LOCUS       V003261                 4773 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003261
VERSION     V003261
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Rodriguez MD, Paul Z, Wood CE, Rice KC, Triplett EW.
  TITLE     Construction of Stable Fluorescent Reporter Plasmids for Use in
            Staphylococcus aureus
  JOURNAL   Front Microbiol 8, 2491 (2017)
   PUBMED   29312199
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Rodriguez MD.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2017) Microbiology and Cell Science, University of
            Florida, 1355 Museum Rd., PO Box 110700, Gainesville, FL 32611-0700,
            USA
REFERENCE   3  (bases 1 to 4773)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4773)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front
            Microbiol 8, 2491 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-AUG-2017) Microbiology and Cell Science, University of Florida,
            1355 Museum Rd., PO Box 110700, Gainesville, FL 32611-0700, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..996
                     /codon_start=1
                     /gene="repF"
                     /product="RepF"
                     /label="repF"
                     /protein_id="AUS76872.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
     gene            1..996
                     /gene="repF"
                     /label="repF"
     rep_origin      1179..1567
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     regulatory      1580..1824
                     /label="sarA P1 promoter"
                     /note="sarA P1 promoter"
                     /regulatory_class="promoter"
     CDS             1847..2329
                     /gene="dfrA"
                     /label="Dihydrofolate reductase type 1"
                     /note="Dihydrofolate reductase type 1 from Tn4003 from
                     Staphylococcus aureus. Accession#: P13955"
     CDS             2349..3059
                     /label="superfolder GFP"
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
     regulatory      3459..3516
                     /label="atl transcription terminator"
                     /note="atl transcription terminator"
                     /regulatory_class="terminator"
     rep_origin      3913..4090
                     /label="sso"
                     /note="sso"
     CDS             complement(4351..4362)
                     /label="WELQut site"
                     /note="WELQut protease recognition and cleavage site"
     misc_feature    4559..4579
                     /label="dso"
                     /note="dso"
ORIGIN
        1 atgcaatata atactactag aagtataacc gaaaatcaag ataataaaac gttaaaagat
       61 atgacgaaaa gtgggaaaca acgcccatgg agagaaaaga aaatagataa tgtaagctat
      121 gcagatatac tagaaatttt aaaaatcaaa aaggctttta atgtaaaaca atgtggtaat
      181 attttagaat ttaagccaac tgatgaaggc tatttgaagt tacataagac atggttttgt
      241 aaatcaaaat tatgtccggt ttgtaattgg agacgtgcta tgaaaaatag ttatcaagct
      301 caaaaagtga ttgaaaaagt aattaaggaa aagccaaaag cacgttggtt gtttttaaca
      361 ctttcaacaa aaaatgcgat agatggagat actttagaac aaagtttgaa gcatctaact
      421 aaagcatttg ataggttgag tagatataaa aaggttaaac aaaatcttgt tggatttatg
      481 cgttcaacag aagttaccgt taataaaaat gacggtagtt ataatcagca catgcatgtt
      541 ttgttatgtg ttgaaaatgc atattttaga aaaaaagaga attatataac tcaagaagaa
      601 tgggttaatt tatggcaaag agcattacaa gttgactatc gacctgttgc taatgttaaa
      661 gcgatcaaac cgaatagaaa aggcgataaa gacattgaat cggcaatcaa agagacctca
      721 aaatattcgg ttaaatcatc tgatttttta actgatgatg atgaaaaaaa tcaagaaatt
      781 gtaagtgatt tagaaaaagg tttgtatcga aaacgtatgt taagttatgg tggattgctt
      841 aaacaaaaac ataaaatttt aaacttagac gatgtcgaag atggtaattt gattaatgca
      901 agtgatgaag ataaaacaac agacgaagaa gaaaaagcac attcaattac cgcaatttgg
      961 aatttcgaaa agcaaaatta ttatttaaga cattagtgtt gactaatgtc ttttttgttg
     1021 attttttata aaaaagtact gtcttatttt tgtgacaaat gctgtatgta gtgtcacaaa
     1081 aataagacaa acgcaatata ttgtgtcaca aaaataagac agtacagctt tgtatgatcc
     1141 gtcgacgaaa gcctggccac gatgcgtccg gcgtagagga tctgaagatc agcagttcaa
     1201 cctgttgata gtacgtacta agctctcatg tttcacgtac taagctctca tgtttaacgt
     1261 actaagctct catgtttaac gaactaaacc ctcatggcta acgtactaag ctctcatggc
