pSB1C3_I0500_LasI vector (V003462)

LOCUS       V003462                 3915 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003462
VERSION     V003462
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Boehm CR, Grant PK, Haseloff J.
  TITLE     Programmed emergence of a domain of gene expression in a bacterial
            population
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Boehm CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2016) Plant Sciences, University of Cambridge,
            Downing Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom
REFERENCE   3  (bases 1 to 3915)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3915)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-OCT-2016) Plant Sciences, University of Cambridge, Downing
            Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..879)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        906..1190
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     regulatory      1219..1230
                     /label="BBa_B0034"
                     /note="BBa_B0034"
                     /regulatory_class="ribosome_binding_site"
     RBS             1219..1230
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             1237..1839
                     /gene="lasI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Pseudomonas
                     aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 /
                     JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1). Accession#:
                     P33883"
     misc_feature    1846..1866
                     /label="BioBrick suffix"
                     /note="universal suffix for all parts"
     terminator      1867..1924
                     /label="his operon terminator"
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(2119..2707)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(2890..2984)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(3008..3664)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3665..3768)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     terminator      complement(3848..3891)
                     /label="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     misc_feature    3894..3915
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"