pSCCcat vector (V003416)

LOCUS       V003416                 8695 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003416
VERSION     V003416
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8695)
  AUTHORS   Wu Z.
  TITLE     Single large serine recombinase mediates the horizontal transfer of
            SCCmec in staphylococci
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8695)
  AUTHORS   Wu Z.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8695)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8695)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-MAR-2017) College of Animal Science "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(4..144)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             1065..1232
                     /codon_start=1
                     /product="Cop-6"
                     /label="Cop-6"
                     /protein_id="ASN64779.1"
                     /translation="MVVDRKEEKKVAVTLRLTTEENEILNRIKEKYNISKSDATGILIK
                     KYAKEEYGAF"
     CDS             1313..1912
                     /codon_start=1
                     /product="RepF"
                     /label="RepF"
                     /note="TS replicon from pE194"
                     /protein_id="ASN64780.1"
                     /translation="MSENVIKETENKKNSRGRNWTFVLYPESAKAEWLEYLKELHIQFV
                     VSPLHDRDTDTEDRMKKEHYHILVMYEGNKSYEQIKIITEELNATIPQIAGSVKGLVRY
                     MLHMDDPNKFKYQKEDMIVYGGVDVDELLKKTTTDRYKLIKEMIEFIDEQGIVEFKSLM
                     DYAMKFKFDDWFPLLCDNSAYVIQEYIKSNRYKSDR"
     CDS             3263..3910
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     regulatory      4575..4670
                     /label="PmecA"
                     /note="PmecA"
                     /regulatory_class="promoter"
     CDS             4671..6431
                     /codon_start=1
                     /product="N-terminal-tagged CcrC2"
                     /label="N-terminal-tagged CcrC2"
                     /protein_id="ASN64782.1"
                     /translation="MGFFSKITDKQKHTINKTKKGAGATMKRKVAIYTRVSTKEQLKQG
                     YSIQQQADLLIKKAKQIFPDDEYEIYSDEGISGKNIEARPGMKRLLEDIENRQIKVVMS
                     WKLNRLSRSNRDIQNIIYEFRRGGAYYISISENIDTSTQNGEMMIGAFGLVAQIERETI
                     VSNVKMGMNAKAKQGEAITGRVLGYVLKPNPLTGKNELVIDEFEANIVRQIFDLYLNQN
                     KGLKAIANYLNKQGYHTINKKPFSVYGVKYILNNPVYKGYVRFNNYQNWAVERRSGKND
                     KSKVILVQGKHKAIIDEEIFDKTHEKLAAKSFKPGRPIGGDFWLRGLIKCPECGNNMVC
                     RRTYYNTKKSNERTIKRYYICSLFNRAGSAACHSNAIKAEVVERVVYFHLRRILSQPHV
                     VKTIATQVIDAMEKKKANQTPMSIDTNSLENQKKKLQKRKERLIDLYLDEEIDKETMQN
                     KQDKLNVQLEKIDEQLKQAEVMNKASQDIAIPNYKKIKGQLYVMLYRFTGYMKKANPEA
                     KNKLMHMLIDSIELTTDKQVKLIKYKIDESLLPQSIKKDCGNFFMPKFNFVINVTKKNR
                     IENLSLLPLF"
     repeat_region   4685..4723
                     /label="attSCC"
                     /note="attSCC"
     promoter        complement(6499..6517)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6524..6540)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      6577..6663
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        6789..6893
                     /label="AmpR promoter"
     CDS             6894..7751
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7925..8513
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"