LOCUS       40924_38718        6689 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSB156, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Himmelbach A, Zierold U, Hensel G, Riechen J, Douchkov D, Schweizer 
            P, Kumlehn J.
  TITLE     A set of modular binary vectors for transformation of cereals
  JOURNAL   Plant Physiol. 145 (4), 1192-1200 (2007)
  PUBMED    17981986
REFERENCE   2  (bases 1 to 6689)
  AUTHORS   Himmelbach A, Zierold U, Hensel G, Riechen J, Douchkov D, Schweizer 
            P, Kumlehn J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2007) Cytogenetics and Molecular Cell Biology, 
            Leibniz Institute of Plant Genetics and Crop Plant Research, 
            Corrensstr. 3, Gatersleben D-06466, Germany
REFERENCE   3  (bases 1 to 6689)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6689)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages: 
            "1192-1200"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-SEP-2007) Cytogenetics and Molecular Cell Biology, Leibniz 
            Institute of Plant Genetics and Crop Plant Research, Corrensstr. 3, 
            Gatersleben D-06466, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    97..118
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        133..163
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    171..187
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     195..211
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        232..250
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        317..1149
                     /label=Act1 promoter
                     /note="rice actin promoter"
     CDS             1152..1169
                     /label=6xHis
                     /note="6xHis affinity tag"
     intron          2120..2308
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     terminator      3809..4061
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      4072..4081
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4110..4128)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4135..4151)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(4292..4747)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4774..4878
                     /label=AmpR promoter
     CDS             4879..5736
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5910..6498
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
       61 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      121 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      181 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
      241 tcactaaagg gaacaaaagc tggagctctc tagaggccct taaggcctta ctagtggatc
      301 ccccgggact agtggctcga ggtcattcat atgcttgaga agagagtcgg gatagtccaa
      361 aataaaacaa aggtaagatt acctggtcaa aagtgaaaac atcagttaaa aggtggtata
      421 agtaaaatat cggtaataaa aggtggccca aagtgaaatt tactcttttc tactattata
      481 aaaattgagg atgttttgtc ggtactttga tacgtcattt ttgtatgaat tggtttttaa
      541 gtttattcgc gatttggaaa tgcatatctg tatttgagtc ggtttttaag ttcgttgctt
      601 ttgtaaatac agagggattt gtataagaaa tatctttaaa aaacccatat gctaatttga
      661 cataattttt gagaaaaata tatattcagg cgaattccac aatgaacaat aataagatta
      721 aaatagcttg cccccgttgc agcgatgggt attttttcta gtaaaataaa agataaactt
      781 agactcaaaa catttacaaa aacaacccct aaagtcctaa agcccaaagt gctatgcacg
      841 atccatagca agcccagccc aacccaaccc aacccaaccc accccagtgc agccaactgg
      901 caaatagtct ccacccccgg cactatcacc gtgagttgtc cgcaccaccg cacgtctcgc
      961 agccaaaaaa aaaaaaagaa agaaaaaaaa gaaaaagaaa aacagcaggt gggtccgggt
     1021 cgtgggggcc ggaaaagcga ggaggatcgc gagcagcgac gaggcccggc cctccctccg
     1081 cttccaaaga aacgcccccc atcgccacta tatacatacc cccccctctc ctcccatccc
     1141 cccaacccta ccaccaccac caccaccacc tcctcccccc tcgctgccgg acgacgagct
     1201 cctcccccct ccccctccgc cgccgccggt aaccaccccg cccctctcct ctttctttct
     1261 ccgttttttt tttcgtctcg gtctcgatct ttggccttgg tagtttgggt gggcgagagc
     1321 ggcttcgtcg cccagatcgg tgcgcgggag gggcgggatc tcgcggctgg cgtctccggg
     1381 cgtgagtcgg cccggatcct cgcggggaat ggggctctcg gatgtagatc tgcgatccgc
     1441 cgttgttggg ggagatgatg gggggtttaa aatttccgcc atgctaaaca agatcaggaa
     1501 gaggggaaaa gggcactatg gtttatattt ttatatattt ctgctgcttc gtcaggctta
     1561 gatgtgctag atcttctttc tttcttcttt ttgtggtaga atttgaatcc ctcagcattg
     1621 ttcatcggta gtttttcttt tcatgatttg tgacaaatgc agcctcgtgc ggagcttttt
     1681 tgtaggcgcg gggctgcagg aattcaagct tacgcgtgtc gacgtcagtc ccttatgtta
     1741 cgtcctgtag aaaccccaac ccgtgaaatc aaaaaactcg acggcctgtg ggcattcagt
     1801 ctggatcgcg aaaactgtgg aattggtcag cgttggtggg aaagcgcgtt acaagaaagc
     1861 cgggcaattg ctgtgccagg cagttttaac gatcagttcg ccgatgcaga tattcgtaat
     1921 tatgcgggca acgtctggta tcagcgcgaa gtctttatac cgaaaggttg ggcaggccag
     1981 cgtatcgtgc tgcgtttcga tgcggtcact cattacggca aagtgtgggt caataatcag
     2041 gaagtgatgg agcatcaggg cggctatacg ccatttgaag ccgatgtcac gccgtatgtt
     2101 attgccggga aaagtgtacg taagtttctg cttctacctt tgatatatat ataataatta
     2161 tcattaatta gtagtaatat aatatttcaa atattttttt caaaataaaa gaatgtagta
