LOCUS       40924_43653       10774 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTn5.7luxT5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10774)
  AUTHORS   Kvtiko BH, Bruckbauer ST, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Tn5/7 lux: A versatile vector for the location, capture, and 
            utilization of Gram negative promoters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10774)
  AUTHORS   Kvtiko BH, Bruckbauer ST, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2013) Microbiology Immunology and Pathology, 
            Colorado State University, IDRC at Foothills Campus, Campus Delivery
            0922, Fort Collins, CO 80523-0922, USA
REFERENCE   3  (bases 1 to 10774)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10774)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-AUG-2013) Microbiology Immunology and Pathology, Colorado State 
            University, IDRC at Foothills Campus, Campus Delivery 0922, Fort 
            Collins, CO 80523-0922, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            365..473
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      496..880
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     mobile_element  1096..1261
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     misc_feature    complement(1276..1294)
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     promoter        complement(1295..1399)
                     /label=AmpR promoter
     CDS             complement(1449..2876)
                     /label=Tn5 transposase
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"
     regulatory      complement(3019..3048)
                     /gene="tnpA"
                     /label=S12 promoter
                     /note="S12 promoter"
                     /regulatory_class="promoter"
     misc_feature    3055..3073
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     CDS             3106..4545
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
     CDS             4560..5480
                     /label=LuxD
                     /note="LuxD acyltransferase"
     CDS             5532..6611
                     /label=LuxA
                     /note="LuxA luciferase subunit"
     CDS             6629..7609
                     /label=LuxB
                     /note="LuxB luciferase subunit"
     CDS             7791..8900
                     /label=LuxE
                     /note="LuxE"
     protein_bind    complement(8938..8962)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     9018..9065
                     /label=FRT
                     /note="FRT"
     protein_bind    complement(9018..9065)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        9117..9145
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             9472..9705
                     /label=TpR
                     /note="E. coli plasmid-associated dihydrofolate reductase"
     protein_bind    complement(9770..9817)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(9863..9949)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(10052..10146)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(10174..10190)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    10195..10393
                     /label=Tn7R
                     /note="Tn7R"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccggggc ttccgcttcc tcgctcactg acccgctgcg
