pSB156 vector (V003463)

LOCUS       40924_38718        6689 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSB156, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Himmelbach A, Zierold U, Hensel G, Riechen J, Douchkov D, Schweizer 
            P, Kumlehn J.
  TITLE     A set of modular binary vectors for transformation of cereals
  JOURNAL   Plant Physiol. 145 (4), 1192-1200 (2007)
  PUBMED    17981986
REFERENCE   2  (bases 1 to 6689)
  AUTHORS   Himmelbach A, Zierold U, Hensel G, Riechen J, Douchkov D, Schweizer 
            P, Kumlehn J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2007) Cytogenetics and Molecular Cell Biology, 
            Leibniz Institute of Plant Genetics and Crop Plant Research, 
            Corrensstr. 3, Gatersleben D-06466, Germany
REFERENCE   3  (bases 1 to 6689)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6689)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages: 
            "1192-1200"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-SEP-2007) Cytogenetics and Molecular Cell Biology, Leibniz 
            Institute of Plant Genetics and Crop Plant Research, Corrensstr. 3, 
            Gatersleben D-06466, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    97..118
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        133..163
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    171..187
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     195..211
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        232..250
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        317..1149
                     /label=Act1 promoter
                     /note="rice actin promoter"
     CDS             1152..1169
                     /label=6xHis
                     /note="6xHis affinity tag"
     intron          2120..2308
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     terminator      3809..4061
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      4072..4081
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4110..4128)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4135..4151)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(4292..4747)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4774..4878
                     /label=AmpR promoter
     CDS             4879..5736
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5910..6498
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"