pTIE1 vector (V002647)

LOCUS       40924_43353        3942 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Insect cell expression vector pTIE1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Crawford F, Huseby E, White J, Marrack P, Kappler JW.
  TITLE     Mimotopes for alloreactive and conventional T cells in a peptide-MHC
            display library
  JOURNAL   PLoS Biol. 2 (4), E90 (2004)
  PUBMED    15094798
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Kappler J.
  TITLE     pTIE1 - hr5/IE1 Based Insect Cell Expression Vector
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3942)
  AUTHORS   Kappler J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2004) Integrated Department of Immunology, 
            HHMI-National Jewish Research and Medical Center, 1400 Jackson St., 
            Denver, CO 80206, USA
REFERENCE   4  (bases 1 to 3942)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3942)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2004"; volume: "2"; issue: "4"; pages: "E90"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (09-JAN-2004) Integrated Department of Immunology, HHMI-National 
            Jewish Research and Medical Center, 1400 Jackson St., Denver, CO 
            80206, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        236..254
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        313..795
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        833..1424
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus
                     Autographa californica"
     misc_feature    1430..1530
                     /label=multiple cloning site
                     /note="multiple cloning site"
     3'UTR           1531..1779
                     /label=Baculovirus IE1 3' UTR
                     /note="Baculovirus IE1 3' UTR"
     promoter        2043..2147
                     /label=AmpR promoter
     CDS             2148..3005
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3179..3767
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"