LOCUS       V003415                11172 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003415
VERSION     V003415
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11172)
  AUTHORS   Wu Z.
  TITLE     Functional analyses of CcrC2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11172)
  AUTHORS   Wu Z.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11172)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11172)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (07-MAR-2017) College of Animal Science "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(4..144)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             1313..1912
                     /codon_start=1
                     /product="RepF"
                     /label="RepF"
                     /note="temperature-sensitive replicon from pE194"
                     /protein_id="ASN64784.1"
                     /translation="MSENVIKETENKKNSRGRNWTFVLYPESAKAEWLEYLKELHIQFV
                     VSPLHDRDTDTEDRMKKEHYHILVMYEGNKSYEQIKIITEELNATIPQIAGSVKGLVRY
                     MLHMDDPNKFKYQKEDMIVYGGVDVDELLKKTTTDRYKLIKEMIEFIDEQGIVEFKSLM
                     DYAMKFKFDDWFPLLCDNSAYVIQEYIKSNRYKSDR"
     CDS             3263..3910
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     regulatory      4575..4673
                     /label="PmecA"
                     /note="PmecA"
                     /regulatory_class="promoter"
     CDS             4671..6431
                     /codon_start=1
                     /product="N-terminal tagged CcrC2"
                     /label="N-terminal tagged CcrC2"
                     /protein_id="ASN64786.1"
                     /translation="MGFFSKITDKQKHTINKTKKGAGATMKRKVAIYTRVSTKEQLKQG
                     YSIQQQADLLIKKAKQIFPDDEYEIYSDEGISGKNIEARPGMKRLLEDIENRQIKVVMS
                     WKLNRLSRSNRDIQNIIYEFRRGGAYYISISENIDTSTQNGEMMIGAFGLVAQIERETI
                     VSNVKMGMNAKAKQGEAITGRVLGYVLKPNPLTGKNELVIDEFEANIVRQIFDLYLNQN
                     KGLKAIANYLNKQGYHTINKKPFSVYGVKYILNNPVYKGYVRFNNYQNWAVERRSGKND
                     KSKVILVQGKHKAIIDEEIFDKTHEKLAAKSFKPGRPIGGDFWLRGLIKCPECGNNMVC
                     RRTYYNTKKSNERTIKRYYICSLFNRAGSAACHSNAIKAEVVERVVYFHLRRILSQPHV
                     VKTIATQVIDAMEKKKANQTPMSIDTNSLENQKKKLQKRKERLIDLYLDEEIDKETMQN
                     KQDKLNVQLEKIDEQLKQAEVMNKASQDIAIPNYKKIKGQLYVMLYRFTGYMKKANPEA
                     KNKLMHMLIDSIELTTDKQVKLIKYKIDESLLPQSIKKDCGNFFMPKFNFVINVTKKNR
                     IENLSLLPLF"
     repeat_region   4685..4723
                     /label="attSCC"
                     /note="attSCC"
     CDS             6928..8934
                     /codon_start=1
                     /gene="mecA"
                     /product="PBP2a"
                     /label="mecA"
                     /protein_id="ASN64787.1"
                     /translation="MKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFKQV
                     YKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKIKTN
                     YGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNNVELA
                     NTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTVKKMDE
                     YLRDFAKKFHLTTNETESRNYPLEKATSHLLGYVGPINSEELKQKEYKGYKDDAVIGKK
                     GLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQKSIYNNM
                     KNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPLLNKFQITT
                     SPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVVNGNIDLKQA
                     IESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKNLDNEILLADS
