LOCUS       V002623                10256 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002623
VERSION     V002623
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10256)
  AUTHORS   Kafri T.
  TITLE     Superior lentiviral vectors designed for BSL-0 environment abolish
            vector mobilization
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10256)
  AUTHORS   Kafri T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2018) Gene Therapy Center, University of North
            Carolina at Chapel Hill, 104 Manning Dr., Chapel Hill, NC 27599, US
REFERENCE   3  (bases 1 to 10256)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10256)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-MAY-2018) Gene Therapy Center, University of North Carolina at
            Chapel Hill, 104 Manning Dr., Chapel Hill, NC 27599, US"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..817
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             832..1012
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1674..1907
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2092..2136
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2285..2326
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2395..2512
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        complement(2530..2547)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    complement(2565..2686)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(2908..3496)
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(3581..5230)
                     /label="luciferase"
                     /note="firefly luciferase"
     regulatory      5267..5672
                     /label="hAAT"
                     /note="hAAT"
                     /regulatory_class="promoter"
     repeat_region   5947..6238
                     /label="3' LTR"
                     /note="3' LTR"
     LTR             6057..6237
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6269..6493
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6539..6967
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6981..7311
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        7359..7406
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             7425..7796
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    7929..8062
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8099..8115)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    8123..8139
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8147..8177)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8192..8213)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8501..9089)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9263..10120)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10121..10225)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1861 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcccaatt gcaattttaa
     2401 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2461 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgtagggc
     2521 ggccgcttcc ctttagtgag ggttaatgct tcgagcagac atgataagat acattgatga
     2581 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2641 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2701 cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa
     2761 cctctacaaa tgtggtaaaa tccgataagg atcgatccgg gctggcgtaa tagcgaagag
     2821 gcccgcaccg atcgcccttc ccaacagttg ctactgcgca gcccgggctt cacggtttcg
     2881 tcgaccgtgc acccaactgc gtacgatgcg gggaggcggc ccaaagggag atccgactcg
     2941 tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag
     3001 gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc
     3061 aggtggcaac acaggcgagc agccatggaa aggacgtcag cttccccgac aacaccacgg
     3121 aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga
     3181 gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg
     3241 tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc acgccacgtt
     3301 gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta tacaaggagg
     3361 agaaaatgaa agccatacgg gaagcaatag catgatacaa aggcattaaa gcagcgtatc
     3421 cacatagcgt aaaaggagca acatagttaa gaataccagt caatctttca caaattttgt
     3481 aatccagagg ttgattatcg tacgggcgcg tcgagttgat cccccctaga gtattacaat
     3541 agctaagaat ttcgtcatcg ctgaatacag ttacatttta caatttggac tttccgccct
     3601 tcttggcctt tatgaggatc tctctgattt ttcttgcgtc gagttttccg gtaagacctt
     3661 tcggtacttc gtccacaaac acaactcctc cgcgcaactt tttggcggtt gttacttgac
     3721 tggcgacgta atccacgatc tctttttccg tcatcgtctt tccgtgctcc aaaacaacaa
     3781 cggcggcggg aagttcaccg gcgtcatcgt cgggaagacc tgccacgccc gcgtcgaaga
     3841 tgttggggtg ttgtaacaat atcgactcca attcagcggg ggccacctgg tatcctttgt
     3901 atttaattaa agacttcaag cggtcaacta tgaagaagtg ttcgtcttcg tcccagtaag
     3961 ctatgtctcc agaatgtagc catccatcct tgtcaatcaa ggcgttggtc gcttccggat
     4021 tgtttacata accggacata atcataggtc ctctgacaca taattcgcct ctctgattaa
     4081 cgcccagcgt tttcccggta tccagatcca caaccttcgc ttcaaaaaat ggaacaactt
     4141 taccgaccgc gcccggttta tcatccccct cgggtgtaat cagaatagct gatgtagtct
     4201 cagtgagccc atatccttgt cgtatccctg gaagatggaa gcgttttgca accgcttccc
     4261 cgacttcttt cgaaagaggt gcgcccccag aagcaatttc gtgtaaatta gataaatcgt
     4321 atttgtcaat cagagtgctt ttggcgaaga atgaaaatag ggttggtact agcaacgcac
     4381 tttgaatttt gtaatcctga agggatcgta aaaacagctc ttcttcaaat ctatacatta
     4441 agacgactcg aaatccacat atcaaatatc cgagtgtagt aaacattcca aaaccgtgat
     4501 ggaatggaac aacacttaaa atcgcagtat ccggaatgat ttgattgcca aaaataggat
     4561 ctctggcatg cgagaatctg acgcaggcag ttctatgcgg aagggccaca cccttaggta
     4621 acccagtaga tccagaggag ttcattatca gtgcaattgt tttgtcacga tcaaaggact
     4681 ctggtacaaa atcgtattca ttaaaaccgg gaggtagatg agatgtgacg aacgtgtaca
     4741 tcgactgaaa tccctggtaa tccgttttag aatccatgat aataattttc tggattattg