     1321 taacgtacta agctctcatg tttcacgtac taagctctca tgtttgaaca ataaaattaa
     1381 tataaatcag caacttaaat agcctctaag gttttaagtt ttataagaaa aaaaagaata
     1441 tataaggctt ttaaagcttt taaggtttaa cggttgtgga caacaagcca gggatgtaac
     1501 gcactgagaa gcccttagag cctctcaaag caattttgag tgacacagga acacttaacg
     1561 gctgacatgg gaattcgagc tgatattttt gactaaacca aatgctaacc cagaaataca
     1621 atcactgtgt ctaatgaata atttgtttta taaacacttt tttgtttact tctcattttt
     1681 aattagttat aattaactaa ataatagagc attaaatata tttaataaaa cttatttaat
     1741 gcaaaattat gactaacata tctataataa ataaagatta gatatcaata tattatcggg
     1801 caaatgtatc gagcaagatg catcggatcg atccaggagg tataccatga cattatcaat
     1861 aattgtcgct cacgataaac aaagagtcat tgggtaccaa aatcaattac cttggcactt
     1921 accaaatgat ttaaagcata ttaaacaact gaccactggg aatacacttg taatggcacg
     1981 gaaaactttt aattctatag ggaagccatt gccaaataga cgtaacgtcg tactcactaa
     2041 ccaagcttca tttcgccatg aaggggtaga tgttataaac tctcttgatg aaattaaaga
     2101 gttatctggt catgttttta tatttggagg acaaacgtta tacgaagcaa tgattgacca
     2161 ggtagatgat atgtatatca cagtaataga tggaaagttt caaggagaca cattctttcc
     2221 accatacaca ttcgaaaact gggaagtcga atcttcagta gaaggtcaac tagatgaaaa
     2281 aaatactata ccgcatacat tcttacattt agtgcgtaga aaagggaaat aggcgcgcca
     2341 tgcccgggag caaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
     2401 atggtgatgt taatgggcac aaattttctg tccgtggaga gggtgaaggt gatgctacaa
     2461 acggaaaact cacccttaaa tttatttgca ctactggaaa actacctgtt ccgtggccaa
     2521 cacttgtcac tactctgacc tatggtgttc aatgcttttc ccgttatccg gatcacatga
     2581 aacgtcatga ctttttcaag agtgccatgc ctgaaggtta tgtacaggaa cgcactatat
     2641 ctttcaaaga tgacgggacc tacaagacgc gtgctgaagt caagtttgaa ggtgataccc
     2701 ttgttaatcg tatcgagtta aagggtattg attttaaaga agatggaaac attcttggac
     2761 acaaactcga gtacaacttt aactcacaca atgtatacat cacggcagac aaacaaaaga
     2821 atggaatcaa agctaacttc aaaattcgcc acaacgttga agatggttcc gttcaactag
     2881 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
     2941 attacctgtc gacacaatct gtcctttcga aagatcctaa cgaaaagcgt gaccacatgg
     3001 tccttcttga gtttgtaact gctgctggga ttacacatgg catggatgag ctctacaaat
     3061 aataggcgcg cctattctaa tgcataataa atactgataa catcttatat tttgtattat
     3121 attttgtatt atcgttgaca tgtataattt tgatatcaaa aactgatttt ccctctatta
     3181 ttttcgagat ttattttctt aattctcttt aacaaactag aaatattgta tatacaaaaa
     3241 attataaata atagatgaat agtttaatta taggtgttca tcaatcgaaa aagcaacgta
     3301 tcttatttaa agtgcgttgc ttttttctca tttataaggt taaataattc tcatatatca
     3361 agcaaagtga caggcgatgc ggccgctagc ctaggagctc ggtacccggg gatccgaatc
     3421 atgaattaca agcaaaagta gcggtgattg ttaaaattga tggtaaacaa tcaccgctat
     3481 ttttgcttgt gtatgtataa aaaagggatc aaaggtcatc ccccatgatt gatagtgggg
     3541 ggatgacttt tgatcctatg ttcatgttgc ttatttaatc gcctttgatc actttaaaat
     3601 accttaaaac ccctggaatt tctggctttg ccagacctat catttttgaa tgatagcaaa
     3661 ttctccttat gctcttacgg agtttttaga gaaaaattaa aaattctcga tttttgataa
     3721 aaaacgccct gcaggaattt agaaaaacat gtggaagttt taaaggattt tatgctaatt
     3781 tttaatttgc atgtaactcg aggggaatat ttgaggggat tttgaaacga gtttcttctt
     3841 gttttcacac tgttttttta ttcctattgg tgttgttgct tactttttgt ttttctataa
     3901 agatgatgct tttgacgaga tggaagaaaa atatgattat catgaagata ataaaaaata
     3961 gacgacgcat ttatgccgag aaaatttatt gatgttgaga agaaccctta actaaacttg
     4021 cagacgaatg tcggcatagc gtgagctatt aagccgacca ttcgacaagt tttgggattg
     4081 ttaagggttc cgaggctcaa cgtcaataaa gcaattggaa taaatagtat ctaaagtaac
     4141 ccgtatttaa gattacattg tacatccttc gaaaccatag tagctgcgat tccagctagg
     4201 tttcctttta tcccaatttt agctaatttt tttgctacgc cctttaagtt tttggcttta
     4261 acatcttcaa aaactgctgt gattatgttt ccagttaaaa gttctccata tgagtttaaa
     4321 acttcagaat agaaacagtc accgctattt ttgtagttcc caccaactgc tctaggtgat
     4381 attgaactag ttttcttttt attctctatg tcgtttttta gtttttggaa tccttcagga
     4441 acatatccat atcgagattt ggccgatttt atttgataat ccttttgact ttttagaaag
     4501 ttttatttat tcattggttt tcacattaat ttatttaggt ttagagggtc ataaaaagaa
     4561 gaaggaatag gttgtttttt gaaacgagtg tgaacgagtt tcttcttgtc ttgatactat
     4621 atagaaataa ctcgatttta tatatatagc tgtaactgtt gatattacag tgtttaaacg
     4681 tgtttttgtg cgtgaaagga aaatttgaca ataaaaaacc ccagttatat tattaaggtg
     4741 tcgaatctta aataatactg ggggtctttt tat
//
