pSh2-GFP vector (V003236)

LOCUS       40924_40062        9120 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSh2-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   Lamont KC, Mudge SR, Liu G, Godwin ID.
  TITLE     Expression patterns of the native Shrunken-2 promoter in Sorghum 
            bicolor visualised through use of the GFP reporter gene
  JOURNAL   Plant Cell Rep. (2017) In press
  PUBMED    28721521
REFERENCE   2  (bases 1 to 9120)
  AUTHORS   Lamont KC, Mudge SR, Godwin ID.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2017) School of Agriculture and Food Sciences, The
            University of Queensland, Mansfield Place, Brisbane, Queensland 
            4072, Australia
REFERENCE   3  (bases 1 to 9120)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9120)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-JUN-2017) School of Agriculture and Food Sciences, The 
            University of Queensland, Mansfield Place, Brisbane, Queensland 
            4072, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      413..2737
                     /regulatory_class="promoter"
     CDS             2747..3463
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      3475..4181
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     regulatory      4196..5604
                     /gene="ScR1MYB1"
                     /regulatory_class="terminator"
     gene            4196..5604
                     /gene="ScR1MYB1"
                     /label=ScR1MYB1
     regulatory      complement(5611..6845)
                     /gene="rice actin"
                     /regulatory_class="terminator"
     gene            complement(5611..6845)
                     /gene="rice actin"
                     /label=rice actin
     primer_bind     complement(6899..6915)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6923..6939)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6947..6977)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6992..7013)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7301..7889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8063..8920)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8921..9025)
                     /label=AmpR promoter