LOCUS       V003387                 7382 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003387
VERSION     V003387
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7382)
  AUTHORS   Nguyen TN, Gourdon M-H., Hansson M, Robert A, Samuelson P, Libon C,
            Andre'oni C, Nygren P-A., Stahl S.
  TITLE     Hydrophobicity engineering to facilitate surface display of
            heterologous gene products on Staphylococcus xylosus
  JOURNAL   J. Biotechnol. (1997) In press
REFERENCE   2  (bases 1 to 7382)
  AUTHORS   Liljeqvist S, Samuelson P, Hansson M, Nguyen TN, Binz H, Stahl S.
  TITLE     Surface display of the cholera toxin B subunit on Staphylococcus
            xylosus and Staphylococcus carnosus
  JOURNAL   Appl. Environ. Microbiol. 63 (7), 2481-2488 (1997)
   PUBMED   9212399
REFERENCE   3  (bases 1 to 7382)
  AUTHORS   Samuelson PN.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-1995) Biochemistry and Biotechnology, KTH, Royal
            Institute of Technology, Teknikringen 34, Stockholm, 10044, Sweden
REFERENCE   4  (bases 1 to 7382)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7382)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Biotechnol. (1997) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "1997"; volume: "63"; issue: "7"; pages:
            "2481-2488"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (17-OCT-1995) Biochemistry and Biotechnology, KTH, Royal Institute
            of Technology, Teknikringen 34, Stockholm, 10044, Sweden"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label="AmpR promoter"
     CDS             201..1058
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2284..2298)
                     /label="enterokinase site"
                     /note="enterokinase recognition and cleavage site"
     CDS             3787..4434
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     protein_bind    5018..5039
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5054..5084
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5092..5108
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5116..5132
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     sig_peptide     5339..5446
     misc_feature    5473..5529
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 gagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagagct tttaaaaagc aaatatgagc caaataaata tattctaatt
     2101 ctacaaacaa aaatttgagc aaattcagtg tcgatttttt aagacactgc ccagttacat
     2161 gcaaattaaa attttcatga ttttttatag ttcctaacag ggttaaaatt tgtataacga
     2221 aagtataatg tttatataac gttagtataa taaagcattt taacattata cttttgataa
     2281 tcgtttatcg tcgtcatcac aataactttt aaaatactcg tgcataattc acgctgacct
     2341 cccaataact acatggtgtt atcgggaggt cagctgttag cacttatatt ttgttattgt
     2401 tcttcctcga tttcgtctat cattttgtga ttaatttctc ttttttcttg ttctgttaag
     2461 tcataaagtt cactagctaa atactctttt tgtttccaaa tataaaaaat ttgatagata
     2521 tattacggtt ggatcaattt cttttaagta atctaaatcc ccatttttta atttcttttt
     2581 agcctcttta aataatcctg aataaactaa tacctgttta cctttaagtg atttataaaa
     2641 tgcatcaaag actttttgat ttattaaata atcactatct ttaccagaat acttagccat
     2701 ttcatataat tctttattat tattttgtct tattttttga acttgaactt gtgttatttc
     2761 tgaaatgccc gttacatcac gccataaatc taaccattct tgttggctaa tataatatct
     2821 tttatctgtg aaatacgatt tatttactgc aattaacaca tgaaaatgag gattataatc
     2881 atctcttttt ttattatatg taatctctaa cttacgaaca tatcccttta taacactacc
     2941 tacttttttt ctctttataa gttttctaaa agaattatta taacgtttta tttcattttc
     3001 taattcatca ctcattacat taggtgtagt caaagttaaa aagataaact cctttttctc
     3061 ttgctgctta atatattgca tcactaaaga taaacccaat gcatcttttc tagcttttct
     3121 ccaagcacag acaggacaaa atcgattttt acaagaatta gctttatata atttctgttt
     3181 ttctaaagtt ttatcagcta caaaagacag aaatgtattg caatcttcaa ctaaatccat
     3241 ttgattctct ccaatatgac gtttaataaa tttctgaaat acttgatttc tttgtttttt
     3301 ctcagtatac ttttccatgt tataacacat aaaaacaact tagttttcac aaactatgac
     3361 aataaaaaaa gttgcttttt cccctttcta tgtatgtttt ttactagtca tttaaaacga
     3421 tacattaata ggtacgaaaa agcaatcccc cccgcgctta aaaccagtca taccaataac
     3481 ttaagggtaa ctagcctcgc cggcaatagt tacccttatt atcaagataa gaaagaaaag
     3541 gatttttcgc tacgctcaaa tcctttaaaa aaacacaaaa gaccacattt tttaatgtgg
     3601 tctttattct tcaactaaag cacccattag ttcaacaaac gaaaattgga taaagtggga
     3661 tatttttaaa atatatattt atgttacagt aatattgact tttaaaaaag gattgattct
     3721 aatgaagaaa gcagacaagt aagcctccta aattcacttt agataaaaat ttaggaggca
