LOCUS       V003445                 8092 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003445
VERSION     V003445
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Choi EW, Seen DS, Song YB, Son HS, Jung NC, Huh WK, Hahn JS, Kim K,
            Jeong JY, Lee TG.
  TITLE     AdHTS: a high-throughput system for generating recombinant
            adenoviruses
  JOURNAL   J. Biotechnol. 162 (2-3), 246-252 (2012)
   PUBMED   23063969
REFERENCE   2  (bases 1 to 8092)
  AUTHORS   Choi E-W., Seen D-S., Son H-S., Song YB, Huh W-K., Hahn J-S., Kim K,
            Jeong J-Y., Lee TG.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2011) R
REFERENCE   3  (bases 1 to 8092)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8092)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Biotechnol. 162 (2-3), 246-252 (2012)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-JUN-2011) R"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   4..106
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     194..344
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        402..705
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        706..909
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    949..1073
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1464..1588)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    1747..1868
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             1962..2381
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     CDS             complement(2447..3781)
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     repeat_region   5055..5157
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     rep_origin      complement(5417..6005)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             6837..7628
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(7679..8058)
                     /direction=LEFT
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 taacatcatc aataatatac cttattttgg attgaagcca atatgataat gagggggtgg
       61 agtttgtgac gtggcgcggg gcgtgggaac ggggcgggtg acgtagtagt gtggcggaag
      121 tgtgatgttg caagtgtggc ggaacacatg taagcgacgg atgtggcaaa agtgacgttt
      181 ttggtgtgcg ccggtgtaca caggaagtga caattttcgc gcggttttag gcggatgttg
      241 tagtaaattt gggcgtaacc gagtaagatt tggccatttt cgcgggaaaa ctgaataaga
      301 ggaagtgaaa tctgaataat tttgtgttac tcatagcgcg taatactgta atagtaatca
      361 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      421 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      481 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      541 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      601 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      661 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      721 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      781 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      841 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      901 agcagagctg gtttagtgaa ccgtcagatc cgctagagat ctggtaccac aagtttgtac
      961 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
     1021 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccg
     1081 catttaaata tcgattatca gctattgcca gcgccagata taagcgattt aagctaagaa
     1141 aacgcattaa gatgcaaaac gataaagtgc gatcagtaat tcaaaacctt acagaagagc
     1201 aatctatggt tttgtgcgca gcccttaatg aaggcaggaa gtatgtggtt acatcaaaac
     1261 aattcccata cattagtgag ttgattgagc ttggtgtgtt gaacaaaact ttttcccgat
     1321 ggaatggaaa gcatatatta ttccctattg aggatattta ctggactgaa ttagttgcca
     1381 gctatgatcc atataatatt gagataaagc caaggccaat atctaagtaa ctagataaga
     1441 ggaatcgata tttaaatgtc gaccatagtg actggatatg ttgtgtttta cagtattatg
     1501 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
     1561 ctcgttcagc tttcttgtac aaagtggtct cgagcctaag cttctagata agatatccga
     1621 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt
     1681 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     1741 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1801 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1861 gtatcttaac gcggatctgg gcgtggttaa gggtgggaaa gaatatataa ggtgggggtc
     1921 ttatgtagtt ttgtatctgt tttgcagcag ccgccgccgc catgagcacc aactcgtttg
     1981 atggaagcat tgtgagctca tatttgacaa cgcgcatgcc cccatgggcc ggggtgcgtc
     2041 agaatgtgat gggctccagc attgatggtc gccccgtcct gcccgcaaac tctactacct
     2101 tgacctacga gaccgtgtct ggaacgccgt tggagactgc agcctccgcc gccgcttcag
     2161 ccgctgcagc caccgcccgc gggattgtga ctgactttgc tttcctgagc ccgcttgcaa
     2221 gcagtgcagc ttcccgttca tccgcccgcg atgacaagtt gacggctctt ttggcacaat
     2281 tggattcttt gacccgggaa cttaatgtcg tttctcagca gctgttggat ctgcgccagc
     2341 aggtttctgc cctgaaggct tcctcccctc ccaatgcggt ttaaaacata aataaaaaac
     2401 cagactctgt ttggatttgg atcaagcaag tgtcttgctg tctttattta ggggttttgc
     2461 gcgcgcggta ggcccgggac cagcggtctc ggtcgttgag ggtcctgtgt attttttcca
     2521 ggacgtggta aaggtgactc tggatgttca gatacatggg cataagcccg tctctggggt
     2581 ggaggtagca ccactgcaga gcttcatgct gcggggtggt gttgtagatg atccagtcgt
     2641 agcaggagcg ctgggcgtgg tgcctaaaaa tgtctttcag tagcaagctg attgccaggg
     2701 gcaggccctt ggtgtaagtg tttacaaagc ggttaagctg ggatgggtgc atacgtgggg
     2761 atatgagatg catcttggac tgtattttta ggttggctat gttcccagcc atatccctcc
     2821 ggggattcat gttgtgcaga accaccagca cagtgtatcc ggtgcacttg ggaaatttgt
     2881 catgtagctt agaaggaaat gcgtggaaga acttggagac gcccttgtga cctccaagat
     2941 tttccatgca ttcgtccata atgatggcaa tgggcccacg ggcggcggcc tgggcgaaga
     3001 tatttctggg atcactaacg tcatagttgt gttccaggat gagatcgtca taggccattt
     3061 ttacaaagcg cgggcggagg gtgccagact gcggtataat ggttccatcc ggcccagggg
