pSGNluc vector (V003259)

LOCUS       40924_39947        6683 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSGNluc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Kuri P, Ellwanger K, Kufer TA, Leptin M, Bajoghli B.
  TITLE     A high-sensitivity, bi-directional reporter to monitor NF-kappaB 
            activity in cell culture and zebrafish in real-time
  JOURNAL   J. Cell. Sci. (2016) In press
  PUBMED    27980067
REFERENCE   2  (bases 1 to 6683)
  AUTHORS   Bajoghli B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Directors' Research, EMBL, Meyerhoferstrasse
            1, Heidelberg, BW 69117, Germany
REFERENCE   3  (bases 1 to 6683)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6683)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Cell. 
            Sci. (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Directors' Research, EMBL, Meyerhoferstrasse 1, 
            Heidelberg, BW 69117, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        649..667
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    complement(703..910)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(937..1653)
                     /label=EGFP
                     /note="enhanced GFP"
     5'UTR           complement(1674..1716)
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     promoter        complement(1753..1771)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        complement(1812..1830)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        complement(1840..1858)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1884..1922)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     repeat_region   1945..2067
                     /rpt_family="8x NF-kappaB Binding Sequence"
                     /rpt_unit_seq="gggaattccc"
     promoter        2105..2143
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        2169..2187
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2197..2215
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     5'UTR           2252..2294
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             2343..3992
                     /label=luciferase
                     /note="firefly luciferase"
     3'UTR           4029..4154
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus
                     laevis"
     polyA_signal    complement(4236..4370)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(4474..4492)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4534..4550)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4558..4574)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4582..4612)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4627..4648)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4936..5524)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5698..6555)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6556..6660)
                     /label=AmpR promoter