     4801 gtaatttttt ttgcacgttc aaaatttttt gcaacccctt tttggaaaca aacactacgg
     4861 taggctgcga aatgttcata ctgttgagca attcacgttc attataaatg tcgttcgcgg
     4921 gcgcaactgc aactccgata aataacgcgc ccaacaccgg cataaagaat tgaagagagt
     4981 tttcactgca tacgacgatt ctgtgatttg tattcagccc atatcgtttc atagcttctg
     5041 ccaaccgaac ggacatttcg aagtattccg cgtacgtgat gttcacctcg atatgtgcat
     5101 ctgtaaaagc aattgttcca ggaaccaggg cgtatctctt catagcctta tgcagttgct
     5161 ctccagcggt tccatcctct agcggataga atggcgccgg gcctttcttt atgtttttgg
     5221 cgtcttccat ggtacgggct gcaggaatta actctagtgc ggccgcagat cagggggatc
     5281 attcactgtc ccaggtcagt ggtggtgcct gaagctgagg agacagggcc ctgtcctcgt
     5341 ccgtatttaa gcagtggatc cagaggggca acgggggagg ctgctggtga atattaacca
     5401 aggtcacccc agttatcgga ggagcaaaca ggggctaagt ccactggctg ggatctgagt
     5461 cgcccgccta cgctgcccgg acgctttgcc tgggcagtgt acagcttcca ctgcacttac
     5521 cgaaaggagt cattgtacct ggctcagaaa ccacagcgtc ctgtgtccaa ggtggagggg
     5581 gtggcgtgag tcagacagtc tctgggagag taccacttag ctggccctct gctctcactg
     5641 cagaatcctt agtggctgtt ccactggtag caagatctag aatcgatgaa ttctgcagcc
     5701 accgcggtgg agctcgagga cccttgtcgg atcaagctta tcgatctaga ccgtcgagac
     5761 ctggaaaaac atggagcaat cacaagtagc aacacagcag ctaccaatgc tgcttgtgcc
     5821 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca tctagaggta
     5881 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     5941 gggggactgg aagggctgct gaattcactc ccaacgaaga caagatatcc actgaccgca
     6001 cgcctgcagg ctagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     6061 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6121 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6181 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     6241 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     6301 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     6361 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     6421 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     6481 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     6541 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6601 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6661 ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt
     6721 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6781 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6841 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6901 gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6961 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     7021 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     7081 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     7141 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     7201 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     7261 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     7321 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
     7381 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
     7441 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
     7501 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
     7561 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
     7621 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
     7681 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
     7741 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
     7801 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     7861 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     7921 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7981 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8041 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     8101 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8161 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8221 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8281 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8341 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8401 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8461 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8521 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8581 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8641 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     8701 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8761 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8821 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8881 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8941 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9001 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9061 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9121 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9181 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9241 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9301 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9361 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9421 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9481 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9541 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9601 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9661 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9721 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9781 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9841 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9901 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9961 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10021 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10081 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10141 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    10201 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
