LOCUS       40924_38958        6633 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSCBe, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Jin H, Wang Y, Idoine A, Bhaya D.
  TITLE     Construction of a Shuttle Vector Using an Endogenous Plasmid From 
            the Cyanobacterium Synechocystis sp. PCC6803
  JOURNAL   Front Microbiol 9, 1662 (2018)
  PUBMED    30087668
REFERENCE   2  (bases 1 to 6633)
  AUTHORS   Haojie J, Devaki B.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAR-2018) Plant Biology Department, Carnegie 
            Institution for Science, 260 Panama Street, Stanford, CA 94305, US
REFERENCE   3  (bases 1 to 6633)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6633)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol 9, 1662 (2018)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-MAR-2018) Plant Biology Department, Carnegie Institution for 
            Science, 260 Panama Street, Stanford, CA 94305, US"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            258..3173
                     /gene="ORFb"
                     /label=ORFb
     CDS             3798..3986
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    4091..4231
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4417..5005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5288..6076)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        6298..6327
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    6335..6351
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6369..6380
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             6390..6413
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             6477..6494
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      6512..6583
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      6599..6626
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 aaacagcgtg acagcgaccg atctaatccg ttacaattct ggcaatagtc caagatttcc
       61 aacaaaaaaa agcttttcag gagggaatta agattgctgc agtaaaaaac gtaagaagtt
      121 tagttgacgc taaaaaactt acctacagac aataacccgg cccaaaaagc caacaaaata
      181 cttcaaaaat attgtctcta ctgtagctct aaaaattccc aaaagaaaag cggtcaactc
      241 ttgaacccga gaccgctatg aacaattata gtctttccca ccaaaattct caagccttag
      301 cccatcagca tttcaacgga tggcaagggt caaaaaatga cccaaaaata cggtcagaat
      361 ttgtagaaga ttcggcgatc gcccctgacg tattccgggc caacgtggag attgtgccgg
      421 atcttgagat agaccccatc accaaggaag tcttggcaac gccgatcgcc gatgccctgg
      481 ggcgaaaatt tacccgcttt ggacatcaag ccaaggaaga caggcaggcc gccattttcc
      541 ggtctgagaa tgggaacgta tggcaggtaa aaatctttgg tgaagacaag acgggcaagc
      601 ggtcagggca gtatctagca ccgacaggca taggggacgt gccctatctg ccaacaattc
      661 cccgccggat cattttggcg atcgccgaaa agcatggggt caaaccaccg gaagatggtc
      721 aagatttttg gccctggttt gtggatcatc ctgagattcc cttgattgtg acggaagggg
      781 ggaagaaagc tttagccgcc atcagccaag gttacgtggc cctgagtctc tatggctgtt
      841 tatgtggcaa cgatggtcta accatcaaac cgtcattatt gccctatgtg caggggcgag
      901 aagtagcgat cgcctatgac caggatgcaa agggtagcaa gggacgaaag gcagtcttta
      961 agggcacaaa acggctggct cgtaacctga cctatcacgc taaggcaacg gtcaagatct
     1021 gccaatggaa cggggaagac ggcaagggca ttgatgactt gatcgccaat aaccctgcta
     1081 aatttcaccg ggcgatcgct cggggcatca gcctagaaca gttccaaaac aagccgtatg
     1141 ctgacctatc ccaatgggtg aatcaaaccc ggcacgaaag atacctaagc aacattaatg
     1201 cccctaccac cgccaaaata atcgggatta aatcagccaa gggcacggga aagacggaag
     1261 cgatctcaaa aatggtggag caacggcgag aagattttcg ccggacttat ccactcactc
     1321 accgaattca actaggcatt gccctatccg gccggtttgg tgtggactac atcagagatt
     1381 tgtccgagag cgacactaat ggggccttag gaatcggatt gtgcattgat agcatcctga
     1441 aacttgaccc cgaaacattc cggggccatg acatcatcct tgatgaatgc gagcaattga
     1501 tatgggaatt attagatagt tcccggggaa acctgggcaa acataaacca gccatcttgg
     1561 cccgcttcca agaaatattg atcgccgccg ctgattcggg gggcaagatt attttgtctg
     1621 atgctgattt gtccccggtg actatcaagt atgtccaaga cttaatcggt ggtaaggcag
     1681 aatgctatat tgtcgttaac acgtttaagc cagtgcagga taagcgtaat ttgattgttt
     1741 ataattcccc ggaagatttg cttacttccg ctattaatac tgttgctaat ggtaaaaaaa
     1801 tacagatttg caccggggca caaaaagcac agtctatcta ttcaacaact aacctagggg
     1861 acttattcaa aatgttattc cctgataagt cagtaggggt cttagatgcc catacagtaa
     1921 gcgataagac taaccctgcc tatggctgta tggataactt agatagatat ttagaagaca
     1981 aggatattgt catcacgtcc ccggtaatag agacgggaat cagcattgat aagccctatt
     2041 ttgatgaagt ctatggattc ggacagggaa cacaaacagt agagcaattc tgccaaggat
     2101 tagaaaggta tcgtcccgat gtggatcgcc atgtttggat tcgcaatcgt gcccctaata
     2161 attgttttat tgctggcggg gaaactaacc ctcgctatat ccttaatgtg gagaataaga
     2221 aagccaaggc tactatcggg gcattgaata cagccgacaa tttaaccctg tttgatgatg
     2281 agaagccaga gcatcttaaa acctgggcta ttatggcggc tctgcacaat caagggaaga
     2341 aaagcttaaa atcaactact cttgaaaaat taggggaaga agggtacaca cagaaagaac
