pSCCmec vector (V003415)

LOCUS       V003415                11172 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003415
VERSION     V003415
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11172)
  AUTHORS   Wu Z.
  TITLE     Functional analyses of CcrC2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11172)
  AUTHORS   Wu Z.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11172)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11172)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (07-MAR-2017) College of Animal Science "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(4..144)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             1313..1912
                     /codon_start=1
                     /product="RepF"
                     /label="RepF"
                     /note="temperature-sensitive replicon from pE194"
                     /protein_id="ASN64784.1"
                     /translation="MSENVIKETENKKNSRGRNWTFVLYPESAKAEWLEYLKELHIQFV
                     VSPLHDRDTDTEDRMKKEHYHILVMYEGNKSYEQIKIITEELNATIPQIAGSVKGLVRY
                     MLHMDDPNKFKYQKEDMIVYGGVDVDELLKKTTTDRYKLIKEMIEFIDEQGIVEFKSLM
                     DYAMKFKFDDWFPLLCDNSAYVIQEYIKSNRYKSDR"
     CDS             3263..3910
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     regulatory      4575..4673
                     /label="PmecA"
                     /note="PmecA"
                     /regulatory_class="promoter"
     CDS             4671..6431
                     /codon_start=1
                     /product="N-terminal tagged CcrC2"
                     /label="N-terminal tagged CcrC2"
                     /protein_id="ASN64786.1"
                     /translation="MGFFSKITDKQKHTINKTKKGAGATMKRKVAIYTRVSTKEQLKQG
                     YSIQQQADLLIKKAKQIFPDDEYEIYSDEGISGKNIEARPGMKRLLEDIENRQIKVVMS
                     WKLNRLSRSNRDIQNIIYEFRRGGAYYISISENIDTSTQNGEMMIGAFGLVAQIERETI
                     VSNVKMGMNAKAKQGEAITGRVLGYVLKPNPLTGKNELVIDEFEANIVRQIFDLYLNQN
                     KGLKAIANYLNKQGYHTINKKPFSVYGVKYILNNPVYKGYVRFNNYQNWAVERRSGKND
                     KSKVILVQGKHKAIIDEEIFDKTHEKLAAKSFKPGRPIGGDFWLRGLIKCPECGNNMVC
                     RRTYYNTKKSNERTIKRYYICSLFNRAGSAACHSNAIKAEVVERVVYFHLRRILSQPHV
                     VKTIATQVIDAMEKKKANQTPMSIDTNSLENQKKKLQKRKERLIDLYLDEEIDKETMQN
                     KQDKLNVQLEKIDEQLKQAEVMNKASQDIAIPNYKKIKGQLYVMLYRFTGYMKKANPEA
                     KNKLMHMLIDSIELTTDKQVKLIKYKIDESLLPQSIKKDCGNFFMPKFNFVINVTKKNR
                     IENLSLLPLF"
     repeat_region   4685..4723
                     /label="attSCC"
                     /note="attSCC"
     CDS             6928..8934
                     /codon_start=1
                     /gene="mecA"
                     /product="PBP2a"
                     /label="mecA"
                     /protein_id="ASN64787.1"
                     /translation="MKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFKQV
                     YKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKIKTN
                     YGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNNVELA
                     NTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTVKKMDE
                     YLRDFAKKFHLTTNETESRNYPLEKATSHLLGYVGPINSEELKQKEYKGYKDDAVIGKK
                     GLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQKSIYNNM
                     KNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPLLNKFQITT
                     SPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVVNGNIDLKQA
                     IESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKNLDNEILLADS
                     GYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISKENINLLTDGMQ
                     QVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKDNPNMMMAINVKD
                     VQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            6928..8934
                     /gene="mecA"
                     /label="mecA"
     promoter        complement(8976..8994)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9001..9017)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      9054..9140
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        9266..9370
                     /label="AmpR promoter"
     CDS             9371..10228
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      10402..10990
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"