      181 ctcggtcgtt cggctgcggc gagcggtatc aggggattcc ttaaggtata ctttccgctg
      241 cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac
      301 gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc
      361 agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc
      421 cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgataagct
      481 ctgataacgc aggaaagaac atgggcagtt caacctgttg atagtacgta ctaagctctc
      541 atgtttcacg tactaagctc tcaagtttaa cgtactaagc tctcatgttt aacgaactaa
      601 accctcatgg ctaacgtact aagctctcat ggctaacgta ctaagctctc atgtttcacg
      661 tactaagctc tcatgtttga acaataaaat taatataaat cagcaactta aatagcctct
      721 aaggttttaa gttttataag aaaaaaaaga atatataagg cttttaaagc ttttaaggtt
      781 taacggttgt ggacaacaag ccagggatgt aacgcactga gaagccctta gagcctctca
      841 aagcaatttt gagtgacaca ggaacactta acggctgaca accggctggt aacaggatta
      901 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct cccctcgccg
      961 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     1021 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcgcccaaa cataacagga
     1081 agaaaaatgc cccgctgtgg gcggacaaaa tagttgggaa ctgggagggg tggaaatgga
     1141 gtttttaagg attatttagg gaagagtgac aaaatagatg ggaactgggt gtagcgtcgt
     1201 aagctaatac gaaaattaaa aatgacaaaa tagtttggaa ctagatttca cttatctggt
     1261 tggcctgcaa ggcctagatg tgtataagag acagactctt cctttttcaa tattattgaa
     1321 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1381 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgcagat ctgcaggtcg
     1441 accgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt
     1501 ttactttgca gggcttccca accttcccag agggcgcccc agctggcaat tccggttcgc
     1561 ttgctgtcca taaaaccgcc cagtctagct atcgccatgt aagcccactg caagctacct
     1621 gctttctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc
     1681 ggggtcagca ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt tagcagccct
     1741 tgcgccctga gtgcttgcgg cagcgtgaag ctttctctga gctgtaacag cctgaccgca
     1801 acaaacgaga ggatcgagac catccgctcc agattatccg gctcctccat gcgttgcctc
     1861 tcggctcctg ctccggtttt ccatgcctta tggaactcct cgatccgcca gcgatgggta
     1921 taaatgtcga tgacgcgcaa ggcttgggct agcgactcga ccggttcgct ggtcagcaac
     1981 aaccatttca acggggtctc acccttgggc gggttaatct cctcggccag caccgcgttg
     2041 agcgtgatat tcccctgttt tagcgtgatg cgcccactgc gcaggctcaa gctcgccttg
     2101 cgggctggtc gatttttacg tttaccgcgt ttatccacca cgcccttttg cggaatgctg
     2161 atctgatagc cacccaactc cggttggttc ttcagatggt cgtacagata caacccagac
     2221 tctacgtcct tgcgtgggtg cttggagcgc accacgaagc gctcgttatg cgccagtttg
     2281 tcctgcagat aagcatgaat atcggcttcg cggtcacaga ccgcaatcac gttgctcatc
     2341 atgctgccca tgcgtaaccg gctagttgcg gccgctgcca gccatttgcc actctccttt
     2401 tcatccgcat cggcagggtc atccgggcgc atccaccact cctgatgcag taatcctacg
     2461 gtgcggaatg tggtggcctc gagcaagaga acggagtgaa tccaccatcc gcgggattta
     2521 tcctgaatag agcccagctt gccaagctct tcggcgacct ggtggcgata actcaaagag
     2581 gtggtgtcct caatggccag cagttcggga aactcctgag ccaacttgac tgtttgcatg
     2641 gcgccagcct ttctgatcgc ctcggcagaa acgttgggat tgcggataaa tcggtaagcg
     2701 ccttcctggg cggcttcact accctctgat gagatgggta ttgatttacc agaatatttt
     2761 gccaattggg cggcgacgtt aaccaagcgg gcagtatggc gaggatcacc cagcgccgcc
