LOCUS       40924_43203       10705 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTH1522, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10705)
  AUTHORS   Cowie A, Cheng J, Sibley CD, Fong Y, Zaheer R, Patten CL, Morton RM,
            Golding GB, Finan TM.
  TITLE     An integrated approach to functional genomics: construction of a 
            novel reporter gene fusion library for Sinorhizobium meliloti
  JOURNAL   Appl. Environ. Microbiol. 72 (11), 7156-7167 (2006)
  PUBMED    16963549
REFERENCE   2  (bases 1 to 10705)
  AUTHORS   Cheng J, Finan TM.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2005) Department of Biology, McMaster University, 
            1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada
REFERENCE   3  (bases 1 to 10705)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10705)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2006"; volume: "72"; issue: "11"; 
            pages: "7156-7167"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-DEC-2005) Department of Biology, McMaster University, 1280 Main 
            Street West, Hamilton, Ontario L8S 4K1, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(15..48)
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     regulatory      66..93
                     /label=trpA
                     /note="trpA"
                     /regulatory_class="terminator"
     regulatory      complement(121..170)
                     /label=phiC31 attP attachment site
                     /note="phiC31 attP attachment site"
                     /regulatory_class="other"
     CDS             complement(180..3224)
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             complement(3291..4007)
                     /label=Cycle 3 GFP
                     /note="Cycle 3 GFP (Crameri et al., 1996)"
     misc_feature    complement(4019..4029)
                     /label=stop codons
                     /note="stop codons"
     misc_feature    4042..4052
                     /label=stop codons
                     /note="stop codons"
     CDS             4100..5908
                     /codon_start=1
                     /gene="gusA"
                     /product="beta-glucuronidase"
                     /label=gusA
                     /protein_id="ABC47320.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQVHGNISPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            4100..5908
                     /gene="gusA"
                     /label=gusA
     CDS             5945..6622
                     /label=dTomato
                     /note="dimeric variant of DsRed fluorescent protein (Shaner
                     et al., 2004)"
     regulatory      7366..7505
                     /label=omega terminator
                     /note="omega terminator"
                     /regulatory_class="terminator"
     CDS             7827..8591
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     regulatory      complement(8983..9122)
                     /label=omega terminator
                     /note="omega terminator"
                     /regulatory_class="terminator"
     rep_origin      9306..9894
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(10080..10220)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(10325..10513)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
ORIGIN
        1 ggtaccgcat gcttgaagtt cctatacttt ctagagaata ggaacttctg caggtcgaga
       61 tccccagccc gcctaatgag cgggcttttt tttggtcgac accggtcacg tgcctaggac
      121 ctacgccccc aactgagaga actcaaaggt taccccagtt ggggcactac tagttattat
      181 ttttgacacc agaccaactg gtaatggtag cgaccggcgc tcagctgtaa ttccgccgat
      241 actgacgggc tccaggagtc gtcgccacca atccccatat ggaaaccgtc gatattcagc
      301 catgtgcctt cttccgcgtg cagcagatgg cgatggctgg tttccatcag ttgctgttga
      361 ctgtagcggc tgatgttgaa ctggaagtcg ccgcgccact ggtgtgggcc ataattcaat
      421 tcgcgcgtcc cgcagcgcag accgttttcg ctcgggaaga cgtacggggt atacatgtct
      481 gacaatggca gatcccagcg gtcaaaacag gcggcagtaa ggcggtcggg atagttttct
      541 tgcggcccta atccgagcca gtttacccgc tctgctacct gcgccagctg gcagttcagg