                     GYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISKENINLLTDGMQ
                     QVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKDNPNMMMAINVKD
                     VQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            6928..8934
                     /gene="mecA"
                     /label="mecA"
     promoter        complement(8976..8994)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9001..9017)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      9054..9140
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        9266..9370
                     /label="AmpR promoter"
     CDS             9371..10228
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      10402..10990
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
       61 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
      121 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
      181 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
      241 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcaggaacg gattgttgat
      301 gattacgaaa atattaagag cacagactat tacacagaaa atcaagaatt aaaaaaacgt
      361 agagagagtt tgaaagaagt agtgaataca tggaaagagg ggtatcacga aaaaagtaaa
      421 gaggttaata aattaaagcg agagaatgat agtttgaatg agcagttgaa tgtatcagag
      481 aaatttcaag atagtacagt gactttatat cgtgctgcga gggcgaattt ccctgggttt
      541 gagaaagggt ttaataggct taaagagaaa ttctttaatg attccaaatt cgagcgtgtg
      601 ggacagttta tggatgttgt acaggataat gtccagaagg tcgatagaaa gcgtgagaaa
      661 cagcgtacag acgatttaga gatgtagagg tacttttatg ccgagaaaac tttttgcgtg
      721 tgacagtcct taaaatatac ttagagcgta agcgaaagta gtagcgacag ctattaactt
      781 tcggttgcaa agctctagga tttttaatgg acgcagcgca tcacacgcaa aaaggaaatt
      841 ggaataaatg cgaaatttga gatgttaatt aaagaccttt ttgaggtctt tttttcttag
      901 atttttgggg ttatttaggg gagaaaacat aggggggtac tacgacctcc cccctaggtg
      961 tccattgtcc attgtccaaa caaataaata aatattgggt ttttaatgtt aaaaggttgt
     1021 tttttatgtt aaagtgaaaa aaacagatgt tgggaggtac agtgatggtt gtagatagaa
     1081 aagaagagaa aaaagttgct gttactttaa gacttacaac agaagaaaat gagatattaa
     1141 atagaatcaa agaaaaatat aatattagca aatcagatgc aaccggtatt ctaataaaaa
     1201 aatatgcaaa ggaggaatac ggtgcatttt aaacaaaaaa agatagacag cactggcatg
     1261 ctgcctatct atgactaaat tttgttaaat gtattagcac cgttattata tcatgagcga
     1321 aaatgtaata aaagaaactg aaaacaagaa aaattcaaga ggacgtaatt ggacatttgt
     1381 tttatatcca gaatcagcaa aagccgagtg gttagagtat ttaaaagagt tacacattca
     1441 atttgtagtg tctccattac atgataggga tactgataca gaagatagga tgaaaaaaga
     1501 gcattatcat attctagtga tgtatgaggg taataaatct tatgaacaga taaaaataat
     1561 tacagaagaa ttgaatgcga ctattccgca gattgcagga agtgtgaaag gtcttgtgag
     1621 atatatgctt cacatggacg atcctaataa atttaaatat caaaaagaag atatgatagt
     1681 ttatggcggt gtagatgttg atgaattatt aaagaaaaca acaacagata gatataaatt
     1741 aattaaagaa atgattgagt ttattgatga acaaggaatc gtagaattta agagtttaat
     1801 ggattatgca atgaagttta aatttgatga ttggttcccg cttttatgtg ataactcggc
     1861 gtatgttatt caagaatata taaaatcaaa tcggtataaa tctgaccgat agattttgaa
     1921 tttaagagtg tcacaagaca ctcttttttc gcaccagcga aaactggttt aagccgactg
     1981 cgcaaaagac ataatcgatt cacaaaaaat aggcacacga aaaacaagtt aagggatgca
     2041 gtttatgcat cccttaactt acttattaaa taatttatag ctattgaaaa gagataagaa
     2101 ttgttcaaag ctaatattgt ttaaatcgtc aattcctgca tgttttaagg aattgttaaa
     2161 ttgatttttt gtaaatattt tcttgtattc tttgttaacc catttcataa cgaaataatt
     2221 atacttttgt ttatctttgt gtgatattct tgattttttt ctacttaatc tgataagtga
