 1 to 6273) AUTHORS Almond BD, Vidugiriene J, Kobs G. TITLE Direct Submission JOURNAL Submitted (29-JAN-2004) Scientific Communications, Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711-5399, USA REFERENCE 3 (bases 1 to 6273) TITLE Direct Submission REFERENCE 4 (bases 1 to 6273) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (29-JAN-2004) Scientific Communications, Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711-5399, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..6273 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 62..419 /label=SV40 promoter /note="SV40 enhancer and early promoter" intron 489..621 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" promoter 666..684 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 694..1626 /codon_start=1 /label=hRluc /note="Renilla luciferase" /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" misc_feature 1636..1680 /label=multiple cloning site /note="multiple cloning site" polyA_signal 1688..1736 /label=poly(A) signal /note="synthetic polyadenylation signal" promoter 1745..2496 /label=HSV TK promoter /note="herpes simplex virus thymidine kinase promoter" CDS 2532..4181 /codon_start=1 /label=luciferase /note="firefly luciferase" /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" polyA_signal complement(4228..4349) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter 4482..4586 /label=AmpR promoter CDS 4587..5444 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 5618..6206 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct 61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca 481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct 541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc 601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga 661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag 721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg 781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt 841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc 901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat 961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac 1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac 1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg 1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa 1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag 1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag 1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc 1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat 1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga 1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag 1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac 1621 gagcagtaat tctaggcgat cgctcgagcc cgggaattcg tttaaaccta gagcggccgc 1681 tggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgagga 1741 tctaaatgag tcttcggacc tcgcgggggc cgcttaagcg gtggttaggg tttgtctgac 1801 gcggggggag ggggaaggaa cgaaacactc tcattcggag gcggctcggg gtttggtctt 1861 ggtggccacg ggcacgcaga agagcgccgc gatcctctta agcacccccc cgccctccgt 1921 ggaggcgggg gtttggtcgg cgggtggtaa ctggcgggcc gctgactcgg gcgggtcgcg 1981 cgccccagag tgtgaccttt tcggtctgct cgcagacccc cgggcggcgc cgccgcggcg 2041 gcgacgggct cgctgggtcc taggctccat ggggaccgta tacgtggaca ggctctggag 2101 catccgcacg actgcggtga tattaccgga gaccttctgc gggacgagcc gggtcacgcg 2161 gctgacgcgg agcgtccgtt gggcgacaaa caccaggacg gggcacaggt acactatctt 2221 gtcacccgga ggcgcgaggg actgcaggag cttcagggag tggcgcagct gcttcatccc 2281 cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt tgcatgtctt 2341 tagttctatg atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca 2401 gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga cgcgtgtggc 2461 ctcgaacacc gagcgaccct gcagcgaccc gcttaaaagc ttggcattcc ggtactgttg 2521 gtaaagccac catggccgat gctaagaaca ttaagaaggg ccctgctccc ttctaccctc 2581 tggaggatgg caccgctggc gagcagctgc acaaggccat gaagaggtat gccctggtgc 2641 ctggcaccat tgccttcacc gatgcccaca ttgaggtgga catcacctat gccgagtact 2701 tcgagatgtc tgtgcgcctg gccgaggcca tgaagaggta cggcctgaac accaaccacc 2761 gcatcgtggt gtgctctgag aactctctgc agttcttcat gccagtgctg ggcgccctgt 2821 tcatcggagt ggccgtggcc cctgctaacg acatttacaa cgagcgcgag ctgctgaaca 2881 gcatgggcat ttctcagcct accgtggtgt tcgtgtctaa gaagggcctg cagaagatcc 2941 tgaacgtgca gaagaagctg cctatcatcc agaagatcat catcatggac tctaagaccg 3001 actaccaggg cttccagagc atgtacacat tcgtgacatc tcatctgcct cctggcttca 3061 acgagtacga cttcgtgcca gagtctttcg acagggacaa aaccattgcc ctgatcatga 3121 acagctctgg gtctaccggc ctgcctaagg gcgtggccct gcctcatcgc accgcctgtg 3181 tgcgcttctc tcacgcccgc gaccctattt tcggcaacca gatcatcccc gacaccgcta 3241 ttctgagcgt ggtgccattc caccacggct tcggcatgtt caccaccctg ggctacctga 3301 tttgcggctt tcgggtggtg ctgatgtacc gcttcgagga ggagctgttc ctgcgcagcc 3361 tgcaagacta caaaattcag tctgccctgc tggtgccaac cctgttcagc ttcttcgcta 3421 agagcaccct gatcgacaag tacgacctgt ctaacctgca cgagattgcc tctggcggcg 3481 ccccactgtc taaggaggtg ggcgaagccg tggccaagcg ctttcatctg ccaggcatcc 3541 gccagggcta cggcctgacc gagacaacca gcgccattct gattacccca gagggcgacg 3601 acaagcctgg cgccgtgggc aaggtggtgc cattcttcga ggccaaggtg gtggacctgg 3661 acaccggcaa gaccctggga gtgaaccagc gcggcgagct gtgtgtgcgc ggccctatga 3721 ttatgtccgg ctacgtgaat aaccctgagg ccacaaacgc cctgatcgac aaggacggct 3781 ggctgcactc tggcgacatt gcctactggg acgaggacga gcacttcttc atcgtggacc 3841 gcctgaagtc tctgatcaag tacaagggct accaggtggc cccagccgag ctggagtcta 3901 tcctgctgca gcaccctaac attttcgacg ccggagtggc cggcctgccc gacgacgatg 3961 ccggcgagct gcctgccgcc gtcgtcgtgc tggaacacgg caagaccatg accgagaagg 4021 agatcgtgga ctatgtggcc agccaggtga caaccgccaa gaagctgcgc ggcggagtgg 4081 tgttcgtgga cgaggtgccc aagggcctga ccggcaagct ggacgcccgc aagatccgcg 4141 agatcctgat caaggctaag aaaggcggca agatcgccgt gtaataattc tagagtcggg 4201 gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa 4261 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg 4321 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc 4381 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta 4441 aaatcgataa ggatccaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 4501 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 4561 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 4621 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 4681 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 4741 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 4801 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 4861 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 4921 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 4981 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 5041 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 5101 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 5161 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 5221 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 5281 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 5341 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 5401 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 5461 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 5521 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 5581 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 5641 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 5701 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 5761 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 5821 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 5881 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 5941 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 6001 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 6061 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 6121 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 6181 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 6241 ccttttgctg gccttttgct cacatggctc gac //