      601 ccaatccgcg ccggatgcgg tgtatcgctc gccacttcaa catcaacggt aatcgccatt
      661 tgaccactac catcaatccg gtaggttttc cggctgataa ataaggtttt cccctgatgc
      721 tgccacgcgt gagcggtcgt aatcagcacc gcatcagcaa gtgtatctgc cgtgcactgc
      781 aacaacgctg cttcggcctg gtaatggccc gccgccttcc agcgttcgac ccaggcgtta
      841 gggtcaatgc gggtcgcttc acttacgcca atgtcgttat ccagcggtgc acgggtgaac
      901 tgatcgcgca gcggcgtcag cagttgtttt ttatcgccaa tccacatctg tgaaagaaag
      961 cctgactggc ggttaaattg ccaacgctta ttacccagct cgatgcaaaa atccatttcg
     1021 ctggtggtca gatgcgggat ggcgtgggac gcggcgggga gcgtcacact gaggttttcc
     1081 gccagacgcc actgctgcca ggcgctgatg tgcccggctt ctgaccatgc ggtcgcgttc
     1141 ggttgcacta cgcgtactgt gagccagagt tgcccggcgc tctccggctg cggtagttca
     1201 ggcagttcaa tcaactgttt accttgtgga gcgacatcca gaggcacttc accgcttgcc
     1261 agcggcttac catccagcgc caccatccag tgcaggagct cgttatcgct atgacggaac
     1321 aggtattcgc tggtcacttc gatggtttgc ccggataaac ggaactggaa aaactgctgc
     1381 tggtgttttg cttccgtcag cgctggatgc ggcgtgcggt cggcaaagac cagaccgttc
     1441 atacagaact ggcgatcgtt cggcgtatcg ccaaaatcac cgccgtaagc cgaccacggg
     1501 ttgccgtttt catcatattt aatcagcgac tgatccaccc agtcccagac gaagccgccc
     1561 tgtaaacggg gatactgacg aaacgcctgc cagtatttag cgaaaccgcc aagactgtta
     1621 cccatcgcgt gggcgtattc gcaaaggatc agcgggcgcg tctctccagg tagcgaaagc
     1681 cattttttga tggaccattt cggcacagcc gggaagggct ggtcttcatc cacgcgcgcg
     1741 tacatcgggc aaataatatc ggtggccgtg gtgtcggctc cgccgccttc atactgcacc
     1801 gggcgggaag gatcgacaga tttgatccag cgatacagcg cgtcgtgatt agcgccgtgg
     1861 cctgattcat tccccagcga ccagatgatc acactcgggt gattacgatc gcgctgcacc
     1921 attcgcgtta cgcgttcgct catcgccggt agccagcgcg gatcatcggt cagacgattc
     1981 attggcacca tgccgtgggt ttcaatattg gcttcatcca ccacatacag gccgtagcgg
     2041 tcgcacagcg tgtaccacag cggatggttc ggataatgcg aacagcgcac ggcgttaaag
     2101 ttgttctgct tcatcagcag gatatcctgc accatcgtct gctcatccat gacctgacca
     2161 tgcagaggat gatgctcgtg acggttaacg cctcgaatca gcaacggctt gccgttcagc
     2221 agcagcagac cattttcaat ccgcacctcg cggaaaccga catcgcaggc ttctgcttca
     2281 atcagcgtgc cgtcggcggt gtgcagttca accaccgcac gatagagatt cgggatttcg
     2341 gcgctccaca gtttcgggtt ttcgacgttc agacgtagtg tgacgcgatc ggcataacca
     2401 ccacgctcat cgataatttc accgccgaaa ggcgcggtgc cgctggcgac ctgcgtttca
     2461 ccctgccata aagaaactgt tacccgtagg tagtcacgca actcgccgca catctgaact
     2521 tcagcctcca gtacagcgcg gctgaaatca tcattaaagc gagtggcaac atggaaatcg
     2581 ctgatttgtg tagtcggttt atgcagcaac gagacgtcac ggaaaatgcc gctcatccgc
     2641 cacatatcct gatcttccag ataactgccg tcactccagc gcagcaccat caccgcgagg
     2701 cggttttctc cggcgcgtaa aaatgcgctc aggtcaaatt cagacggcaa acgactgtcc
     2761 tggccgtaac cgacccagcg cccgttgcac cacagatgaa acgccgagtt aacgccatca
     2821 aaaataattc gcgtctggcc ttcctgtagc cagctttcat caacattaaa tgtgagcgag
     2881 taacaacccg tcggattctc cgtgggaaca aacggcggat tgaccgtaat gggataggtc
     2941 acgttggtgt agatgggcgc atcgtaaccg tgcatctgcc agtttgaggg gacgacgaca
     3001 gtatcggcct caggaagatc gcactccagc cagctttccg gcaccgcttc tggtgccgga
     3061 aaccaggcaa agcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     3121 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     3181 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacgggatc gatcacagaa
     3241 gaagtagtgt tacccagagt cagcattatc tagctcctca gatctcatta tttgtagagc
     3301 tcatccatgc catgtgtaat cccagcagca gttacaaact caagaaggac catgtggtca
     3361 cgcttttcgt tgggatcttt cgaaagggca gattgtgtcg acaggtaatg gttgtctggt
     3421 aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc tagttgaacg
     3481 gatccatctt caatgttgtg gcgaattttg aagttagctt tgattccatt cttttgtttg
     3541 tctgccgtga tgtatacatt gtgtgagtta tagttgtact ctagtttgtg tccgagaatg
     3601 tttccatctt ctttaaaatc aatacctttt aactcgatac gattaacaag ggtatcacct
     3661 tcaaacttga cttcagcacg cgtcttgtag ttcccgtcat ctttgaaaga tatagtgcgt
     3721 tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgccgttt catatgatcc
     3781 ggataacggg aaaagcattg aacaccatag gtcaaagtag tgacaagtgt tggccatgga