     2281 gctattcact ttaggtttag gatgaaaata ttctcttgga accatactta atatagaaat
     2341 atcaacttct gccattaaaa gtaatgccaa tgagcgtttt gtatttaata atcttttagc
     2401 aaacccgtat tccacgatta aataaatctc attagctata ctatcaaaaa caattttgcg
     2461 tattatatcc gtacttatgt tataaggtat attaccatat attttatagg attggttttt
     2521 aggaaattta aactgcaata tatccttgtt taaaacttgg aaattatcgt gatcttcctt
     2581 caggttatga ccatctgtgc cagttcgtaa tgtctggtca actttccgac tctgagaaac
     2641 ttctggaatc gctagagaat ttctggaatg ggattcagga gtggacagaa cgacacggat
     2701 atatagtgga tgtgtcaaaa cgcataccat tttgaacgat gacctctaat aattgttaat
     2761 catgttggtt acgtatttat taacttctcc tagtattagt aattatcatg gctgtcatgg
     2821 cgcattaacg gaataaaggg tgtgcttaaa tcgggccatt ttgcgtaata agaaaaagga
     2881 ttaattatga gcgaattgaa ttaataataa ggtaatagat ttacattaga aaatgaaagg
     2941 ggattttatg cgtgagaatg ttacagtcta tcccggcaat agttaccctt attatcaaga
     3001 taagaaagaa aaggattttt cgctacgctc aaatccttta aaaaaacaca aaagaccaca
     3061 ttttttaatg tggtctttta ttcttcaact aaagcaccca ttagttcaac aaacgaaaat
     3121 tggataaagt gggatatttt taaaatatat atatttatgt tacagtaata ttgactttta
     3181 aaaaaggatt gattctaatg aagaaagcag acaagtaagc ctcctaaatt cactttagat
     3241 aaaaatttag gaggcatatc aaatgaactt taataaaatt gatttagaca attggaagag
     3301 aaaagagata tttaatcatt atttgaacca acaaacgact tttagtataa ccacagaaat
     3361 tgatattagt gttttatacc gaaacataaa acaagaagga tataaatttt accctgcatt
     3421 tattttctta gtgacaaggg tgataaactc aaatacagct tttagaactg gttacaatag
     3481 cgacggagag ttaggttatt gggataagtt agagccactt tatacaattt ttgatggtgt
     3541 atctaaaaca ttctctggta tttggactcc tgtaaagaat gacttcaaag agttttatga
     3601 tttatacctt tctgatgtag agaaatataa tggttcgggg aaattgtttc ccaaaacacc
     3661 tatacctgaa aatgcttttt ctctttctat tattccatgg acttcattta ctgggtttaa
     3721 cttaaatatc aataataata gtaattacct tctacccatt attacagcag gaaaattcat
     3781 taataaaggt aattcaatat atttaccgct atctttacag gtacatcatt ctgtttgtga
     3841 tggttatcat gcaggattgt ttatgaactc tattcaggaa ttgtcagata ggcctaatga
     3901 ctggctttta taatatgaga taatgccgac tgtacttttt acagtcggtt ttctaacgat
     3961 acattaatag gtacgaaaaa gcaacttttt ttgcgcttaa aaccagtcat accaataact
     4021 taagggtaac tagcctcgcc ggaaagagcg aaaatgcctc acatttgtgc cacctaaaaa
     4081 ggagcgattt acatatgagt tatgcagttt gtagaatgca aaaagtgaaa tcagctggac
     4141 taaaaggcat gcaatttcat aatcaaagag agcgaaaaag tagaacgaat gatgatattg
     4201 accatgagcg aacacgtgaa aattatgatt tgaaaaatga taaaaatatt gattacaacg
     4261 aacgtgtcaa agaaattatt gaatcacaaa aaacaggtac aagaaaaacg aggaaagatg
     4321 ctgttcttgt aaatgagttg ctagtaacat ctgaccgaga tttttttgag caactggatc
     4381 gatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga
     4441 agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca
     4501 tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactta tcctaacagc
     4561 acaagagcgt cgaccttcta cacctccata tcacaaaaat tataacatta ttttgacata
     4621 aatactacat ttgtaatata ctacaaatgt agtcttatat aaggaggata atgggttttt
     4681 ttagcaaaat cactgataag cagaagcata ccataaataa aactaaaaaa ggggcaggag
     4741 caaccatgaa gcgcaaagtg gctatttata caagagtaag taccaaagaa caattgaaac
     4801 aaggctattc tattcaacaa caagcagact tactgattaa aaaagcaaag caaatatttc
     4861 cggatgatga atatgaaatt tatagtgatg aaggtatctc tggtaagaat atcgaagcaa
     4921 gacctggaat gaaaagatta ttagaagata tagaaaatcg acaaattaag gttgtgatga
     4981 gttggaaact taatcgtctt tcacgttcta atcgagatat acaaaatatt atttacgaat
     5041 ttagaagagg tggcgcctat tatatctcta tttcagaaaa cattgataca tcaacacaaa
     5101 atggcgaaat gatgataggt gcatttggat tagttgcaca gatagaacga gaaaccattg
