pSC-R1LambdaR2 vector (V003445)

LOCUS       V003445                 8092 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003445
VERSION     V003445
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Choi EW, Seen DS, Song YB, Son HS, Jung NC, Huh WK, Hahn JS, Kim K,
            Jeong JY, Lee TG.
  TITLE     AdHTS: a high-throughput system for generating recombinant
            adenoviruses
  JOURNAL   J. Biotechnol. 162 (2-3), 246-252 (2012)
   PUBMED   23063969
REFERENCE   2  (bases 1 to 8092)
  AUTHORS   Choi E-W., Seen D-S., Son H-S., Song YB, Huh W-K., Hahn J-S., Kim K,
            Jeong J-Y., Lee TG.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2011) R
REFERENCE   3  (bases 1 to 8092)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8092)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Biotechnol. 162 (2-3), 246-252 (2012)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-JUN-2011) R"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   4..106
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     194..344
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        402..705
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        706..909
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    949..1073
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1464..1588)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    1747..1868
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             1962..2381
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     CDS             complement(2447..3781)
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     repeat_region   5055..5157
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     rep_origin      complement(5417..6005)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             6837..7628
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(7679..8058)
                     /direction=LEFT
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"