     2821 gaagagaaca cagatttagc ccagtcggcc gcacgatgaa gagcagaagt tatcatgaac
     2881 gttaccatgt taggaggtca catggcagat cagatcctgg aaaacgggaa aggttccgtt
     2941 caggacgcta cttgtgtacg ggatcggtcg acggatccca agcttcttct agtaacggcc
     3001 gccagtgtgc tggaattcat gatattccga aaatcccaag tgagtcaaga attcctgtct
     3061 cttatacaca tctcacgcgg tgcattaaat ggatggcaaa tatgaatgac taaaaaaatt
     3121 tcattcatta ttaacggcca ggttgaaatc tttcccgaaa gtgatgattt agtgcaatcc
     3181 attaattttg gtgataatag tgtttacctg ccaatattga atgactctca tgtaaaaaac
     3241 attattgatt gtaatggaaa taacgaatta cggttgcata acattgtcaa ttttctctat
     3301 acggtagggc aaagatggaa aaatgaagaa tactcaagac gcaggacata cattcgtgac
     3361 ttaaaaaaat atatgggata ttcagaagaa atggctaagc tagaggccaa ttggatatct
     3421 atgattttat gttctaaagg cggcctttat gatgttgtag aaaatgaact tggttctcgc
     3481 catatcatgg atgaatggct acctcaggat gaaagttatg ttcgggcttt tccgaaaggt
     3541 aaatctgtac atctgttggc aggtaatgtt ccattatctg ggatcatgtc tatattacgc
     3601 gcaattttaa ctaagaatca gtgtattata aaaacatcgt caaccgatcc ttttaccgct
     3661 aatgcattag cgttaagttt tattgatgta gaccctaatc atccgataac gcgctcttta
     3721 tctgttatat attggcccca ccaaggtgat acatcactcg caaaagaaat tatgcgacat
     3781 gcggatgtta ttgtcgcttg gggagggcca gatgcgatta attgggcggt agagcacgcg
     3841 ccatcttatg ctgatgtgat taaatttggt tctaaaaaga gtctttgcat tatcgataat
     3901 cctgttgatt tgacgtccgc agcgacaggt gcggctcatg atgtttgttt ttacgatcag
     3961 cgagcttgtt tttctgccca aaacatatat tacatgggaa atcattatga ggaatttaag
     4021 ttagcgttga tagaaaaact taatctatat gcgcatatat taccgaatgc caaaaaagat
     4081 tttgatgaaa aggcggccta ttctttagtt caaaaagaaa gcctgtttgc tggattaaaa
     4141 gtagaggtgg atattcatca acgttggatg attattgagt caaatgcagg tgtggaattt
     4201 aatcaaccac ttggcagatg tgtgtacctt catcacgtcg ataatattga gcaaatattg
     4261 ccttatgttc aaaaaaataa gacgcaaacc atatctattt ttccttggga gtcatcattt
     4321 aaatatcgag atgcgttagc attaaaaggt gcggaaagga ttgtagaagc aggaatgaat
     4381 aacatatttc gagttggtgg atctcatgac ggaatgagac cgttgcaacg attagtgaca
     4441 tatatttctc atgaaaggcc atctaactat acggctaagg atgttgcggt tgaaatagaa
     4501 cagactcgat tcctggaaga agataagttc cttgtatttg tcccataata ggtaaaagta
     4561 tggaaaatga atcaaaatat aaaaccatcg accacgttat ttgtgttgaa ggaaataaaa
     4621 aaattcatgt ttgggaaacg ctgccagaag aaaacagccc aaagagaaag aatgccatta
     4681 ttattgcgtc tggttttgcc cgcaggatgg atcattttgc tggtctggcg gaatatttat
     4741 cgcggaatgg atttcatgtg atccgctatg attcgcttca ccacgttgga ttgagttcag
     4801 ggacaattga tgaatttaca atgtctatag gaaagcagag cttgttagca gtggttgatt
     4861 ggttaactac acgaaaaata aataacttcg gtatgttggc ttccagctta tctgcgcgga
     4921 tagcttatgc aagcctatct gaaatcaatg cttcgttttt aatcaccgca gtcggtgttg
     4981 ttaacttaag atattctctt gaaagagctt tagggtttga ttatctcagt ctacccatta
     5041 atgaattgcc ggataatctg gattttgaag gccataaatt gggtgctgaa gtctttgcga
     5101 gagattgtct tgattttggt tgggaagatt tagcttctac aattaataac atgatgtatc
     5161 ttgatatacc gtttattgct tttactgcaa ataacgataa ttgggtcaag caagatgaag
     5221 ttatcacatt gttatcaaat attcgtagta atcgatgcaa gatatattct ttgttaggaa
     5281 gttcgcatga cttgagtgaa aatttagtgg tcctgcgcaa tttttatcaa tcggttacga
     5341 aagccgctat cgcgatggat aatgatcatc tggatattga tgttgatatt actgaaccgt
     5401 catttgaaca tttaactatt gcgacagtca atgaacgccg aatgagaatt gagattgaaa
