LOCUS       40924_39937        4614 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSGHV0, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Leahy DJ, Dann CE III, Longo P, Perman B, Ramyar KX.
  TITLE     A mammalian expression vector for expression and purification of 
            secreted proteins for structural studies
  JOURNAL   Protein Expr. Purif. 20 (3), 500-506 (2000)
  PUBMED    11087690
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Leahy DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2000) Biophysics, Johns Hopkins University, 725 N.
            Wolfe St., Baltimore, MD 21205, USA
REFERENCE   3  (bases 1 to 4614)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4614)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr. Purif."; date: "2000"; volume: "20"; issue: "3"; pages: 
            "500-506"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-JUL-2000) Biophysics, Johns Hopkins University, 725 N. Wolfe 
            St., Baltimore, MD 21205, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        18..631
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     misc_feature    join(700..709,970..1130,1340..1459,1552..1716,1969..2139)
                     /label=Region: human growth hormone precursor
                     /note="Region: human growth hormone precursor"
     sig_peptide     join(700..709,970..1037)
     misc_feature    2140..2163
                     /label=Region: octahistidine tag
                     /note="Region: octahistidine tag"
     CDS             2170..2193
                     /label=8xHis
                     /note="8xHis affinity tag"
     CDS             2215..2235
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     misc_feature    2233..2284
                     /label=multiple cloning site
                     /note="multiple cloning site"
     polyA_signal    complement(2286..2420)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2747..3335)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3509..4366)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4367..4471)
                     /label=AmpR promoter
ORIGIN
        1 aagcttggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
       61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
      301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcctcgatc
      361 gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc
      421 ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct
      481 aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac
      541 ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg
      601 tttcgttttc tgttctgcgc cgttacagat ccaagctcct cgagcaaggc ccaactcccc
      661 gaaccactca gggtcctgtg gacagctcac ctagctgcaa tggctacagg taagcgcccc
      721 taaaatccct ttgggcacaa tgtgtcctga ggggagaggc agcgacctgt agatgggacg
      781 ggggcactaa ccctcaggtt tggggcttct gaatgtgagt atcgccatgt aagcccagta
      841 tttggccaat ctcagaaagc tcctggtccc tggagggatg gagagagaaa aacaaacagc
      901 tcctggagca gggagagtgc tggcctcttg ctctccggct ccctctgttg ccctctggtt
      961 tctccccagg ctcccggacg tccctgctcc tggcttttgg cctgctctgc ctgccctggc
     1021 ttcaagaggg cagtgccttc ccaaccattc ccttatccag gctttttgac aacgctatgc
     1081 tccgcgccca tcgtctgcac cagctggcct ttgacaccta ccaggagttt gtaagctctt
     1141 ggggaatggg tgcgcatcag gggtggcagg aaggggtgac tttcccccgc tgggaaataa
     1201 gaggaggaga ctaaggagct cagggttttt cccgaagcga aaatgcaggc agatgagcac
     1261 acgctgagtg aggttcccag aaaagtaaca atgggagctg gtctccagcg tagaccttgg
     1321 tgggcggtcc ttctcctagg aagaagccta tatcccaaag gaacagaagt attcattcct
     1381 gcagaacccc cagacctccc tctgtttctc agagtctatt ccgacaccct ccaacaggga
     1441 ggaaacacaa cagaaatccg tgagtggatg ccttctcccc aggcggggat gggggagacc
     1501 tgtagtcaga gcccccgggc agcacagcca atgcccgtcc ttcccctgca gaacctagag
     1561 ctgctccgca tctccctgct gctcatccag tcgtggctgg agcccgtgca gttcctcagg
     1621 agtgtcttcg ccaacagcct ggtgtacggc gcctctgaca gcaacgtcta tgacctccta
     1681 aaggacctag aggaaggcat ccaaacgctg atgggggtga gggtggcgcc aggggtcccc
     1741 aatcctggag ccccactgac tttgagagct gtgttagaga aacactgctg ccctcttttt
     1801 agcagtcagg ccctgaccca agagaactca ccttattctt catttcccct cgtgaatcct
     1861 caggcctttc tctacaccct gaaggggagg gaggaaaatg aatgaatgag aaagggaggg
     1921 aacagtaccc aagcgcttgg cctctccttc tcttccttca ctttgcagag gctggaagat
     1981 ggcagccccc ggactgggca gatcttcaag cagacctaca gcaagttcga cacaaactca
     2041 cacaacgatg acgcactact caagaactac gggctgctct actgcttcag gaaggacatg
     2101 gacaaggtcg agacattcct gcgcatcgtg cagtgccgct ctgtggaggg cagctgtggc
     2161 ttcagcggcc accaccacca ccaccaccac cacgactacg acatcccctc ctccgagaac
     2221 ctgtacttcc agggatcctc tagagcgcgc acgcgtgcgg ccgcccgcgg gcatgcttga
     2281 attctgatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     2341 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     2401 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     2461 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgccag ctgcattaat
     2521 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     2581 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2641 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2701 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2761 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2821 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2881 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2941 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3001 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3061 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3121 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3181 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3241 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3301 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3361 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3421 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3481 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3541 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3601 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3661 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3721 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3781 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3841 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3901 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3961 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4021 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4081 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4141 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4201 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4261 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4321 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4381 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4441 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     4501 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     4561 ttcgtcttca agaattggtc gaccaattct catgtttgac agcttatcat cgat
//
