LOCUS       40924_40062        9120 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSh2-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   Lamont KC, Mudge SR, Liu G, Godwin ID.
  TITLE     Expression patterns of the native Shrunken-2 promoter in Sorghum 
            bicolor visualised through use of the GFP reporter gene
  JOURNAL   Plant Cell Rep. (2017) In press
  PUBMED    28721521
REFERENCE   2  (bases 1 to 9120)
  AUTHORS   Lamont KC, Mudge SR, Godwin ID.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2017) School of Agriculture and Food Sciences, The
            University of Queensland, Mansfield Place, Brisbane, Queensland 
            4072, Australia
REFERENCE   3  (bases 1 to 9120)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9120)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-JUN-2017) School of Agriculture and Food Sciences, The 
            University of Queensland, Mansfield Place, Brisbane, Queensland 
            4072, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      413..2737
                     /regulatory_class="promoter"
     CDS             2747..3463
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      3475..4181
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     regulatory      4196..5604
                     /gene="ScR1MYB1"
                     /regulatory_class="terminator"
     gene            4196..5604
                     /gene="ScR1MYB1"
                     /label=ScR1MYB1
     regulatory      complement(5611..6845)
                     /gene="rice actin"
                     /regulatory_class="terminator"
     gene            complement(5611..6845)
                     /gene="rice actin"
                     /label=rice actin
     primer_bind     complement(6899..6915)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6923..6939)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6947..6977)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6992..7013)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7301..7889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8063..8920)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8921..9025)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cggcggcgcg ccacagcata
      421 acttgaagat gacagcagtg cagggataac tgaagcctag gagaaaataa aagggatatg
      481 gacgtttata agaagcatgt attctaacag actacctaat attattcaga ttgtgtgaca
      541 ttctataaaa gacccaaatg cttataaaga tatatcatgt tgtaaaaatt tgatctgcat
      601 ttcctttcgt aagcaataaa acaagtctct tttgggagtt accatgatga aaaagttaaa
      661 atataatctc aataataact ctgcataggg aaaacttatc cttctgctta tgattatttt
      721 ttttttctat tacaagaacc actgtagaat gggaaaatca ctatcttaaa tttaaatcta
      781 caagaacctg gaataattag tgctacattt caatgtctaa tgagaacagt ttcttttgga
      841 cattccaatt ctcctttgaa aatgatatat agttcctgct gtgatataat tcattgtatc
      901 tgtgagtggt accatggcca aggaagaaaa aagagatgca tagaggttct ggaatgactt
      961 acatttagag aatagttaaa aaggctaaaa caaccagtag ggggtggggg gagggggaca
     1021 gaaattttca tgagggacca tgattgaact attgagggtg caaaaaatat tttttttcaa
     1081 atgtggtgaa atacctaaga gatgtgcacc tagcatagtt ttttctttgg gctccccttg
     1141 gcctctcctt tcctcgtccc ctgaagtatc cgattttttt cctggggaag gaaggtgatc
     1201 tgggaaacgg taatcataag agggagagct atgcggcata tgccctcaaa ggcactttgg
     1261 cattatcgaa gcaatatcga tctctgttag tttatcttgc gtgaacgttt gtggaaacta
     1321 ctaccttaca agcattagtg acagctcaga aagttatttc tgaaaggttt catgtgtacc
     1381 gtgggaaatg ttgccaactc aaacaccttc aatatgttgt ttgcacgcaa attcttctgg
     1441 aagaaaggtg tctaaaacta tgagcgggtt acagaaaggt acaaaccacg gctgtgcatt
     1501 ttggaagtat catctataga tgtttgttga ggggaaagcc gcacgccaaa gttatttact
     1561 cagaaacagc ttcaacacac aattgtctgt gatatgatgg catctctcac gcaccatcac
     1621 ctctctctca tgtctgttta ttttattgcc ctttctgctc ataaaaatca ttaagagttt
     1681 ataaatatgc ataggcatac caatatgcac atctattaat tagccagaag atcatcttct
     1741 tattctttag tttagttatt gtttgaaaaa tttgtccagg cctagggagc tcgtgcacag
     1801 tactaatgca tcttcatcaa atgtgaattt caaaaaggaa gtaggaacct atgagagtat
     1861 tgttcaaaat taattaatgg cttctattat gtttatacca aaggccaagg acaaaattgg
