LOCUS       40924_43353        3942 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Insect cell expression vector pTIE1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Crawford F, Huseby E, White J, Marrack P, Kappler JW.
  TITLE     Mimotopes for alloreactive and conventional T cells in a peptide-MHC
            display library
  JOURNAL   PLoS Biol. 2 (4), E90 (2004)
  PUBMED    15094798
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Kappler J.
  TITLE     pTIE1 - hr5/IE1 Based Insect Cell Expression Vector
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3942)
  AUTHORS   Kappler J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2004) Integrated Department of Immunology, 
            HHMI-National Jewish Research and Medical Center, 1400 Jackson St., 
            Denver, CO 80206, USA
REFERENCE   4  (bases 1 to 3942)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3942)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2004"; volume: "2"; issue: "4"; pages: "E90"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (09-JAN-2004) Integrated Department of Immunology, HHMI-National 
            Jewish Research and Medical Center, 1400 Jackson St., Denver, CO 
            80206, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        236..254
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        313..795
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        833..1424
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus
                     Autographa californica"
     misc_feature    1430..1530
                     /label=multiple cloning site
                     /note="multiple cloning site"
     3'UTR           1531..1779
                     /label=Baculovirus IE1 3' UTR
                     /note="Baculovirus IE1 3' UTR"
     promoter        2043..2147
                     /label=AmpR promoter
     CDS             2148..3005
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3179..3767
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gaatttaata
      241 cgactcacta tagggaattg ggatgaattc atttgttttt aaaattgaac tggctttacg
      301 agtagaattc tacgcgtaaa acacaatcaa gtatgagtca taagctgatg tcatgttttg
      361 cacacggctc ataaccgaac tggctttacg agtagaattc tacttgtaac gcacgatcag
      421 tggatgatgt catttgtttt tcaaatcgag atgatgtcat gttttgcaca cggctcataa
      481 actcgcttta cgagtagaat tctacgtgta acgcacgatc gattgatgag tcatttgttt
      541 tgcaatatga tatcatacaa tatgactcat ttgtttttca aaaccgaact tgatttacgg
      601 gtagaattct acttgtaaag cacaatcaaa aagatgatgt catttgtttt tcaaaactga
      661 actcgcttta cgagtagaat tctacgtgta aaacacaatc aagaaatgat gtcatttgtt
      721 ataaaaataa aagctgatgt catgttttgc acatggctca taactaaact cgctttacgg
      781 gtagaattct acgcgtaaaa catgattgat aattaaataa ttgaattcaa ttcgatgtct
      841 ttgtgatgcg cgcgacattt ttgtaggtta ttgataaaat gaacggatac gttgcccgac
      901 attatcatta aatccttggc gtagaatttg tcgggtccat tgtccgtgtg cgctagcatg
      961 cccgtaacgg acctcgtact tttggcttca aaggttttgc gcacagacaa aatgtgccac
     1021 acttgcagct ctgcatgtgt gcgcgttacc acaaatccca acggcgcagt gtacttgttg
     1081 tatgcaaata aatctcgata aaggcgcggc gcgcgaatgc agctgatcac gtacgctcct
     1141 cgtgttccgt tcaaggacgg tgttatcgac ctcagattaa tgtttatcgg ccgactgttt
     1201 tcgtatccgc tcaccaaacg cgtttttgca ttaacattgt atgtcggcgg atgttctata
     1261 tctaatttga ataaataaac gataaccgcg ttggttttag agggcataat aaaagaaata
     1321 ttgttatcgt gttcgccatt agggcagtat aaattgacgt tcatgttgga tattgtttca
     1381 gttgcaagtt gacactggcg gcgacaagat cgtgaacaac caagtgacta agctggagac
     1441 gcaattgggg tcgacggctc gagggtgtac agttaactag tccagcatgc tgggatccgg
     1501 agcggccgcg gtctagagaa gacccgatcg tatacatata ttttgaattt aattaattat
     1561 acatatattt tatattattt ttgtctttta ttatcgaggg gccgctgttg gtgtggggtt
     1621 ttgcatagaa ataacaatgg gagttggcga cgttgctgcg ccaacaccac ctcctcctcc
     1681 tcctttcatc atgtatctgt agataaaata aaatattaaa cctaaaaaca agaccgcgcc
     1741 tatcaacaaa atgataggca ttaacttgcc gctgacgctc actacgtgaa ccatcaccct
     1801 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     1861 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     1921 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     1981 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     2041 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     2101 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     2161 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     2221 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     2281 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     2341 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     2401 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     2461 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     2521 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     2581 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     2641 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     2701 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     2761 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     2821 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     2881 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     2941 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     3001 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     3061 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     3121 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3181 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3241 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3301 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     3361 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3421 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3481 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3541 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     3601 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3661 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3721 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3781 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3841 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3901 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga ag
//