     5161 ttagtaacgt aaagatggga atgaatgcga aagcgaaaca aggtgaagct attacaggta
     5221 gggtgctagg ttatgtttta aaacctaatc ctctaacagg taaaaatgaa cttgtcattg
     5281 acgaatttga agccaatata gtcagacaaa tttttgacct gtatttaaat caaaataaag
     5341 gattaaaagc aattgcgaat tatttaaata aacagggcta tcatacgata aataaaaagc
     5401 catttagcgt atatggagtg aaatatattc tgaataatcc cgtgtacaaa ggctatgttc
     5461 gatttaataa ttatcaaaat tgggcagtgg aacgacgaag tggcaaaaat gataaaagta
     5521 aagtgatatt agttcaaggt aagcacaaag cgattattga tgaagagatt ttcgataaaa
     5581 cacatgagaa gttagctgct aaaagtttta aacctggtcg accaattggt ggcgatttct
     5641 ggttgagagg gttaatcaaa tgtcctgagt gtggaaataa tatggtgtgt agacgaacat
     5701 attacaatac aaagaaatca aatgagagaa cgattaaacg ttattacatt tgttcattat
     5761 tcaatcgtgc aggtagtgca gcttgtcata gtaatgcgat taaggcagaa gtagtcgaac
     5821 gtgtggtata ttttcattta agacgcatcc tatctcaacc tcatgtggtc aaaacgatcg
     5881 caacacaggt tatcgatgct atggaaaaga aaaaagcaaa ccaaacgcca atgagtattg
     5941 atacaaattc attagagaat caaaagaaaa agttacaaaa aagaaaagaa cggttgattg
     6001 atttatattt agatgaagaa atagataaag aaacgatgca aaacaaacaa gataaactta
     6061 atgtgcaact ggaaaaaata gatgaacagt taaagcaggc tgaggttatg aataaagcat
     6121 ctcaagatat agccatacct aattataaga aaataaaagg tcaattatat gtgatgctat
     6181 accgatttac aggctacatg aaaaaagcaa atcccgaagc aaagaataaa ttaatgcata
     6241 tgttaatcga ttctatagaa cttacgaccg acaaacaagt taaactgatt aaatataaaa
     6301 tagatgaatc tcttctccca caatccatta aaaaggattg tgggaatttt tttatgccta
     6361 aatttaattt tgtgataaat gtcacaaaga aaaataggat tgaaaattta tcacttttac
     6421 cactttttta ataagaattc gatgtacgtc tcatcaatac gccatttgta ataagctttt
     6481 ttatgctttt tcttccaaat ttgatataaa attggtgcat attcttgaac ccaacggtag
     6541 accgttgaat gatgaacgtt tacaccacgt tcccttaata tttcagatat atcacgataa
     6601 ctcaatgcat atcttagata gtagccaacg gctacagtga taacatcctt gttaaattgt
     6661 ttatatctga aatagttcat acagaagact ccttttggtt ataattatac tataaattca
     6721 actttgcaac agaaccgtat attttatata gagcattctc actaaaaaaa ttacacatat
     6781 cgtgagcaat gaactgatta tacttaacat taaaaaagat gataacacct tctacacctc
     6841 catatcacaa aaattataac attattttga cataaatact acatttgtaa tatactacaa
     6901 atgtagtctt atataaggag gatattgatg aaaaagataa aaattgttcc acttatttta
     6961 atagttgtag ttgtcgggtt tggtatatat ttttatgctt caaaagataa agaaattaat
     7021 aatactattg atgcaattga agataaaaat ttcaaacaag tttataaaga tagcagttat
     7081 atttctaaaa gcgataatgg tgaagtagaa atgactgaac gtccgataaa aatatataat
     7141 agtttaggcg ttaaagatat aaacattcag gatcgtaaaa taaaaaaagt atctaaaaat
     7201 aaaaaacgag tagatgctca atataaaatt aaaacaaact acggtaacat tgatcgcaac
     7261 gttcaattta attttgttaa agaagatggt atgtggaagt tagattggga tcatagcgtc
     7321 attattccag gaatgcagaa agaccaaagc atacatattg aaaatttaaa atcagaacgt
     7381 ggtaaaattt tagaccgaaa caatgtggaa ttggccaata caggaacagc atatgagata
     7441 ggcatcgttc caaagaatgt atctaaaaaa gattataaag caatcgctaa agaactaagt
     7501 atttctgaag actatatcaa acaacaaatg gatcaaaatt gggtacaaga tgataccttc
     7561 gttccactta aaaccgttaa aaaaatggat gaatatttaa gagatttcgc aaaaaaattt
     7621 catcttacaa ctaatgaaac agaaagtcgt aactatcctc tagaaaaagc gacttcacat
     7681 ctattaggtt atgttggtcc cattaactct gaagaattaa aacaaaaaga atataaaggc
     7741 tataaagatg atgcagttat tggtaaaaag ggactcgaaa aactttacga taaaaagctc
     7801 caacatgaag atggctatcg tgtcacaatc gttgacgata atagcaatac aatcgcacat
     7861 acattaatag agaaaaagaa aaaagatggc aaagatattc aactaactat tgatgctaaa
     7921 gttcaaaaga gtatttataa caacatgaaa aatgattatg gctcaggtac tgctatccac