     1921 aatgctaatg atggttggct gcgtgagtct gtcgagatac ttgtgagaaa tgtgaacctt
     1981 tgtttccgtg catggacata aaaccaaaag cttccagcct ctttcacggt acttgcactt
     2041 gcaagaaatg agaactcctt ttcatttctg tacgtggacg taacgccaaa gcatccaggc
     2101 tttttcacgg ctgttcatgc ctttacacgg ttcatctcca caagtatgcc tcctcccact
     2161 ctatataagc acatcaacag cacacaatta gacacaggca gcaattgggg ccacaagaac
     2221 acttggggag gcaagtgtga ttttgatctc gcagccacct tcgtgttttg ttgtaagtat
     2281 acattccctt gccatcttta tttgttagct caattcgtaa tcgggaagta ttagtgggta
     2341 gagggcgaga tgctatcatc tatgtagtct gcaaatgcat ccgacattat acaggttgct
     2401 ttgtataatt tgaattgctc cattcttgcc aacattatat tgcaaggtat atgcctagtt
     2461 gcattaaaag ttctgtcttt tgttctaaaa ccattttagc tgcatgcaat ttttgttcac
     2521 ggggataagg agatctgttt cggttacttt gcttgaggag cattctttac gagtccagtt
     2581 ttgtggaagt agtaaatctc ggtttggtca taagacgcca tattgtagcg caaacttata
     2641 ttacttcagt gttcaattta tcgtaaatgt tccttgctgt aaaagaattg cctacttatt
     2701 tatttgaatt gcaggtgtgt ctagtagttg gaggagagcg gccgccatgg tgagcaaggg
     2761 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     2821 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     2881 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
     2941 cacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     3001 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     3061 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     3121 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     3181 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     3241 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     3301 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     3361 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     3421 gaccgccgcc gggatcactc acggcatgga cgagctgtac aagtaattaa ttaactgctt
     3481 taatgagata tgcgagacgc ctatgatcgc atgatatttg ctttcaattc tgttgtgcac
     3541 gttgtaaaaa acctgagcat gtgtagctca gatccttacc gccggtttcg gttcattcta
     3601 atgaatatat cacccgttac tatcgtattt ttatgaataa tattctccgt tcaatttact
     3661 gattgtaccc tactacttat atgtacaata ttaaaatgaa aacaatatat tgtgctgaat
     3721 aggtttatag cgacatctat gatagagcgc cacaataaca aacaattgcg ttttattatt
     3781 acaaatccaa ttttaaaaaa agcggcagaa ccggtcaaac ctaaaagact gattacataa
     3841 atcttattca aatttcaaaa ggccccaggg gctagtatct acgacacacc gagcggcgaa
     3901 ctaataacgt tcactgaagg gaactccggt tccccgccgg cgcgcatggg tgagattcct
     3961 tgaagttgag tattggccgt ccgctctacc gaaagttacg ggcaccattc aacccggtcc
     4021 agcacggcgg ccgggtaacc gacttgctgc cccgagaatt atgcagcatt tttttggtgt
     4081 atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat cgttgggcgg
     4141 gtccagggcg aattttgcga caacatgtcg aggctcagca ggacctgcag agctcggcaa
     4201 cagaagcagc aagaagctat actgtaattt cctagcttga tcaaccacgc cgtctgagaa
     4261 aaaaagcaaa gcaaagagga gcaataaact atgtacctta ttatcatatt tctgttatca
     4321 ccttagcttt atgttgacac aaaggcatgg caaagtagtt gcccgtcata tttcagagcg
     4381 tggaaaaggt aaaagataat attcagtttg cacttgcagt gatcactgac aaggacaggc
     4441 agaatgacct ccactgcaca aaaacatgat catgtcaccc tgtgtagaat accttagccc
     4501 tgcatgcatg catgaagcca atttgtgagc aatcaaaatt agttctacat gttcttgtga
     4561 ctgttaccag ttctgtactg acatataaag atcgatcaat cattgttctc cttggtttgt
     4621 catctctccc tgataaagac tgatcactag ctatacacat ggtacaccta cggacctacc
     4681 gggctgcaga ggtcaggaga tgtaacagtg ttgttatttg atgaatcatc aggcctgtcg
     4741 ttttcagcga gataggatga gatttgggtg aaacatctga aagttccact tcaagtatga
     4801 attaacatgt ggcaggagcg gattacttgc gtaatgaagc aaggaaacat tttcatcgtt
     4861 ggcttgattg atgcgatgta agtggggaca tattcctcct catttgccca gaccatgatc
     4921 aggcccctga cctaataaag caggtttgac actaaacaat ggcgacatca agcaagcatc
     4981 aagcagagga ttaggataga agcattgtat aatatatagt ctgtaaagca gatgtagaat
     5041 atgaaaaaga aagaattagg actggttagt agatttcaca tttagtttgt gcaacctcac
     5101 gttcagatga ttttagatgc atacaaaacg tttacataaa atcgacattc aatcgaccct
     5161 aatacaagct atgggtacaa gtccaccgag actaaatctc aaacaaaaga cgtcaactag
     5221 acgttgcagt aaaatttggt gaagatccca tccacatgct aaaacgtagt cctttctaac
     5281 atccgagtcc ttgataggat cgtagttagg cataccttcg caccactgga atgtgcaggc
