pSCBe vector (V003418)

LOCUS       40924_38958        6633 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSCBe, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Jin H, Wang Y, Idoine A, Bhaya D.
  TITLE     Construction of a Shuttle Vector Using an Endogenous Plasmid From 
            the Cyanobacterium Synechocystis sp. PCC6803
  JOURNAL   Front Microbiol 9, 1662 (2018)
  PUBMED    30087668
REFERENCE   2  (bases 1 to 6633)
  AUTHORS   Haojie J, Devaki B.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAR-2018) Plant Biology Department, Carnegie 
            Institution for Science, 260 Panama Street, Stanford, CA 94305, US
REFERENCE   3  (bases 1 to 6633)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6633)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol 9, 1662 (2018)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-MAR-2018) Plant Biology Department, Carnegie Institution for 
            Science, 260 Panama Street, Stanford, CA 94305, US"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            258..3173
                     /gene="ORFb"
                     /label=ORFb
     CDS             3798..3986
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    4091..4231
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4417..5005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5288..6076)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        6298..6327
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    6335..6351
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6369..6380
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             6390..6413
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             6477..6494
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      6512..6583
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      6599..6626
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"