Basic Vector Information
pTn7xK vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTn7xK vector Sequence
LOCUS 40924_43713 4208 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pTn7xK, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4208)
AUTHORS Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP.
TITLE Tn5/7-lux: A versatile tool for the identification and capture of
promoters in Gram-negative bacteria
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4208)
AUTHORS Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP.
TITLE Direct Submission
JOURNAL Submitted (04-NOV-2013) Microbiology, Immunology and Pathology,
Colorado State University, 2025 Campus Delivery, Fort Collins, CO
80523, USA
REFERENCE 3 (bases 1 to 4208)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4208)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(04-NOV-2013) Microbiology, Immunology and Pathology, Colorado State
University, 2025 Campus Delivery, Fort Collins, CO 80523, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4208
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 58..105
/label=FRT site
/note="FRT site"
protein_bind complement(58..105)
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
CDS 491..1282
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
protein_bind complement(1384..1431)
/label=FRT
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
terminator complement(1477..1563)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator complement(1666..1760)
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
primer_bind complement(1788..1804)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
repeat_region 1809..2008
/note="Tn7R; Tn7 right end"
oriT 2753..2861
/label=oriT
/note="incP origin of transfer"
rep_origin 2884..3268
/label=R6K gamma ori
/note="gamma replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication"
mobile_element 3484..3649
/label=Tn7L
/note="mini-Tn7 element (left end of the Tn7 transposon)"
protein_bind 3680..3704
/label=attB1
/note="recombination site for the Gateway(R) BP reaction"
regulatory 3735..4200
/label=Pseudomonas aeruginosa PA4974 gene
/note="Pseudomonas aeruginosa PA4974 gene"
/regulatory_class="promoter"
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