pTK1956 vector (V002623)

LOCUS       V002623                10256 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002623
VERSION     V002623
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10256)
  AUTHORS   Kafri T.
  TITLE     Superior lentiviral vectors designed for BSL-0 environment abolish
            vector mobilization
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10256)
  AUTHORS   Kafri T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2018) Gene Therapy Center, University of North
            Carolina at Chapel Hill, 104 Manning Dr., Chapel Hill, NC 27599, US
REFERENCE   3  (bases 1 to 10256)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10256)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-MAY-2018) Gene Therapy Center, University of North Carolina at
            Chapel Hill, 104 Manning Dr., Chapel Hill, NC 27599, US"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..817
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             832..1012
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1674..1907
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2092..2136
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2285..2326
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2395..2512
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        complement(2530..2547)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    complement(2565..2686)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(2908..3496)
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(3581..5230)
                     /label="luciferase"
                     /note="firefly luciferase"
     regulatory      5267..5672
                     /label="hAAT"
                     /note="hAAT"
                     /regulatory_class="promoter"
     repeat_region   5947..6238
                     /label="3' LTR"
                     /note="3' LTR"
     LTR             6057..6237
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6269..6493
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6539..6967
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6981..7311
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        7359..7406
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             7425..7796
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    7929..8062
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8099..8115)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    8123..8139
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8147..8177)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8192..8213)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8501..9089)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9263..10120)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10121..10225)
                     /label="AmpR promoter"