     3841 acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gctttccgta tgtagcatca
     3901 ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc taattcaaca
     3961 agaattggga caactccagt gaaaagttct tctcctttgc tagccattat taatctcctc
     4021 aatcaatcac tcgagatccg gtgattgatt gagcaagcta attccggacc agtattgtta
     4081 tcttaatgag gagtccctta tgttacgtcc tgtagaaacc ccaacccgtg aaatcaaaaa
     4141 actcgacggc ctgtgggcat tcagtctgga tcgcgaaaac tgtggaattg atcagcgttg
     4201 gtgggaaagc gcgttacaag aaagccgggc aattgctgtg ccaggcagtt ttaacgatca
     4261 gttcgccgat gcagatattc gtaattatgc gggcaacgtc tggtatcagc gcgaagtctt
     4321 tataccgaaa ggttgggcag gccagcgtat cgtgctgcgt ttcgatgcgg tcactcatta
     4381 cggcaaagtg tgggtcaata atcaggaagt gatggagcat cagggcggct atacgccatt
     4441 tgaagccgat gtcacgccgt atgttattgc cgggaaaagt gtacgtatca ccgtttgtgt
     4501 gaacaacgaa ctgaactggc agactatccc gccgggaatg gtgattaccg acgaaaacgg
     4561 caagaaaaag cagtcttact tccatgattt ctttaactat gccgggatcc atcgcagcgt
     4621 aatgctctac accacgccga acacctgggt ggacgatatc accgtggtga cgcatgtcgc
     4681 gcaagactgt aaccacgcgt ctgttgactg gcaggtggtg gccaatggtg atgtcagcgt
     4741 tgaactgcgt gatgcggatc aacaggtggt tgcaactgga caaggcacta gcgggacttt
     4801 gcaagtggtg aatccgcacc tctggcaacc gggtgaaggt tatctctatg aactgtgcgt
     4861 cacagccaaa agccagacag agtgtgatat ctacccgctt cgcgtcggca tccggtcagt
     4921 ggcagtgaag ggcgaacagt tcctgattaa ccacaaaccg ttctacttta ctggctttgg
     4981 tcgtcatgaa gatgcggact tacgtggcaa aggattcgat aacgtgctga tggtgcacga
     5041 ccacgcatta atggactgga ttggggccaa ctcctaccgt acctcgcatt acccttacgc
     5101 tgaagagatg ctcgactggg cagatgaaca tggcatcgtg gtgattgatg aaactgctgc
     5161 tgtcggcttt aacctctctt taggcattgg tttcgaagcg ggcaacaagc cgaaagaact
     5221 gtacagcgaa gaggcagtca acggggaaac tcagcaagcg cacttacagg cgattaaaga
     5281 gctgatagcg cgtgacaaaa accacccaag cgtggtgatg tggagtattg ccaacgaacc
     5341 ggatacccgt ccgcaagtgc acgggaatat ttcgccactg gcggaagcaa cgcgtaaact
     5401 cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac
     5461 catcagcgat ctctttgatg tgctgtgcct gaaccgttat tacggatggt atgtccaaag
     5521 cggcgatttg gaaacggcag agaaggtact ggaaaaagaa cttctggcct ggcaggagaa
     5581 actgcatcag ccgattatca tcaccgaata cggcgtggat acgttagccg ggctgcactc
     5641 aatgtacacc gacatgtgga gtgaagagta tcagtgtgca tggctggata tgtatcaccg
     5701 cgtctttgat cgcgtcagcg ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc
     5761 gacctcgcaa ggcatattgc gcgttggcgg taacaagaaa gggatcttca ctcgcgaccg
     5821 caaaccgaag tcggcggctt ttctgctgca aaaacgctgg actggcatga acttcggtga
     5881 aaaaccgcag cagggaggca aacaatgaat caacaactct cctgcagaat tcgaggaggt
     5941 tccgatggtg gcctcctccg aggacgtcat caaagagttc atgcgcttca aggtgcgcat
     6001 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     6061 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
     6121 ggacatcctg tccccccagt tccagtacgg ctccaaggcg tacgtgaagc accccgccga
     6181 catccccgac tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     6241 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct
     6301 gatctacaag gtgaagttcc gcggcaccaa cttccccccc gacggccccg taatgcagaa
     6361 gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa
     6421 gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa
     6481 gaccatctac atggccaaga agcccgtgca gctgcccggc tactactacg tggacaccaa
     6541 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga
     6601 gggccgccac cacctgttcc tggggcatgg caccggcagc accggcagcg gcagctccgg
     6661 caccgcctcc tccgaggacg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg
     6721 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     6781 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
     6841 cctgtccccc cagttccagt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc
     6901 cgactacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     6961 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta
     7021 caaggtgaag ttccgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac
     7081 catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga
     7141 gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat
