LOCUS       V003416                 8695 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003416
VERSION     V003416
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8695)
  AUTHORS   Wu Z.
  TITLE     Single large serine recombinase mediates the horizontal transfer of
            SCCmec in staphylococci
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8695)
  AUTHORS   Wu Z.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8695)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8695)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-MAR-2017) College of Animal Science "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(4..144)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             1065..1232
                     /codon_start=1
                     /product="Cop-6"
                     /label="Cop-6"
                     /protein_id="ASN64779.1"
                     /translation="MVVDRKEEKKVAVTLRLTTEENEILNRIKEKYNISKSDATGILIK
                     KYAKEEYGAF"
     CDS             1313..1912
                     /codon_start=1
                     /product="RepF"
                     /label="RepF"
                     /note="TS replicon from pE194"
                     /protein_id="ASN64780.1"
                     /translation="MSENVIKETENKKNSRGRNWTFVLYPESAKAEWLEYLKELHIQFV
                     VSPLHDRDTDTEDRMKKEHYHILVMYEGNKSYEQIKIITEELNATIPQIAGSVKGLVRY
                     MLHMDDPNKFKYQKEDMIVYGGVDVDELLKKTTTDRYKLIKEMIEFIDEQGIVEFKSLM
                     DYAMKFKFDDWFPLLCDNSAYVIQEYIKSNRYKSDR"
     CDS             3263..3910
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     regulatory      4575..4670
                     /label="PmecA"
                     /note="PmecA"
                     /regulatory_class="promoter"
     CDS             4671..6431
                     /codon_start=1
                     /product="N-terminal-tagged CcrC2"
                     /label="N-terminal-tagged CcrC2"
                     /protein_id="ASN64782.1"
                     /translation="MGFFSKITDKQKHTINKTKKGAGATMKRKVAIYTRVSTKEQLKQG
                     YSIQQQADLLIKKAKQIFPDDEYEIYSDEGISGKNIEARPGMKRLLEDIENRQIKVVMS
                     WKLNRLSRSNRDIQNIIYEFRRGGAYYISISENIDTSTQNGEMMIGAFGLVAQIERETI
                     VSNVKMGMNAKAKQGEAITGRVLGYVLKPNPLTGKNELVIDEFEANIVRQIFDLYLNQN
                     KGLKAIANYLNKQGYHTINKKPFSVYGVKYILNNPVYKGYVRFNNYQNWAVERRSGKND
                     KSKVILVQGKHKAIIDEEIFDKTHEKLAAKSFKPGRPIGGDFWLRGLIKCPECGNNMVC
                     RRTYYNTKKSNERTIKRYYICSLFNRAGSAACHSNAIKAEVVERVVYFHLRRILSQPHV
                     VKTIATQVIDAMEKKKANQTPMSIDTNSLENQKKKLQKRKERLIDLYLDEEIDKETMQN
                     KQDKLNVQLEKIDEQLKQAEVMNKASQDIAIPNYKKIKGQLYVMLYRFTGYMKKANPEA
                     KNKLMHMLIDSIELTTDKQVKLIKYKIDESLLPQSIKKDCGNFFMPKFNFVINVTKKNR
                     IENLSLLPLF"
     repeat_region   4685..4723
                     /label="attSCC"
                     /note="attSCC"
     promoter        complement(6499..6517)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6524..6540)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      6577..6663
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        6789..6893
                     /label="AmpR promoter"
     CDS             6894..7751
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7925..8513
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
