LOCUS       40924_43713        4208 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTn7xK, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Tn5/7-lux: A versatile tool for the identification and capture of 
            promoters in Gram-negative bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2013) Microbiology, Immunology and Pathology, 
            Colorado State University, 2025 Campus Delivery, Fort Collins, CO 
            80523, USA
REFERENCE   3  (bases 1 to 4208)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4208)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-NOV-2013) Microbiology, Immunology and Pathology, Colorado State
            University, 2025 Campus Delivery, Fort Collins, CO 80523, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    58..105
                     /label=FRT site
                     /note="FRT site"
     protein_bind    complement(58..105)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             491..1282
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     protein_bind    complement(1384..1431)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(1477..1563)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(1666..1760)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1788..1804)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1809..2008
                     /note="Tn7R; Tn7 right end"
     oriT            2753..2861
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2884..3268
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     mobile_element  3484..3649
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     protein_bind    3680..3704
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3735..4200
                     /label=Pseudomonas aeruginosa PA4974 gene
                     /note="Pseudomonas aeruginosa PA4974 gene"
                     /regulatory_class="promoter"
ORIGIN
        1 gcccggggga tccactagtg agctcatgca tgatcgaatt agcttcaaaa gcgctctgaa
       61 gttcctatac tttctagaga ataggaactt cggaatagga acttcaagat cccctgattc
      121 cctttgtcaa cagcaatgga taattcgatt aacaaatgca tggcgcaagg gctgctaaag
      181 gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag
      241 ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag
      301 tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt
      361 gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt
      421 cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga caggatgagg
      481 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
      541 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
      601 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
      661 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
      721 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
      781 gccggggcag gatctcctgt catcccacct tgctcctgcc gagaaagtat ccatcatggc
      841 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
      901 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
      961 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     1021 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     1081 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     1141 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     1201 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     1261 tcgccttctt gacgagttct tctgaattga aaaaggaaga gtatgaggat ccaacatttc
     1321 caatcactta attaattatt ccattgagta agtttttaag cacatcagct tcaaaagcgc
     1381 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggtactt caagatcccc
     1441 aattcgatcg tccgggccgc aagctcctag cggcggattt gtcctactca ggagagcgtt
     1501 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
     1561 ttgatgcctc aagctagaga gtcattaccc caggcgttta agggcaccaa taactgcctt
     1621 aaaaaaatta cgccccgccc tgccactcat cgcagtctag cttggattct caccaataaa
     1681 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     1741 tggatctatc aacaggagtc caagctcagc taattaagct agcttatcga taccgtcgac
     1801 ctcgaacccc acgcccctct ttaatacgac gggcaatttg cacttcagaa aatgaagagt
     1861 ttgctttagc cataacaaaa gtccagtatg ctttttcaca gcataactgg actgatttca
     1921 gtttacaact attctgtcta gtttaagact ttattgtcat agtttagatc tattttgttc
     1981 agtttaagac tttattgtcc gcccacaccc gcttacgcag ggcatccatt tattactcaa
     2041 ccgtaaccga ttttgccagg ttacgcggct ggtcctctag ctggcgtaat agcgaagagg
     2101 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     2161 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     2221 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     2281 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     2341 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     2401 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     2461 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     2521 caaatatgta tccggggctt ccgcttcctc gctcactgac ccgctgcgct cggtcgttcg
     2581 gctgcggcga gcggtatcag gggattcctt aaggtatact ttccgctgca taaccctgct
     2641 tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat
     2701 tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga
     2761 taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc
     2821 tggcggtgct caacgggaat cctgctctgc gaggctggcc gataagctct gataacgcag
     2881 gaaagaacat gggcagttca acctgttgat agtacgtact aagctctcat gtttcacgta
     2941 ctaagctctc aagtttaacg tactaagctc tcatgtttaa cgaactaaac cctcatggct
     3001 aacgtactaa gctctcatgg ctaacgtact aagctctcat gtttcacgta ctaagctctc
     3061 atgtttgaac aataaaatta atataaatca gcaacttaaa tagcctctaa ggttttaagt
     3121 tttataagaa aaaaaagaat atataaggct tttaaagctt ttaaggttta acggttgtgg
     3181 acaacaagcc agggatgtaa cgcactgaga agcccttaga gcctctcaaa gcaattttga
     3241 gtgacacagg aacacttaac ggctgacaac cggctggtaa caggattagc agagcgaggt
     3301 atgtaggcgg tgctacagag ttcttgaagt ggtggcctcc cctcgccgca gccgaacgac
     3361 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     3421 ccccgcgcgt tggccgattc attaatgcag cgcccaaaca taacaggaag aaaaatgccc
     3481 cgctgtgggc ggacaaaata gttgggaact gggaggggtg gaaatggagt ttttaaggat
     3541 tatttaggga agagtgacaa aatagatggg aactgggtgt agcgtcgtaa gctaatacga
     3601 aaattaaaaa tgacaaaata gtttggaact agatttcact tatctggttg gcctgcaagg
     3661 ccttcgcgag gtacccatca caagtttgta caaaaaagca ggctttaatt aatcgaggcg
     3721 cgccatcgca ctatgtgatc gatggctctc ctgggtgatt gtcgggagaa cctggcacgc
     3781 ggggccaccg ggactggtgg cgagaaggac gcgccggcaa ggcaggcatg gcgcggacat
     3841 cttgttccct acgcgggttg ctgcggtccg cgcgaggcgg aaccggacgc tagcccgatc
     3901 aggttcaacg ggatccggaa ctatccgatc tcagcccctc gtatagggca ccccgacaag
     3961 aaccccggca gtctaatcaa caccggcggg atacgccaac gggaaaacgc cccgcccgac
     4021 cggcagtcat ggtccagtcc ggcgccctct cgccagggaa gacgcagatc cccgcacgtt
     4081 cgccggcaca tcgatccgga ctggctcttc gatcagttgc cgcgcaggtg ccttgaccgc
     4141 caccggtagc gaccttagcc ttgccccacc tcgcatcttt tccaggacga acgacacgcg
     4201 gtgctgca
//