     7201 ctacatggcc aagaagcccg tgcagctgcc cggctactac tacgtggaca ccaagctgga
     7261 catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg
     7321 ccaccacctg ttcctgtgag aagtcgaagc ttgacccggg gatccggtga ttgattgagc
     7381 aagctttatg cttgtaaacc gttttgtgaa aaaattttta aaataaaaaa ggggacctct
     7441 agggtcccca attaattagt aatataatct attaaaggtc attcaaaagg tcatccaccg
     7501 gatcaattcc cctgctcgcg caggctgggt gccaagctct cgggtaacat caaggcccga
     7561 tccttggagc ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg
     7621 acccgcagtt gcaaaccctc actgatccgg ctcacggtaa ctgatgccgt atttgcagta
     7681 ccagcgtacg gcccacagaa tgatgtcacg ctgaaaatgc cggcctttga atgggttcat
     7741 gtgcagctcc atcagcaaaa ggggatgata agtttatcac caccgactat ttgcaacagt
     7801 gccgttgatc gtgctatgat cgactgatgt catcagcggt ggagtgcaat gtcgtgcaat
     7861 acgaatggcg aaaagccgag ctcatcggtc agcttctcaa ccttggggtt acccccggcg
     7921 gtgtgctgct ggtccacagc tccttccgta gcgtccggcc cctcgaagat gggccacttg
     7981 gactgatcga ggccctgcgt gctgcgctgg gtccgggagg gacgctcgtc atgccctcgt
     8041 ggtcaggtct ggacgacgag ccgttcgatc ctgccacgtc gcccgttaca ccggaccttg
     8101 gagttgtctc tgacacattc tggcgcctgc caaatgtaaa gcgcagcgcc catccatttg
     8161 cctttgcggc agcggggcca caggcagagc agatcatctc tgatccattg cccctgccac
     8221 ctcactcgcc tgcaagcccg gtcgcccgtg tccatgaact cgatgggcag gtacttctcc
     8281 tcggcgtggg acacgatgcc aacacgacgc tgcatcttgc cgagttgatg gcaaaggttc
     8341 cctatggggt gccgagacac tgcaccattc ttcaggatgg caagttggta cgcgtcgatt
     8401 atctggagaa tgaccactgc tgtgagcgct ttgccttggc ggacaggtgg ctcaaggaga
     8461 agagccttca gaaggaaggt ccagtcggtc atgcctttgc tcggttgatc cgctcccgcg
     8521 acattgtggc gacagccctg ggtcaactgg gccgagatcc gttgatcttc ctgcatccgc
     8581 cagagggcgg gatgcgaaga atgcgatgcc gctcgccagt cgattggctg agctcatgag
     8641 cggagaacga gatgacgttg gaggggcaag gtcgcgctga ttgctggggc aacacgtgaa
     8701 aggcgagatc accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg
     8761 cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca
     8821 aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa
     8881 ttgggagata tatcatgaaa ggctggcttt ttcttgttat cgcaatagtt ggcgaagtaa
     8941 tcgcaacatc cgcattaaaa tctagcgagg gctttactaa gctgatccgg tggatgacct
     9001 tttgaatgac ctttaataga ttatattact aattaattgg ggaccctaga ggtccccttt
     9061 tttattttaa aaattttttc acaaaacggt ttacaagcat aaagcttgct caatcaatca
     9121 ccggatcccc gggaattcat gcatgggccc ttaattaaat atactttaga ttgatttaaa
     9181 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     9241 aatcccttaa cgtgagtttt cgttccactg agcgtcaacc ccgtagaaaa gatcaaagga
     9301 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9361 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9421 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     9481 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     9541 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     9601 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     9661 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     9721 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9781 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9841 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     9901 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     9961 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    10021 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    10081 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
    10141 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
    10201 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
    10261 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
    10321 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag
    10381 cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt
    10441 tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt
    10501 cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac
    10561 cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac
    10621 tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca
    10681 ctcagggtca atgccagcgc ttcgt
//