     3121 cgtagttacc ctcacagatt tgcatttccc acgctttgag ttcagatggg gggatcatgt
     3181 ctacctgcgg ggcgatgaag aaaacggttt ccggggtagg ggagatcagc tgggaagaaa
     3241 gcaggttcct gagcagctgc gacttaccgc agccggtggg cccgtaaatc acacctatta
     3301 ccgggtgcaa ctggtagtta agagagctgc agctgccgtc atccctgagc aggggggcca
     3361 cttcgttaag catgtccctg actcgcatgt tttccctgac caaatccgcc agaaggcgct
     3421 cgccgcccag cgatagcagt tcttgcaagg aagcaaagtt tttcaacggt ttgagaccgt
     3481 ccgccgtagg catgcttttg agcgtttgac caagcagttc caggcggtcc cacagctcgg
     3541 tcacctgctc tacggcatct cgatccagca tatctcctcg tttcgcgggt tggggcggct
     3601 ttcgctgtac ggcagtagtc ggtgctcgtc cagacgggcc agggtcatgt ctttccacgg
     3661 gcgcagggtc ctcgtcagcg tagtctgggt cacggtgaag gggtgcgctc cgggctgcgc
     3721 gctggccagg gtgcgcttga ggctggtcct gctggtgctg aagcgctgcc ggtcttcgcc
     3781 ctgcgcgtcg gccaggtagc atttgaccat ggtgtcatag tccagcccct ccgcggcgtg
     3841 gcccttggcg cgcagcttgc ccttggagga ggcgccgcac gaggggcagt gcagactttt
     3901 gagggcgtag agcttgggcg cgagaaatac cgattccggg gagtaggcat ccgcgccgca
     3961 ggccccgcag acggtctcgc attccacgag ccaggtgagc tctggccgtt cggggtcaaa
     4021 aaccaggttt cccccatgct ttttgatgcg tttcttacct ctggtttcca tgagccggtg
     4081 tccacgctcg gtgacgaaaa ggctgtccgt gtccccgtat acagacttga gagggagttt
     4141 aaacgaattc aatagcttgt tgcatgggcg gcgatataaa atgcaaggtg ctgctcaaaa
     4201 aatcaggcaa agcctcgcgc aaaaaagaaa gcacatcgta gtcatgctca tgcagataaa
     4261 ggcaggtaag ctccggaacc accacagaaa aagacaccat ttttctctca aacatgtctg
     4321 cgggtttctg cataaacaca aaataaaata acaaaaaaac atttaaacat tagaagcctg
     4381 tcttacaaca ggaaaaacaa cccttataag cataagacgg actacggcca tgccggcgtg
     4441 accgtaaaaa aactggtcac cgtgattaaa aagcaccacc gacagctcct cggtcatgtc
     4501 cggagtcata atgtaagact cggtaaacac atcaggttga ttcatcggtc agtgctaaaa
     4561 agcgaccgaa atagcccggg ggaatacata cccgcaggcg tagagacaac attacagccc
     4621 ccataggagg tataacaaaa ttaataggag agaaaaacac ataaacacct gaaaaaccct
     4681 cctgcctagg caaaatagca ccctcccgct ccagaacaac atacagcgct tcacagcggc
     4741 agcctaacag tcagccttac cagtaaaaaa gaaaacctat taaaaaaaca ccactcgaca
     4801 cggcaccagc tcaatcagtc acagtgtaaa aaagggccaa gtgcagagcg agtatatata
     4861 ggactaaaaa atgacgtaac ggttaaagtc cacaaaaaac acccagaaaa ccgcacgcga
     4921 acctacgccc agaaacgaaa gccaaaaaac ccacaacttc ctcaaatcgt cacttccgtt
     4981 ttcccacgtt acgtaacttc ccattttaag aaaactacaa ttcccaacac atacaagtta
     5041 ctccgcccta aaacctacgt cacccgcccc gttcccacgc cccgcgccac gtcacaaact
     5101 ccaccccctc attatcatat tggcttcaat ccaaaataag gtatattatt gatgatgtta
     5161 attaacatgc atggatccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     5221 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     5281 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     5341 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     5401 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     5461 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5521 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5581 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     5641 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5701 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5761 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5821 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     5881 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5941 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6001 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6061 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6121 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6181 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6241 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6301 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6361 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6421 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6481 ttgccattgc tgcagccatg agattatcaa aaaggatctt cacctagatc cttttcacgt
     6541 agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct
     6601 ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc
     6661 gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc
     6721 cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttctcg ccgccaagga
     6781 tctgatggcg caggggatca agctctgatc aagagacagg atgaggatcg tttcgcatga
     6841 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     6901 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     6961 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     7021 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     7081 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     7141 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     7201 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     7261 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     7321 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     7381 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     7441 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     7501 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     7561 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     7621 agttcttctg aattttgtta aaatttttgt taaatcagct cattttttaa ccaataggcc
     7681 gaaatcggca acatccctta taaatcaaaa gaatagaccg cgatagggtt gagtgttgtt
     7741 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     7801 accgtctatc agggcgatgg cccactacgt gaaccatcac ccaaatcaag ttttttgcgg
     7861 tcgaggtgcc gtaaagctct aaatcggaac cctaaaggga gcccccgatt tagagcttga
     7921 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     7981 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc gcgcttaatg
     8041 cgccgctaca gggcgcgtcc attcgccatt caggatcgaa ttaattctta at
//
