LOCUS       40924_39947        6683 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSGNluc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Kuri P, Ellwanger K, Kufer TA, Leptin M, Bajoghli B.
  TITLE     A high-sensitivity, bi-directional reporter to monitor NF-kappaB 
            activity in cell culture and zebrafish in real-time
  JOURNAL   J. Cell. Sci. (2016) In press
  PUBMED    27980067
REFERENCE   2  (bases 1 to 6683)
  AUTHORS   Bajoghli B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Directors' Research, EMBL, Meyerhoferstrasse
            1, Heidelberg, BW 69117, Germany
REFERENCE   3  (bases 1 to 6683)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6683)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Cell. 
            Sci. (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Directors' Research, EMBL, Meyerhoferstrasse 1, 
            Heidelberg, BW 69117, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        649..667
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    complement(703..910)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(937..1653)
                     /label=EGFP
                     /note="enhanced GFP"
     5'UTR           complement(1674..1716)
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     promoter        complement(1753..1771)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        complement(1812..1830)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        complement(1840..1858)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1884..1922)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     repeat_region   1945..2067
                     /rpt_family="8x NF-kappaB Binding Sequence"
                     /rpt_unit_seq="gggaattccc"
     promoter        2105..2143
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        2169..2187
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2197..2215
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     5'UTR           2252..2294
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             2343..3992
                     /label=luciferase
                     /note="firefly luciferase"
     3'UTR           4029..4154
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus
                     laevis"
     polyA_signal    complement(4236..4370)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(4474..4492)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4534..4550)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4558..4574)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4582..4612)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4627..4648)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4936..5524)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5698..6555)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6556..6660)
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgtagggat aacagggtaa tgcgcgcgta atacgactca
      661 ctatagggcg aattgggtac ccaccgcggt ggcggcgatc tctccccagc atgcctgcta
      721 ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga
      781 cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg
      841 gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta
      901 gaaggcacag tcgaggctga tcagcgggcc gctttacttg tacagctcgt ccatgccgag
      961 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
     1021 gtctttgctc agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc
     1081 gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat
     1141 gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata
     1201 gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
     1261 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc
     1321 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc
     1381 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa
     1441 gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc
     1501 ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
     1561 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
     1621 cccggtgaac agctcctcgc ccttgctcac catggtggcg accggtggat ctgccaaagt
     1681 tgagcgttta ttctgagctt ctgcaaaaag aacaagcaag ctgatcctgc aaaaagaaca
     1741 agtagcttgt attctatagt gtcacctaaa tcaagctagc tgatcctgca aaaagaacaa
     1801 gtagcttgta ttctatagtg tcacctaaat caagctagcc ctatagtgag tcgtattact
     1861 gcagaatgag ggttcactaa accagctctg cttatatagg cctcccaccg tacacgccta
     1921 cctcgaccct ctagtcgacc tcgacgggaa ttccctctga tgggaattcc cctcgacggg
     1981 aattccctct gatgggaatt cccctcgacg ggaattccct ctgatgggaa ttcccctcga
     2041 cgggaattcc ctctgatggg aattcccctc gagggggggc ccgcgtgagc tcggtacggg
     2101 tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctggtttag tgaaccctca
     2161 ttctgcagta atacgactca ctatagggct agcttgattt aggtgacact atagaataca
     2221 agctacttgt tctttttgca ggatcagctt gcttgttctt tttgcagaag ctcagaataa
     2281 acgctcaact ttggcagatc tatcgataag cttggcattc cggtactgtt ggtaaagcca
     2341 ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg
     2401 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa
     2461 ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt
     2521 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg
     2581 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag
     2641 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca
     2701 tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc
     2761 aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg
     2821 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg
     2881 attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg
     2941 gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct
     3001 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg
     3061 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat
     3121 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt
     3181 acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc
     3241 tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct
     3301 ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat
     3361 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg
     3421 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga
     3481 aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg
     3541 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt
     3601 ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt
     3661 ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc
     3721 aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac
     3781 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg
     3841 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg
     3901 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca
     3961 taaaggccaa gaagggcgga aagatcgccg tgtaattcta gtgactgact aggatctggt
     4021 taccactaaa ccagcctcaa gaacacccga atggagtctc taagctacat aataccaact
     4081 tacactttac aaaatgttgt cccccaaaat gtagccattc gtatctgctc ctaataaaaa
     4141 gaaagtttct tcacattcta aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aacccccccc
     4201 ccccccctgc aggtcgaagt gagtcgtatt acgtagatcc agacatgata agatacattg
     4261 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     4321 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     4381 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaaggtttt ttaattcgcg
     4441 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgcat
     4501 taccctgtta tccctacgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     4561 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     4621 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     4681 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4741 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4801 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4861 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4921 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4981 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     5041 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     5101 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     5161 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     5221 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     5281 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     5341 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     5401 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5461 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5521 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5581 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5641 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5701 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5761 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     5821 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     5881 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     5941 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     6001 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     6061 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     6121 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     6181 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     6241 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     6301 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6361 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6421 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6481 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6541 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6601 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6661 cacatttccc cgaaaagtgc cac
//