       61 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
      121 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
      181 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
      241 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcaggaacg gattgttgat
      301 gattacgaaa atattaagag cacagactat tacacagaaa atcaagaatt aaaaaaacgt
      361 agagagagtt tgaaagaagt agtgaataca tggaaagagg ggtatcacga aaaaagtaaa
      421 gaggttaata aattaaagcg agagaatgat agtttgaatg agcagttgaa tgtatcagag
      481 aaatttcaag atagtacagt gactttatat cgtgctgcga gggcgaattt ccctgggttt
      541 gagaaagggt ttaataggct taaagagaaa ttctttaatg attccaaatt cgagcgtgtg
      601 ggacagttta tggatgttgt acaggataat gtccagaagg tcgatagaaa gcgtgagaaa
      661 cagcgtacag acgatttaga gatgtagagg tacttttatg ccgagaaaac tttttgcgtg
      721 tgacagtcct taaaatatac ttagagcgta agcgaaagta gtagcgacag ctattaactt
      781 tcggttgcaa agctctagga tttttaatgg acgcagcgca tcacacgcaa aaaggaaatt
      841 ggaataaatg cgaaatttga gatgttaatt aaagaccttt ttgaggtctt tttttcttag
      901 atttttgggg ttatttaggg gagaaaacat aggggggtac tacgacctcc cccctaggtg
      961 tccattgtcc attgtccaaa caaataaata aatattgggt ttttaatgtt aaaaggttgt
     1021 tttttatgtt aaagtgaaaa aaacagatgt tgggaggtac agtgatggtt gtagatagaa
     1081 aagaagagaa aaaagttgct gttactttaa gacttacaac agaagaaaat gagatattaa
     1141 atagaatcaa agaaaaatat aatattagca aatcagatgc aaccggtatt ctaataaaaa
     1201 aatatgcaaa ggaggaatac ggtgcatttt aaacaaaaaa agatagacag cactggcatg
     1261 ctgcctatct atgactaaat tttgttaaat gtattagcac cgttattata tcatgagcga
     1321 aaatgtaata aaagaaactg aaaacaagaa aaattcaaga ggacgtaatt ggacatttgt
     1381 tttatatcca gaatcagcaa aagccgagtg gttagagtat ttaaaagagt tacacattca
     1441 atttgtagtg tctccattac atgataggga tactgataca gaagatagga tgaaaaaaga
     1501 gcattatcat attctagtga tgtatgaggg taataaatct tatgaacaga taaaaataat
     1561 tacagaagaa ttgaatgcga ctattccgca gattgcagga agtgtgaaag gtcttgtgag
     1621 atatatgctt cacatggacg atcctaataa atttaaatat caaaaagaag atatgatagt
     1681 ttatggcggt gtagatgttg atgaattatt aaagaaaaca acaacagata gatataaatt
     1741 aattaaagaa atgattgagt ttattgatga acaaggaatc gtagaattta agagtttaat
     1801 ggattatgca atgaagttta aatttgatga ttggttcccg cttttatgtg ataactcggc
     1861 gtatgttatt caagaatata taaaatcaaa tcggtataaa tctgaccgat agattttgaa
     1921 tttaagagtg tcacaagaca ctcttttttc gcaccagcga aaactggttt aagccgactg
     1981 cgcaaaagac ataatcgatt cacaaaaaat aggcacacga aaaacaagtt aagggatgca
     2041 gtttatgcat cccttaactt acttattaaa taatttatag ctattgaaaa gagataagaa
     2101 ttgttcaaag ctaatattgt ttaaatcgtc aattcctgca tgttttaagg aattgttaaa
     2161 ttgatttttt gtaaatattt tcttgtattc tttgttaacc catttcataa cgaaataatt
     2221 atacttttgt ttatctttgt gtgatattct tgattttttt ctacttaatc tgataagtga
     2281 gctattcact ttaggtttag gatgaaaata ttctcttgga accatactta atatagaaat
     2341 atcaacttct gccattaaaa gtaatgccaa tgagcgtttt gtatttaata atcttttagc
     2401 aaacccgtat tccacgatta aataaatctc attagctata ctatcaaaaa caattttgcg
     2461 tattatatcc gtacttatgt tataaggtat attaccatat attttatagg attggttttt
     2521 aggaaattta aactgcaata tatccttgtt taaaacttgg aaattatcgt gatcttcctt
     2581 caggttatga ccatctgtgc cagttcgtaa tgtctggtca actttccgac tctgagaaac
     2641 ttctggaatc gctagagaat ttctggaatg ggattcagga gtggacagaa cgacacggat
     2701 atatagtgga tgtgtcaaaa cgcataccat tttgaacgat gacctctaat aattgttaat
     2761 catgttggtt acgtatttat taacttctcc tagtattagt aattatcatg gctgtcatgg