     5341 catggtcaac atcaatagtg taatatcctt gaattttata aaattattat gttgactaga
     5401 cggaccaatg gaagaataat ttgaaaccat catccgtata tacgatcagc gtccgtggtt
     5461 gtgtgaagct cgtcaagaca atttcatttg attaataata atccccattc ggtaatgctc
     5521 aggggcgggc gtccgtccga tcaaacgcca ccttcctagg cctgcacggc ccaccaaagc
     5581 tagcatgcct cctcctccta catcgaattc caagcaagat ttccagataa tatgaacagt
     5641 tagtatagta tactctatat agcctggtct gataatatat aaacagccag cctggtgatt
     5701 ggtgtaacaa tttgtgatga catctgcata acttgaaaga tggaaataca taaagtaatt
     5761 ttaaaacaaa ggtcatgtac agattttaaa atcttgtcaa gtcaaaacgt acagaaggcg
     5821 aggaaaaaga gtgatttact ataagcatga tcatcctttt ttttctttca ttttctaaca
     5881 aaattgaagt gctgacacac aacgcgcaca caaaccatag aaagcttgca ctcctcttcc
     5941 actgtgttgt gtacacaaac catagaaagc tttacattcc tcttccattg aaacttaaaa
     6001 aattggcagg atcatgttct ttaacagaat gccacatttt accttggaga taaacagaat
     6061 tgtgctcttt ctacacgcta acatgttgaa ctgaaaacac ttggttgaag acaggcagcc
     6121 aagccacgaa cccacgaaca taggaccccc actgtaccaa atattcttgt gtaaagaaac
     6181 aaaagcttct ctgatccaca tggttccatt tcccctctca atttgtttaa ggaaaagtga
     6241 aaagattatc acttctacag gggatgtcta agctgcgccg tacaactttt aagcggcaaa
     6301 gcgtgctcgt gtgctgctaa agcctgacga tgagaatata tctgatgctg tgatgcatct
     6361 tgcatcatgc tggcacaata gccaagaaag aaaagtatca gaagtgtgtg tgacatgtca
     6421 ttaaaatatc gaacagcaaa ggcaaagtgg aagttacgtg atgtcgaaca tcacaataca
     6481 aactcccatg cactagatca ctgatgcaca agacagaaga aaagaacatt catgcgaaaa
     6541 tactgggatg ataacttcga tgttattcat ttcatagaat aatcgcaact caaaaacaac
     6601 cagaacacgt attttttctg gtaccctcat caggcatctg attaaatttg aataaaccga
     6661 acatatgtgt ccaaaatcac aagtgagaac cacaggtagc aataggtatt acagtctttg
     6721 aatagaagaa aatgataaca gataggccgg ttgaaaactt tgtccacgct aatgaagaaa
     6781 cagcaacaaa aagagagaaa caagcaggag gacggcgata acagctcctc ttggcttagc
     6841 attctcctgc aggcggcgtc gactgcagag gcctgcatgc aagcttggcg taatcatggt
     6901 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6961 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7021 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7081 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7141 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7201 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7261 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7321 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7381 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7441 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7501 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7561 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7621 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7681 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7741 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7801 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7861 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7921 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7981 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8041 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8101 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8161 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8221 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8281 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8341 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8401 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8461 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8521 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8581 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8641 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8701 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8761 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8821 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8881 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8941 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9001 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     9061 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
