pTGPI-GFP vector (V002733)

LOCUS       V002733                10163 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002733
VERSION     V002733
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10163)
  AUTHORS   Meissner M, Krejany E, Gilson PR, de Koning-Ward TF, Soldati D,
            Crabb BS.
  TITLE     Tetracycline analogue-regulated transgene expression in Plasmodium
            falciparum blood stages using Toxoplasma gondii transactivators
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 102 (8), 2980-2985 (2005)
   PUBMED   15710888
REFERENCE   2  (bases 1 to 10163)
  AUTHORS   Meissner M, Krejany E, Gilson P, de Koning Ward T, Soldati D, Crabb
            B.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2004) Infection and Immunity, Walter and Eliza
            Hall Institute of Medical Research, Royal Parade, Melbourne,
            Victoria 3050, Australia
REFERENCE   3  (bases 1 to 10163)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10163)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2005"; volume: "102"; issue: "8"; pages:
            "2980-2985"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-DEC-2004) Infection and Immunity, Walter and Eliza Hall
            Institute of Medical Research, Royal Parade, Melbourne, Victoria
            3050, Australia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..1078
                     /label="calmodulin promoter"
                     /note="calmodulin promoter"
                     /regulatory_class="promoter"
     CDS             1079..1639
                     /gene="DHFR"
                     /label="Dihydrofolate reductase"
                     /note="Dihydrofolate reductase from Homo sapiens.
                     Accession#: P00374"
     regulatory      1641..2215
                     /label="HRP23 terminator"
                     /note="HRP23 terminator"
                     /regulatory_class="terminator"
     promoter        complement(2219..2236)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2243..2259)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2733..2837
                     /label="AmpR promoter"
     CDS             2838..3695
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3869..4457
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4745..4766
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4781..4811
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4819..4835
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4843..4859
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4877..4895
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4927..4943)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    4954..5224
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     regulatory      5240..5563
                     /label="minimal calmodulin promoter"
                     /note="minimal calmodulin promoter"
                     /regulatory_class="promoter"
     CDS             5648..6361
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      complement(7151..8075)
                     /note="Plasmodium berghei dihydrofolate reductase
                     terminator"
                     /regulatory_class="terminator"
     CDS             complement(8173..8775)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     regulatory      complement(8776..9652)
                     /note="Plasmodium falciparum merozoite surface protein 2
                     promoter"
                     /regulatory_class="promoter"
     repeat_region   9653..10163
                     /label="REP20 subtelomeric repeat region"
                     /note="REP20 subtelomeric repeat region"