pSGFPS1 vector (V003261)

LOCUS       V003261                 4773 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003261
VERSION     V003261
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Rodriguez MD, Paul Z, Wood CE, Rice KC, Triplett EW.
  TITLE     Construction of Stable Fluorescent Reporter Plasmids for Use in
            Staphylococcus aureus
  JOURNAL   Front Microbiol 8, 2491 (2017)
   PUBMED   29312199
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Rodriguez MD.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2017) Microbiology and Cell Science, University of
            Florida, 1355 Museum Rd., PO Box 110700, Gainesville, FL 32611-0700,
            USA
REFERENCE   3  (bases 1 to 4773)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4773)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front
            Microbiol 8, 2491 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-AUG-2017) Microbiology and Cell Science, University of Florida,
            1355 Museum Rd., PO Box 110700, Gainesville, FL 32611-0700, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..996
                     /codon_start=1
                     /gene="repF"
                     /product="RepF"
                     /label="repF"
                     /protein_id="AUS76872.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
     gene            1..996
                     /gene="repF"
                     /label="repF"
     rep_origin      1179..1567
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     regulatory      1580..1824
                     /label="sarA P1 promoter"
                     /note="sarA P1 promoter"
                     /regulatory_class="promoter"
     CDS             1847..2329
                     /gene="dfrA"
                     /label="Dihydrofolate reductase type 1"
                     /note="Dihydrofolate reductase type 1 from Tn4003 from
                     Staphylococcus aureus. Accession#: P13955"
     CDS             2349..3059
                     /label="superfolder GFP"
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
     regulatory      3459..3516
                     /label="atl transcription terminator"
                     /note="atl transcription terminator"
                     /regulatory_class="terminator"
     rep_origin      3913..4090
                     /label="sso"
                     /note="sso"
     CDS             complement(4351..4362)
                     /label="WELQut site"
                     /note="WELQut protease recognition and cleavage site"
     misc_feature    4559..4579
                     /label="dso"
                     /note="dso"