     2821 cgcattaacg gaataaaggg tgtgcttaaa tcgggccatt ttgcgtaata agaaaaagga
     2881 ttaattatga gcgaattgaa ttaataataa ggtaatagat ttacattaga aaatgaaagg
     2941 ggattttatg cgtgagaatg ttacagtcta tcccggcaat agttaccctt attatcaaga
     3001 taagaaagaa aaggattttt cgctacgctc aaatccttta aaaaaacaca aaagaccaca
     3061 ttttttaatg tggtctttta ttcttcaact aaagcaccca ttagttcaac aaacgaaaat
     3121 tggataaagt gggatatttt taaaatatat atatttatgt tacagtaata ttgactttta
     3181 aaaaaggatt gattctaatg aagaaagcag acaagtaagc ctcctaaatt cactttagat
     3241 aaaaatttag gaggcatatc aaatgaactt taataaaatt gatttagaca attggaagag
     3301 aaaagagata tttaatcatt atttgaacca acaaacgact tttagtataa ccacagaaat
     3361 tgatattagt gttttatacc gaaacataaa acaagaagga tataaatttt accctgcatt
     3421 tattttctta gtgacaaggg tgataaactc aaatacagct tttagaactg gttacaatag
     3481 cgacggagag ttaggttatt gggataagtt agagccactt tatacaattt ttgatggtgt
     3541 atctaaaaca ttctctggta tttggactcc tgtaaagaat gacttcaaag agttttatga
     3601 tttatacctt tctgatgtag agaaatataa tggttcgggg aaattgtttc ccaaaacacc
     3661 tatacctgaa aatgcttttt ctctttctat tattccatgg acttcattta ctgggtttaa
     3721 cttaaatatc aataataata gtaattacct tctacccatt attacagcag gaaaattcat
     3781 taataaaggt aattcaatat atttaccgct atctttacag gtacatcatt ctgtttgtga
     3841 tggttatcat gcaggattgt ttatgaactc tattcaggaa ttgtcagata ggcctaatga
     3901 ctggctttta taatatgaga taatgccgac tgtacttttt acagtcggtt ttctaacgat
     3961 acattaatag gtacgaaaaa gcaacttttt ttgcgcttaa aaccagtcat accaataact
     4021 taagggtaac tagcctcgcc ggaaagagcg aaaatgcctc acatttgtgc cacctaaaaa
     4081 ggagcgattt acatatgagt tatgcagttt gtagaatgca aaaagtgaaa tcagctggac
     4141 taaaaggcat gcaatttcat aatcaaagag agcgaaaaag tagaacgaat gatgatattg
     4201 accatgagcg aacacgtgaa aattatgatt tgaaaaatga taaaaatatt gattacaacg
     4261 aacgtgtcaa agaaattatt gaatcacaaa aaacaggtac aagaaaaacg aggaaagatg
     4321 ctgttcttgt aaatgagttg ctagtaacat ctgaccgaga tttttttgag caactggatc
     4381 gatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga
     4441 agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca
     4501 tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactta tcctaacagc
     4561 acaagagcgt cgaccttcta cacctccata tcacaaaaat tataacatta ttttgacata
     4621 aatactacat ttgtaatata ctacaaatgt agtcttatat aaggaggata atgggttttt
     4681 ttagcaaaat cactgataag cagaagcata ccataaataa aactaaaaaa ggggcaggag
     4741 caaccatgaa gcgcaaagtg gctatttata caagagtaag taccaaagaa caattgaaac
     4801 aaggctattc tattcaacaa caagcagact tactgattaa aaaagcaaag caaatatttc
     4861 cggatgatga atatgaaatt tatagtgatg aaggtatctc tggtaagaat atcgaagcaa
     4921 gacctggaat gaaaagatta ttagaagata tagaaaatcg acaaattaag gttgtgatga
     4981 gttggaaact taatcgtctt tcacgttcta atcgagatat acaaaatatt atttacgaat
     5041 ttagaagagg tggcgcctat tatatctcta tttcagaaaa cattgataca tcaacacaaa
     5101 atggcgaaat gatgataggt gcatttggat tagttgcaca gatagaacga gaaaccattg
     5161 ttagtaacgt aaagatggga atgaatgcga aagcgaaaca aggtgaagct attacaggta
     5221 gggtgctagg ttatgtttta aaacctaatc ctctaacagg taaaaatgaa cttgtcattg
     5281 acgaatttga agccaatata gtcagacaaa tttttgacct gtatttaaat caaaataaag
     5341 gattaaaagc aattgcgaat tatttaaata aacagggcta tcatacgata aataaaaagc
     5401 catttagcgt atatggagtg aaatatattc tgaataatcc cgtgtacaaa ggctatgttc
     5461 gatttaataa ttatcaaaat tgggcagtgg aacgacgaag tggcaaaaat gataaaagta
