LOCUS       V003462                 3915 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003462
VERSION     V003462
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Boehm CR, Grant PK, Haseloff J.
  TITLE     Programmed emergence of a domain of gene expression in a bacterial
            population
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Boehm CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2016) Plant Sciences, University of Cambridge,
            Downing Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom
REFERENCE   3  (bases 1 to 3915)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3915)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-OCT-2016) Plant Sciences, University of Cambridge, Downing
            Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..879)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        906..1190
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     regulatory      1219..1230
                     /label="BBa_B0034"
                     /note="BBa_B0034"
                     /regulatory_class="ribosome_binding_site"
     RBS             1219..1230
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             1237..1839
                     /gene="lasI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Pseudomonas
                     aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 /
                     JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1). Accession#:
                     P33883"
     misc_feature    1846..1866
                     /label="BioBrick suffix"
                     /note="universal suffix for all parts"
     terminator      1867..1924
                     /label="his operon terminator"
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(2119..2707)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(2890..2984)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(3008..3664)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3665..3768)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     terminator      complement(3848..3891)
                     /label="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     misc_feature    3894..3915
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga ttgttcagat tggccgtcgt
     1261 gaggaatttg ataaaaagct gctgggcgag atgcacaaac tgcgtgcaca ggttttcaag
     1321 gagcgtaaag gttgggacgt tagcgttatt gacgaaatgg agattgatgg ctacgacgct
     1381 ctgtctccgt attatatgct gattcaggag gacaccccgg aagctcaggt atttggttgc
     1441 tggcgtatct tcgatactac gggtccgtat atgctgaaaa ataccttccc ggaactgctg
     1501 cacggtaaag aagcgccttg tagcccgcac atctgggaac tgtctcgttt cgctatcaac
     1561 tccggccaga aaggttccct gggcttttcc gattgtaccc tggaagcaat gcgtgcgctg
     1621 gcgcgctatt cactgcaaaa cgacatccag actctggtga ccgttactac tgtgggcgtt
     1681 gaaaagatga tgatccgtgc aggcctggac gttagccgtt tcggtccaca cctgaagatc
     1741 ggtatcgaac gcgcggtggc cctgcgtatc gaactgaatg caaaaaccca gatcgcactg
     1801 tatggtggtg ttctggtgga acaacgcctg gccgtttcct aataatacta gtagcggccg
     1861 ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
     1921 aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1981 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2041 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2101 ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg
     2161 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2221 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2281 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2341 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2401 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2461 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2521 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2581 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2641 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2701 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2761 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2821 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagctc
     2881 gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     2941 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
     3001 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     3061 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     3121 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     3181 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     3241 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     3301 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     3361 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     3421 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
     3481 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     3541 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     3601 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     3661 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     3721 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaac tcgagtgcca
     3781 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     3841 aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg
     3901 cggccgcttc tagag
//