     5461 atcaagcaat ttctctgtct taaaatctat tgagatattc tatcactcaa atagcaatat
     5521 aaggactctc tatgaaattt ggaaactttt tgcttacata ccaacctccc caattttctc
     5581 aaacagaggt aatgaaacgt ttggttaaat taggtcgcat ctctgaggag tgtggttttg
     5641 ataccgtatg gttactggag catcatttca cggagtttgg tttgcttggt aacccttatg
     5701 tcgctgctgc atatttactt ggcgcgacta aaaaattgaa tgtaggaact gccgctattg
     5761 ttcttcccac agcccatcca gtacgccaac ttgaagatgt gaatttattg gatcaaatgt
     5821 caaaaggacg atttcggttt ggtatttgcc gagggcttta caacaaggac tttcgcgtat
     5881 tcggcacaga tatgaataac agtcgcgcct tagcggaatg ctggtacggg ctgataaaga
     5941 atggcatgac agagggatat atggaagctg ataatgaaca tatcaagttc cataaggtaa
     6001 aagtaaaccc cgcggcgtat agcagaggtg gcgcaccggt ttatgtggtg gctgaatcag
     6061 cttcgacgac tgagtgggct gctcaatttg gcctaccgat gatattaagt tggattataa
     6121 atactaacga aaagaaagca caacttgagc tttataatga agtggctcaa gaatatgggc
     6181 acgatattca taatatcgac cattgcttat catatataac atctgtagat catgactcaa
     6241 ttaaagcgaa agagatttgc cggaaatttc tggggcattg gtatgattct tatgtgaatg
     6301 ctacgactat ttttgatgat tcagaccaaa caagaggtta tgatttcaat aaagggcagt
     6361 ggcgtgactt tgtattaaaa ggacataaag atactaatcg ccgtattgat tacagttacg
     6421 aaatcaatcc cgtgggaacg ccgcaggaat gtattgacat aattcaaaaa gacattgatg
     6481 ctacaggaat atcaaatatt tgttgtggat ttgaagctaa tggaacagta gacgaaatta
     6541 ttgcttccat gaagctcttc cagtctgatg tcatgccatt tcttaaagaa aaacaacgtt
     6601 cgctattata ttagctaagg agaaagaaat gaaatttgga ttgttcttcc ttaacttcat
     6661 caattcaaca actgttcaag aacaaagtat agttcgtatg caggaaataa cggagtatgt
     6721 tgataagttg aattttgaac agattttagt gtatgaaaat catttttcag ataatggtgt
     6781 tgtcggcgct cctctgactg tttctggttt tctgctcggt ttaacagaga aaattaaaat
     6841 tggttcatta aatcacatca ttacaactca tcatcctgtc gccatagcgg aggaagcgtg
     6901 cttattggat cagttaagtg aagggagatt tattttaggg tttagtgatt gcgaaaaaaa
     6961 agatgaaatg cattttttta atcgcccggt tgaatatcaa cagcaactat ttgaagagtg
     7021 ttatgaaatc attaacgatg ctttaacaac aggctattgt aatccagata acgattttta
     7081 tagcttccct aaaatatctg taaatcccca tgcttatacg ccaggcggac ctcggaaata
     7141 tgtaacagca accagtcatc atattgttga gtgggcggcc aaaaaaggta ttcctctcat
     7201 ctttaagtgg gatgattcta atgatgttag atatgaatat gctgaaagat ataaagccgt
     7261 tgcggataaa tatgacgttg acctatcaga gatagaccat cagttaatga tattagttaa
     7321 ctataacgaa gatagtaata aagctaaaca agagacgcgt gcatttatta gtgattatgt
     7381 tcttgaaatg caccctaatg aaaatttcga aaataaactt gaagaaataa ttgcagaaaa
     7441 cgctgtcgga aattatacgg agtgtataac tgcggctaag ttggcaattg aaaagtgtgg
     7501 tgcgaaaagt gtattgctgt cctttgaacc aatgaatgat ttgatgagcc aaaaaaatgt
     7561 aatcaatatt gttgatgata atattaagaa gtaccacatg gaatatacct aatagatttc
     7621 gagttgcagc gaggcggcaa gtgaacgaat ccccaggagc atagataact atgtgactgg
     7681 ggtgagtgaa agcagccaac aaagcagcag cttgaaagat gaagggtata aaagagtatg
     7741 acagcagtgc tgccatactt tctaatatta tcttgaggag taaaacaggt atgacttcat
     7801 atgttgataa acaagaaatt acagcaagct cagaaattga tgatttgatt ttttcgagcg
     7861 atccattagt gtggtcttac gacgagcagg aaaaaatcag aaagaaactt gtgcttgatg
     7921 catttcgtaa tcattataaa cattgtcgag aatatcgtca ctactgtcag gcacacaaag
     7981 tagatgacaa tattacggaa attgatgaca tacctgtatt cccaacatcg gtttttaagt
     8041 ttactcgctt attaacttct caggaaaacg agattgaaag ttggtttacc agtagcggca