     3781 tatcaaatga actttaataa aattgattta gacaattgga agagaaaaga gatatttaat
     3841 cattatttga accaacaaac gacttttagt ataaccacag aaattgatat tagtgtttta
     3901 taccgaaaca taaaacaaga aggatataaa ttttaccctg catttatttt cttagtgaca
     3961 agggtgataa actcaaatac agcttttaga actggttaca atagcgacgg agagttaggt
     4021 tattgggata agttagagcc actttataca atttttgatg gtgtatctaa aacattctct
     4081 ggtatttgga ctcctgtaaa gaatgacttc aaagagtttt atgatttata cctttctgat
     4141 gtagagaaat ataatggttc ggggaaattg tttcccaaaa cacctatacc tgaaaatgct
     4201 ttttctcttt ctattattcc atggacttca tttactgggt ttaacttaaa tatcaataat
     4261 aatagtaatt accttctacc cattattaca gcaggaaaat tcattaataa aggtaattca
     4321 atatatttac cgctatcttt acaggtacat cattctgttt gtgatggtta tcatgcagga
     4381 ttgtttatga actctattca ggaattgtca gataggccta atgactggct tttataatat
     4441 gagataatgc cgactgtact ttttacagtc ggttttctaa tgtcactaac ctgccccgtt
     4501 agttgaagaa ggtttttata ttacagctcc agatccatat ccttcttttt ctgaaccgac
     4561 ttctcctttt tcgcttcttt attccaattg ctttattgac gttgagcctc ggaaccctta
     4621 acaatcccaa aacttgtcga atggtcggct taatagctca cgctatgccg acattcgtct
     4681 gcaagtttag ttaagggttc ttctcaacgc acaataaatt ttctcggcat aaatgcgtgg
     4741 tctaattttt atttttaata accttgatag caaaaaatgc cattccaata caaaaccaca
     4801 tacctataat cgataaccac ataacagtca taaaaccact cctttttaac aaactttatc
     4861 acaagaaata tttaaatttt aaatgccttt attttgaatt ttaaggggca ttttaaagat
     4921 ttaggggtaa atcatatagt tttatgccta aaaacctaca gaagctctgg cacgacaggt
     4981 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttac ctcactcatt
     5041 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     5101 gataacaatt tcacacagga aacagctatg accatgatta cgaattagcg gccgctcgaa
     5161 atagcgtgat tttgcggttt taagcctttt acttcctgaa taaatctttc agcaaaatat
     5221 ttattttata agttgtaaaa cttaccttta aatttaatta taaatataga ttttagtatt
     5281 gcaatacata attcgttata ttatgatgac tttacaaata catacagggg gtattaattt
     5341 gaaaaagaaa aacatttatt caattcgtaa actaggtgta ggtattgcat ctgtaacttt
     5401 aggtacatta cttatatctg gtggcgtaac acctgctgca aatgctgcgc aacacgatga
     5461 agccgtagac gcgaattcga gctcggtacc cggggatcct ctagagtcga cctgcaggca
     5521 tgcaagctta gatgctctag caaaagccaa agcagatgcc cttaaagaat tcaacaaata
     5581 tggagtaagt gactattaca agaatctaat caacaatgcc aaaactgttg aaggcgtaaa
     5641 agaccttcaa gcacaagttg ttgaatcagc gaagaaagcg cgtatttcag aagcaacaga
     5701 tggcttatct gatttcttga aatcacaaac acctgctgaa gatactgtta aatcaattga
     5761 attagctgaa gctaaagtct tagctaacag agaacttgac aaatatggag taagtgacta
     5821 tcacaagaac ctaatcaaca atgccaaaac tgttgaaggt gtaaaagacc ttcaagcaca
     5881 agttgttgaa tcagcgaaga aagcgcgtat ttcagaagca acagatggct tatctgattt
     5941 cttgaaatca caaacacctg ctgaagatac tgttaaatca attgaattag ctgaagctaa
     6001 agtcttagct aacagagaac ttgacaaata tggagtaagt gactattaca agaacctaat
     6061 caacaatgcc aaaactgttg aaggtgtaaa agcactgata gatgaaattt tagctgcatt
     6121 acctctcgag agcttcgctc aagcaccaaa agaggaagac aataacaagc ctggcaaaga
     6181 agacaataac aagcctggca aagaagacaa taacaagcct ggcaaagaag acaacaacaa
     6241 gcctggcaaa gaagacaaca acaagcctgg taaagaagac aacaacaagc ctggcaaaga
     6301 agacggcaac aagcctggta aagaagacaa caaaaaacct ggtaaagaag atggcaacaa
     6361 gcctggtaaa gaagacaaca aaaaacctgg taaagaagac ggcaacaagc ctggcaaaga
     6421 agatggcaac aaacctggta aagaagatgg taacggagta catgtcgtta aacctggtga
     6481 tacagtaaat gacattgcaa aagcaaacgg cactactgct gacaaaattg ctgcagataa
     6541 caaattagct gataaaaaca tgatcaaacc tggtcaagaa cttgttgttg ataagaagca
     6601 accagcaaac catgcagatg ctaacaaagc tcaagcatta ccagaaactg gcgaagaaaa
     6661 tccattcatc ggtacaactg tatttggtgg attatcatta gccttaggtg cagcgttatt
     6721 agctggacgt cgtcgcgaac tataaaaaca aacaatacac aacgatagat atcatcgatt
     6781 ctcttgtttg ctccagactc tcaggcaatg acctgatagc ctttgtagac ctctcaaaaa
     6841 tagctaccct ctccggcatg aatttatcag ctagaacggt tgaatatcat attgatggtg
     6901 atttgactgt ctccggcctt tctcacccgt ttgaatcttt acctacacat tactcaggca
     6961 ttgcatttaa aatatatgag ggttctaaaa atttttatcc ttgcgttgaa ataaaggctt
     7021 ctcccgcaaa agtattacag ggtcataatg tttttggtac aaccgattta gctttatgct
     7081 ctgaggcttt attgcttaat tttgctaatt ctttgccttg cctgtatgat ttattggatg
     7141 ttggaatttg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     7201 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     7261 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     7321 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     7381 ga
//