     7981 cctcaaacag gtgaattatt agcacttgta agcacacctt catatgacgt ctatccattt
     8041 atgtatggca tgagtaacga agaatataat aaattaaccg aagataaaaa agaacctctg
     8101 ctcaacaagt tccagattac aacttcacca ggttcaactc aaaaaatatt aacagcaatg
     8161 attgggttaa ataacaaaac attagacgat aaaacaagtt ataaaatcga tggtaaaggt
     8221 tggcaaaaag ataaatcttg gggtggttac aacgttacaa gatatgaagt ggtaaatggt
     8281 aatatcgact taaaacaagc aatagaatca tcagataaca ttttctttgc tagagtagca
     8341 ctcgaattag gcagtaagaa atttgaaaaa ggcatgaaaa aactaggtgt tggtgaagat
     8401 ataccaagtg attatccatt ttataatgct caaatttcaa acaaaaattt agataatgaa
     8461 atattattag ctgattcagg ttacggacaa ggtgaaatac tgattaaccc agtacagatc
     8521 ctttcaatct atagcgcatt agaaaataat ggcaatatta acgcacctca cttattaaaa
     8581 gacacgaaaa acaaagtttg gaagaaaaat attatttcca aagaaaatat caatctatta
     8641 actgatggta tgcaacaagt cgtaaataaa acacataaag aagatattta tagatcttat
     8701 gcaaacttaa ttggcaaatc cggtactgca gaactcaaaa tgaaacaagg agaaactggc
     8761 agacaaattg ggtggtttat atcatatgat aaagataatc caaacatgat gatggctatt
     8821 aatgttaaag atgtacaaga taaaggaatg gctagctaca atgccaaaat ctcaggtaaa
     8881 gtgtatgatg agctatatga gaacggtaat aaaaaatacg atatagatga ataataagcg
     8941 gccgctcgag catgcatcta gagggcccaa ttcgccctat agtgagtcgt attacaattc
     9001 actggccgtc gttttacaaa cgcctggggt aatgactctc tagcttgagg catcaaataa
     9061 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     9121 ctctcctgag taggacaaat ccgccgctct agctagaatt aattcttgaa gacgaaaggg
     9181 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     9241 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     9301 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     9361 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     9421 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     9481 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     9541 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     9601 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     9661 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     9721 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     9781 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     9841 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     9901 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     9961 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
    10021 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
    10081 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
    10141 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
    10201 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
    10261 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    10321 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    10381 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    10441 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    10501 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
    10561 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    10621 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    10681 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    10741 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    10801 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    10861 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    10921 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    10981 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    11041 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    11101 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    11161 ggaagcggaa ga
//