     5521 aagtgatatt agttcaaggt aagcacaaag cgattattga tgaagagatt ttcgataaaa
     5581 cacatgagaa gttagctgct aaaagtttta aacctggtcg accaattggt ggcgatttct
     5641 ggttgagagg gttaatcaaa tgtcctgagt gtggaaataa tatggtgtgt agacgaacat
     5701 attacaatac aaagaaatca aatgagagaa cgattaaacg ttattacatt tgttcattat
     5761 tcaatcgtgc aggtagtgca gcttgtcata gtaatgcgat taaggcagaa gtagtcgaac
     5821 gtgtggtata ttttcattta agacgcatcc tatctcaacc tcatgtggtc aaaacgatcg
     5881 caacacaggt tatcgatgct atggaaaaga aaaaagcaaa ccaaacgcca atgagtattg
     5941 atacaaattc attagagaat caaaagaaaa agttacaaaa aagaaaagaa cggttgattg
     6001 atttatattt agatgaagaa atagataaag aaacgatgca aaacaaacaa gataaactta
     6061 atgtgcaact ggaaaaaata gatgaacagt taaagcaggc tgaggttatg aataaagcat
     6121 ctcaagatat agccatacct aattataaga aaataaaagg tcaattatat gtgatgctat
     6181 accgatttac aggctacatg aaaaaagcaa atcccgaagc aaagaataaa ttaatgcata
     6241 tgttaatcga ttctatagaa cttacgaccg acaaacaagt taaactgatt aaatataaaa
     6301 tagatgaatc tcttctccca caatccatta aaaaggattg tgggaatttt tttatgccta
     6361 aatttaattt tgtgataaat gtcacaaaga aaaataggat tgaaaattta tcacttttac
     6421 cactttttta ataagaattc tgcagatatc catcacactg gcggccgctc gagcatgcat
     6481 ctagagggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
     6541 aaacgcctgg ggtaatgact ctctagcttg aggcatcaaa taaaacgaaa ggctcagtcg
     6601 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     6661 aatccgccgc tctagctaga attaattctt gaagacgaaa gggcctcgtg atacgcctat
     6721 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     6781 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6841 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6901 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6961 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7021 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7081 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     7141 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7201 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7261 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7321 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7381 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7441 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7501 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7561 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7621 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7681 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7741 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7801 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7861 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7921 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7981 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8041 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     8101 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8161 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8221 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8281 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8341 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8401 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8461 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8521 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8581 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8641 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaaga
//
