Basic Vector Information
- Vector Name:
- pTn5.7luxT5
- Antibiotic Resistance:
- Trimethoprim
- Length:
- 10774 bp
- Type:
- Cloning vector
- Replication origin:
- R6K γ ori
- Source/Author:
- Kvtiko BH, Bruckbauer ST, Karkhoff-Schweizer RR, Schweizer HP.
- Promoter:
- Pc
pTn5.7luxT5 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTn5.7luxT5 vector Sequence
LOCUS 40924_43653 10774 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pTn5.7luxT5, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10774)
AUTHORS Kvtiko BH, Bruckbauer ST, Karkhoff-Schweizer RR, Schweizer HP.
TITLE Tn5/7 lux: A versatile vector for the location, capture, and
utilization of Gram negative promoters
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 10774)
AUTHORS Kvtiko BH, Bruckbauer ST, Karkhoff-Schweizer RR, Schweizer HP.
TITLE Direct Submission
JOURNAL Submitted (09-AUG-2013) Microbiology Immunology and Pathology,
Colorado State University, IDRC at Foothills Campus, Campus Delivery
0922, Fort Collins, CO 80523-0922, USA
REFERENCE 3 (bases 1 to 10774)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 10774)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(09-AUG-2013) Microbiology Immunology and Pathology, Colorado State
University, IDRC at Foothills Campus, Campus Delivery 0922, Fort
Collins, CO 80523-0922, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..10774
/mol_type="other DNA"
/organism="synthetic DNA construct"
oriT 365..473
/label=oriT
/note="incP origin of transfer"
rep_origin 496..880
/label=R6K gamma ori
/note="gamma replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication"
mobile_element 1096..1261
/label=Tn7L
/note="mini-Tn7 element (left end of the Tn7 transposon)"
misc_feature complement(1276..1294)
/label=Tn5 ME
/note="hyperactive mosaic end for Tn5 transposase
recognition (Reznikoff et al., 2004)"
promoter complement(1295..1399)
/label=AmpR promoter
CDS complement(1449..2876)
/label=Tn5 transposase
/note="transposase from the bacterial Tn5 transposon
(Reznikoff, 1993)"
regulatory complement(3019..3048)
/gene="tnpA"
/label=S12 promoter
/note="S12 promoter"
/regulatory_class="promoter"
misc_feature 3055..3073
/label=Tn5 ME
/note="hyperactive mosaic end for Tn5 transposase
recognition (Reznikoff et al., 2004)"
CDS 3106..4545
/label=LuxC
/note="LuxC fatty acid reductase"
CDS 4560..5480
/label=LuxD
/note="LuxD acyltransferase"
CDS 5532..6611
/label=LuxA
/note="LuxA luciferase subunit"
CDS 6629..7609
/label=LuxB
/note="LuxB luciferase subunit"
CDS 7791..8900
/label=LuxE
/note="LuxE"
protein_bind complement(8938..8962)
/label=attB2
/note="recombination site for the Gateway(R) BP reaction"
misc_recomb 9018..9065
/label=FRT
/note="FRT"
protein_bind complement(9018..9065)
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
promoter 9117..9145
/label=Pc promoter
/note="class 1 integron promoter"
CDS 9472..9705
/label=TpR
/note="E. coli plasmid-associated dihydrofolate reductase"
protein_bind complement(9770..9817)
/label=FRT
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
terminator complement(9863..9949)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator complement(10052..10146)
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
primer_bind complement(10174..10190)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 10195..10393
/label=Tn7R
/note="Tn7R"
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