     2221 tatagcaatt gcttttctgt agtttataag tgtgtatatt ttaatttata acttttctaa
     2281 tatatgacca aaatttgttg atgtgcagct ggtatcaccg tttgtgtgaa caacgaactg
     2341 aactggcaga ctatcccgcc gggaatggtg attaccgacg aaaacggcaa gaaaaagcag
     2401 tcttacttcc atgatttctt taactatgcc ggaatccatc gcagcgtaat gctctacacc
     2461 acgccgaaca cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca agactgtaac
     2521 cacgcgtctg ttgactggca ggtggtggcc aatggtgatg tcagcgttga actgcgtgat
     2581 gcggatcaac aggtggttgc aactggacaa ggcactagcg ggactttgca agtggtgaat
     2641 ccgcacctct ggcaaccggg tgaaggttat ctctatgaac tgtgcgtcac agccaaaagc
     2701 cagacagagt gtgatatcta cccgcttcgc gtcggcatcc ggtcagtggc agtgaagggc
     2761 caacagttcc tgattaacca caaaccgttc tactttactg gctttggtcg tcatgaagat
     2821 gcggacttgc gtggcaaagg attcgataac gtgctgatgg tgcacgacca cgcattaatg
     2881 gactggattg gggccaactc ctaccgtacc tcgcattacc cttacgctga agagatgctc
     2941 gactgggcag atgaacatgg catcgtggtg attgatgaaa ctgctgctgt cggctttaac
     3001 ctctctttag gcattggttt cgaagcgggc aacaagccga aagaactgta cagcgaagag
     3061 gcagtcaacg gggaaactca gcaagcgcac ttacaggcga ttaaagagct gatagcgcgt
     3121 gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca acgaaccgga tacccgtccg
     3181 caaggtgcac gggaatattt cgcgccactg gcggaagcaa cgcgtaaact cgacccgacg
     3241 cgtccgatca cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac catcagcgat
     3301 ctctttgatg tgctgtgcct gaaccgttat tacggatggt atgtccaaag cggcgatttg
     3361 gaaacggcag agaaggtact ggaaaaagaa cttctggcct ggcaggagaa actgcatcag
     3421 ccgattatca tcaccgaata cggcgtggat acgttagccg ggctgcactc aatgtacacc
     3481 gacatgtgga gtgaagagta tcagtgtgca tggctggata tgtatcaccg cgtctttgat
     3541 cgcgtcagcg ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc gacctcgcaa
     3601 ggcatattgc gcgttggcgg taacaagaaa gggatcttca ctcgcgaccg caaaccgaag
     3661 tcggcggctt ttctgctgca aaaacgctgg actggcatga acttcggtga aaaaccgcag
     3721 cagggaggca aacaatgaat caacaactct cctggcgcac catcgtcggc tacagcctcg
     3781 ggaattgcta ccgagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt
     3841 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     3901 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     3961 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     4021 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgctcgacgc ggccgccatg
     4081 gccagatctg ggcccggtac ccaattcgcc ctatagtgag tcgtattaca attcactggc
     4141 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     4201 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     4261 ccaacagttg cgcagcctga atggcgaatg gaaattgtaa gcgttaatat tttgttaaaa
     4321 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     4381 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     4441 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     4501 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     4561 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     4621 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     4681 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     4741 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     4801 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     4861 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     4921 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     4981 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5041 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5101 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5161 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5221 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5281 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5341 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5401 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5461 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5521 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5581 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     5641 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     5701 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     5761 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5821 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5881 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5941 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6001 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     6061 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6121 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6181 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6241 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6301 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6361 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6421 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6481 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6541 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6601 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     6661 gtgagcgagg aagcggaaga gcgcccaat
//
