pSEmp18BBXM vector (V003387)

LOCUS       V003387                 7382 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003387
VERSION     V003387
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7382)
  AUTHORS   Nguyen TN, Gourdon M-H., Hansson M, Robert A, Samuelson P, Libon C,
            Andre'oni C, Nygren P-A., Stahl S.
  TITLE     Hydrophobicity engineering to facilitate surface display of
            heterologous gene products on Staphylococcus xylosus
  JOURNAL   J. Biotechnol. (1997) In press
REFERENCE   2  (bases 1 to 7382)
  AUTHORS   Liljeqvist S, Samuelson P, Hansson M, Nguyen TN, Binz H, Stahl S.
  TITLE     Surface display of the cholera toxin B subunit on Staphylococcus
            xylosus and Staphylococcus carnosus
  JOURNAL   Appl. Environ. Microbiol. 63 (7), 2481-2488 (1997)
   PUBMED   9212399
REFERENCE   3  (bases 1 to 7382)
  AUTHORS   Samuelson PN.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-1995) Biochemistry and Biotechnology, KTH, Royal
            Institute of Technology, Teknikringen 34, Stockholm, 10044, Sweden
REFERENCE   4  (bases 1 to 7382)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7382)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Biotechnol. (1997) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "1997"; volume: "63"; issue: "7"; pages:
            "2481-2488"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (17-OCT-1995) Biochemistry and Biotechnology, KTH, Royal Institute
            of Technology, Teknikringen 34, Stockholm, 10044, Sweden"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label="AmpR promoter"
     CDS             201..1058
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2284..2298)
                     /label="enterokinase site"
                     /note="enterokinase recognition and cleavage site"
     CDS             3787..4434
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     protein_bind    5018..5039
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5054..5084
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5092..5108
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5116..5132
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     sig_peptide     5339..5446
     misc_feature    5473..5529
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"