     2401 ctgacccaga tatggtcacc ggggcggaag acgtagctga tcgcattaaa gctatccgtg
     2461 accataacta tggaaaggaa accacggcga tcgccgagag ccttaatccc gatgacttca
     2521 cctatgaaag tttgaagcgg aagcaagtta agacggaagc ggaacgatat caagagcgga
     2581 agggagatct ttgccgtgcc ctggctaccg atgacataac cccagaagat gtggagaaat
     2641 gtgatcaagg atggctggcc caattgaccc tgcattatta cttcaccacc ggtgcagaat
     2701 ttttacaggc aagggacagc caaaggctcc aaaatttggc cggggaagat ggaaaggttt
     2761 gtgccaagga tgctagccgg aagactctca ccgtccccgt tcacttcctt aaagtattgg
     2821 gctttgaaag attccttacc gggatagggg aattttccaa ggattcccta gccaattggt
     2881 ttgagaagac tatcctgccc aacaaggcag aaattaagca ggccttgggc atcacgatca
     2941 accctgacaa aggggcgatc gccttcgtgc aatctgtctt gaaaaaaatg ggtttacatc
     3001 ttgagtatct gggccggttt ggggaacggg gcaatactaa gcgacattat ggccccgcca
     3061 atattgaccc cgatgggcga tcggctattt tttcccggtg gtttgagcgg gattctattt
     3121 tgtatcactc tgataccgta tccactgaat ccttattaat taatcaagcc taggaactgg
     3181 ataccaaaaa cagggagtta tgatgggaat ataatcccgt taacaggcta aacccatgca
     3241 ggcaaaccaa cccttggcag aacatatcca tcgcgtccgc catctccagc agccgcacgc
     3301 ggcgcatctc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt
     3361 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac
     3421 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa
     3481 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca
     3541 ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg
     3601 tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata
     3661 ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc
     3721 gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc
     3781 cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta
     3841 tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg
     3901 cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc
     3961 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     4021 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     4081 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     4141 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     4201 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     4261 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     4321 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     4381 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4441 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4501 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4561 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4621 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4681 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4741 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4801 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     4861 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4921 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4981 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     5041 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     5101 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     5161 aacttggtct gacagacttg acctgatagt ttggctgtga gcaattatgt gcttagtgca
     5221 tctaacgctt gagttaagcc gcgccgcgaa gcggcgtcgg cttgaacgaa tttctagcta
     5281 gacattattt gccgactacc ttggtgatct cgcctttcac gtagtggaca aattcttcca
     5341 actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt
     5401 caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat
     5461 ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta
     5521 catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta
     5581 gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta
     5641 ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg
     5701 tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt
     5761 cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta
     5821 cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca
     5881 aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac
     5941 tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg
     6001 gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga
     6061 tcaccgcttc ccccatgatg tttaactttg ttttagggcg actgccctgc tgcgtaacat
     6121 cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg
     6181 cccgaggcat agactgtacc ccaaaaaaac atgtcataac aagaagccat gaaaaccgcc
     6241 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgacgcgttg
     6301 acaattaatc atccggctcg tataatgtgt ggaattgtga gcggataaca atttcacaca
     6361 ttctagagaa agaggagaaa tactagatgg actacaaaga cgatgacgac aagcttgcgg
     6421 ccgccgcggc atcgatagat ctgagctcgg taccagtcga ctcgagagga tcccggcacc
     6481 atcaccatca ccattaacta gtgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     6541 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     6601 ctcaccttcg ggtgggcctt tctgcgttta tat
//
