pSGHV0 vector (V003260)

LOCUS       40924_39937        4614 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSGHV0, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Leahy DJ, Dann CE III, Longo P, Perman B, Ramyar KX.
  TITLE     A mammalian expression vector for expression and purification of 
            secreted proteins for structural studies
  JOURNAL   Protein Expr. Purif. 20 (3), 500-506 (2000)
  PUBMED    11087690
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Leahy DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2000) Biophysics, Johns Hopkins University, 725 N.
            Wolfe St., Baltimore, MD 21205, USA
REFERENCE   3  (bases 1 to 4614)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4614)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr. Purif."; date: "2000"; volume: "20"; issue: "3"; pages: 
            "500-506"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-JUL-2000) Biophysics, Johns Hopkins University, 725 N. Wolfe 
            St., Baltimore, MD 21205, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        18..631
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     misc_feature    join(700..709,970..1130,1340..1459,1552..1716,1969..2139)
                     /label=Region: human growth hormone precursor
                     /note="Region: human growth hormone precursor"
     sig_peptide     join(700..709,970..1037)
     misc_feature    2140..2163
                     /label=Region: octahistidine tag
                     /note="Region: octahistidine tag"
     CDS             2170..2193
                     /label=8xHis
                     /note="8xHis affinity tag"
     CDS             2215..2235
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     misc_feature    2233..2284
                     /label=multiple cloning site
                     /note="multiple cloning site"
     polyA_signal    complement(2286..2420)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2747..3335)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3509..4366)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4367..4471)
                     /label=AmpR promoter