pTH1522 vector (V002724)

LOCUS       40924_43203       10705 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTH1522, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10705)
  AUTHORS   Cowie A, Cheng J, Sibley CD, Fong Y, Zaheer R, Patten CL, Morton RM,
            Golding GB, Finan TM.
  TITLE     An integrated approach to functional genomics: construction of a 
            novel reporter gene fusion library for Sinorhizobium meliloti
  JOURNAL   Appl. Environ. Microbiol. 72 (11), 7156-7167 (2006)
  PUBMED    16963549
REFERENCE   2  (bases 1 to 10705)
  AUTHORS   Cheng J, Finan TM.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2005) Department of Biology, McMaster University, 
            1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada
REFERENCE   3  (bases 1 to 10705)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10705)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2006"; volume: "72"; issue: "11"; 
            pages: "7156-7167"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-DEC-2005) Department of Biology, McMaster University, 1280 Main 
            Street West, Hamilton, Ontario L8S 4K1, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(15..48)
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     regulatory      66..93
                     /label=trpA
                     /note="trpA"
                     /regulatory_class="terminator"
     regulatory      complement(121..170)
                     /label=phiC31 attP attachment site
                     /note="phiC31 attP attachment site"
                     /regulatory_class="other"
     CDS             complement(180..3224)
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             complement(3291..4007)
                     /label=Cycle 3 GFP
                     /note="Cycle 3 GFP (Crameri et al., 1996)"
     misc_feature    complement(4019..4029)
                     /label=stop codons
                     /note="stop codons"
     misc_feature    4042..4052
                     /label=stop codons
                     /note="stop codons"
     CDS             4100..5908
                     /codon_start=1
                     /gene="gusA"
                     /product="beta-glucuronidase"
                     /label=gusA
                     /protein_id="ABC47320.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQVHGNISPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            4100..5908
                     /gene="gusA"
                     /label=gusA
     CDS             5945..6622
                     /label=dTomato
                     /note="dimeric variant of DsRed fluorescent protein (Shaner
                     et al., 2004)"
     regulatory      7366..7505
                     /label=omega terminator
                     /note="omega terminator"
                     /regulatory_class="terminator"
     CDS             7827..8591
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     regulatory      complement(8983..9122)
                     /label=omega terminator
                     /note="omega terminator"
                     /regulatory_class="terminator"
     rep_origin      9306..9894
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(10080..10220)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(10325..10513)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"