     8101 cgaatggttt aaaaagtcag gtggcgcgtg acagattaag tattgagaga ctcttaggct
     8161 ctgtgagtta tggcatgaaa tatgttggta gttggtttga tcatcaaata gaattagtca
     8221 atttgggacc agatagattt aatgctcata atatttggtt taaatatgtt atgagtttgg
     8281 tggaattgtt atatcctacg acatttaccg taacagaaga acgaatagat tttgttaaaa
     8341 cattgaatag tcttgaacga ataaaaaatc aagggaaaga tctttgtctt attggttcgc
     8401 catactttat ttatttactc tgccattata tgaaagataa aaaaatctca ttttctggag
     8461 ataaaagcct ttatatcata accggaggcg gctggaaaag ttacgaaaaa gaatctctga
     8521 aacgtgatga tttcaatcat cttttatttg atactttcaa tctcagtgat attagtcaga
     8581 tccgagatat atttaatcaa gttgaactca acacttgttt ctttgaggat gaaatgcagc
     8641 gtaaacatgt tccgccgtgg gtatatgcgc gagcgcttga tcctgaaacg ttgaaacctg
     8701 tacctgatgg aacgccgggg ttgatgagtt atatggatgc gtcagcaacc agttatccag
     8761 catttattgt taccgatgat gtcgggataa ttagcagaga atatggtaag tatcccggcg
     8821 tgctcgttga aattttacgt cgcgtcaata cgaggacgca gaaagggtgt gctttgagct
     8881 taaccgaagc gtttgatagt tgatatcctt tgcctaattg taagtggaat gcttgcgacc
     8941 cagctttctt gtacaaagtg gtgatgggga tccactagtg agctcatgca tgatcgaatt
     9001 agcttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga
     9061 acttcaagat cccctgattc cctttgtcaa cagcaatgga taattcacga acccagttga
     9121 cataagcctg ttcggttcgt aaactgtaat gcaagtagcg tatgcgctca cgcaactggt
     9181 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     9241 atgactgttt ttttgtacag tctagcctcg ggcatccaag ctagctaagc gcgttacgcc
     9301 gtgggtcgat gtttgatgtt atggaacagc aacgatgtta cgcagcaggg tagtcgccct
     9361 aaaacaaagt taggcagccg ttgtgctggt gctttctagt agttgttgtg gggtaggcag
     9421 tcagagctcg atttgcttgt cgccataata gattcacaag aaggattcga catgggtcaa
     9481 agtagcgatg aagccaacgc tcccgttgca gggcagtttg cgcttcccct gagtgccacc
     9541 tttggcttag gggatcgcgt acgcaagaaa tctggtgccg cttggcaggg tcaagtcgtc
     9601 ggttggtatt gcacaaaact cactcctgaa ggctatgcgg tcgagtccga atcccaccca
     9661 ggctcagtgc aaatttatcc tgtggctgca cttgaacgtg tggcctaaga attatctagg
     9721 attattccat tgagtaagtt tttaagcaca tcagcttcaa aagcgctctg aagttcctat
     9781 actttctaga gaataggaac ttcggaatag gtacttcaag atccccaatt cgatcgtccg
     9841 ggccgcaagc tcctagcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     9901 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctcaagc
     9961 tagagagtca ttaccccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc
    10021 ccgccctgcc actcatcgca gtctagcttg gattctcacc aataaaaaac gcccggcggc
    10081 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
    10141 ggagtccaag ctcagctaat taagctagct tatcgatacc gtcgacctcg aaccccacgc
    10201 ccctctttaa tacgacgggc aatttgcact tcagaaaatg aagagtttgc tttagccata
    10261 acaaaagtcc agtatgcttt ttcacagcat aactggactg atttcagttt acaactattc
    10321 tgtctagttt aagactttat tgtcatagtt tagatctatt ttgttcagtt taagacttta
    10381 ttgtccgccc acacccgctt acgcagggca tccatttatt actcaaccgt aaccgatttt
    10441 gccaggttac gcggctggtc ctctagctgg cgtaatagcg aagaggcccg caccgatcgc
    10501 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
    10561 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
    10621 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
    10681 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
    10741 cagaggtttt caccgtcatc accgaaacgc gcga
//
